Detailed view for Tb927.7.3770

Basic information

TDR Targets ID: 14391
Trypanosoma brucei, Bifunctional NAD(P)H-hydrate repair enzyme
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.2412 | Length (AA): 575 | MW (Da): 61112 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01256   Carbohydrate kinase
PF03853   YjeF-related protein N-terminus

Gene Ontology

Mouse over links to read term descriptions.
GO:0052855   GO:ADP-dependent NAD(P)H-hydrate dehydratase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
34 566 3rss (A) 1 489 31.00 0 1 1.16895 0.16
46 559 3rss (A) 13 482 37.00 0 1 1.18226 0.3
68 159 2q41 (A) 118 201 38.00 0.098 0.58 0.444622 0.23
280 559 2r3b (A) 21 267 31.00 0 1 0.771311 -0.35

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127151)

Species Accession Gene Product
Arabidopsis thaliana AT5G19150   pfkB-like carbohydrate kinase family protein
Brugia malayi Bm1_07040   YjeF-related protein, C-terminus containing protein
Candida albicans CaO19.11002   similar to S. cerevisiae YKL151C
Candida albicans CaO19.3508   similar to S. cerevisiae YKL151C
Caenorhabditis elegans CELE_R107.2   Protein R107.2
Cryptosporidium hominis Chro.70406   ENSANGP00000015295
Cryptosporidium parvum cgd7_3640   YjeF family of predicted nucleotide binding proteins
Dictyostelium discoideum DDB_G0290799   uncharacterized protein family, carbohydrate kinase-related
Drosophila melanogaster Dmel_CG10424   CG10424 gene product from transcript CG10424-RA
Escherichia coli b4167   bifunctional NAD(P)H-hydrate repair enzyme
Echinococcus granulosus EgrG_000434000   carbohydrate kinase domain containing protein
Entamoeba histolytica EHI_194450   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000434000   carbohydrate kinase domain containing protein
Giardia lamblia GL50803_4255   Sugar kinase, putative
Homo sapiens ENSG00000213995   carbohydrate kinase domain containing
Leishmania braziliensis LbrM.14.1360   hypothetical protein, conserved
Leishmania donovani LdBPK_141270.1   Bifunctional NAD(P)H-hydrate repair enzyme
Leishmania infantum LinJ.14.1270   hypothetical protein, conserved
Leishmania major LmjF.14.1190   hypothetical protein, conserved
Leishmania mexicana LmxM.14.1190   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_04267   hypothetical protein
Mycobacterium leprae ML0373   Conserved hypothetical protein
Mus musculus ENSMUSG00000031505   carbohydrate kinase domain containing
Mycobacterium tuberculosis Rv3433c   Conserved protein
Mycobacterium ulcerans MUL_0877   transmembrane protein
Oryza sativa 4350275   Os11g0276300
Plasmodium berghei PBANKA_0905100   ATP-dependent (S)-NAD(P)H-hydrate dehydratase, putative
Plasmodium falciparum PF3D7_1143900   ATP-dependent (S)-NAD(P)H-hydrate dehydratase, putative
Plasmodium knowlesi PKNH_0941800   ATP-dependent (S)-NAD(P)H-hydrate dehydratase, putative
Plasmodium vivax PVX_092800   carbohydrate kinase, putative
Plasmodium yoelii PY02608   YjeF-related protein, C-terminus
Saccharomyces cerevisiae YKL151C   NADHX dehydratase
Schistosoma japonicum Sjp_0303960   similar to Uncharacterized protein FLJ10769 homolog precursor, putative
Schistosoma mansoni Smp_000470   hypothetical protein
Schmidtea mediterranea mk4.003659.00   ATP-dependent
Trypanosoma brucei gambiense Tbg972.7.4200   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.3770   Bifunctional NAD(P)H-hydrate repair enzyme
Trypanosoma congolense TcIL3000_7_3030   Bifunctional NAD(P)H-hydrate repair enzyme
Trypanosoma cruzi TcCLB.506795.44   Bifunctional NAD(P)H-hydrate repair enzyme
Trypanosoma cruzi TcCLB.509937.20   Bifunctional NAD(P)H-hydrate repair enzyme
Toxoplasma gondii TGME49_258160   carbohydrate kinase
Trichomonas vaginalis TVAG_468220   conserved hypothetical protein
Trichomonas vaginalis TVAG_051000   conserved hypothetical protein

Essentiality

Tb927.7.3770 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3494 Mycobacterium tuberculosis non-essential nmpdr
Tb927.7.3770 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.3770 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.3770 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.3770 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b4167 Escherichia coli non-essential goodall
PBANKA_0905100 Plasmodium berghei Dispensable plasmo
TGME49_258160 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.7.3770 (Trypanosoma brucei), Bifunctional NAD(P)H-hydrate repair enzyme
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