Detailed view for Tb927.4.3380

Basic information

TDR Targets ID: 14403
Trypanosoma brucei, myosin IB heavy chain, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.3232 | Length (AA): 1167 | MW (Da): 131763 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00063   Myosin head (motor domain)
PF00397   WW domain
PF06017   Unconventional myosin tail, actin- and lipid-binding

Gene Ontology

Mouse over links to read term descriptions.
GO:0016459   myosin complex  
GO:0008270   zinc ion binding  
GO:0008017   microtubule binding  
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0003777   microtubule motor activity  
GO:0003774   motor activity  
GO:0007018   microtubule-based movement  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126672)

Species Accession Gene Product
Babesia bovis BBOV_IV012030   myosin B
Babesia bovis BBOV_I003490   myosin A
Candida albicans CaO19.8357   myosin type 1
Candida albicans CaO19.738   myosin type 1
Caenorhabditis elegans CELE_F29D10.4   Protein HUM-1
Caenorhabditis elegans CELE_T02C12.1   Protein HUM-5
Cryptosporidium hominis Chro.70423   unconventional myosin-A
Cryptosporidium hominis Chro.70483   myosin B
Cryptosporidium parvum cgd7_3790   unconventional myosin
Cryptosporidium parvum cgd7_4350   myosinunconventional myosin fused to an RCC1 domain (unique)
Dictyostelium discoideum DDB_G0276617   class I myosin
Dictyostelium discoideum DDB_G0289117   class I myosin
Dictyostelium discoideum DDB_G0275447   class I myosin
Dictyostelium discoideum DDB_G0289177   class I myosin
Dictyostelium discoideum DDB_G0288679   class I myosin
Dictyostelium discoideum DDB_G0280039   class I myosin
Drosophila melanogaster Dmel_CG7438   Myosin 31DF
Drosophila melanogaster Dmel_CG5501   Myosin 95E
Drosophila melanogaster Dmel_CG9155   Myosin 61F
Entamoeba histolytica EHI_110810   unconventional myosin IB
Homo sapiens ENSG00000142347   myosin IF
Homo sapiens 4643   myosin IE
Homo sapiens ENSG00000166866   myosin IA
Homo sapiens ENSG00000174527   myosin IH
Homo sapiens ENSG00000136286   myosin IG
Homo sapiens ENSG00000176658   myosin ID
Homo sapiens ENSG00000197879   myosin IC
Homo sapiens ENSG00000128641   myosin IB
Leishmania braziliensis LbrM.20.0970   myosin IB heavy chain, putative
Leishmania donovani LdBPK_341070.1   myosin IB heavy chain, putative
Leishmania infantum LinJ.34.1070   myosin IB heavy chain, putative
Leishmania major LmjF.34.1000   myosin IB heavy chain, putative
Leishmania mexicana LmxM.33.1000   myosin IB heavy chain, putative
Loa Loa (eye worm) LOAG_06763   hypothetical protein
Mus musculus ENSMUSG00000066952   myosin 1H
Mus musculus ENSMUSG00000018417   myosin IB
Mus musculus ENSMUSG00000032220   myosin IE
Mus musculus ENSMUSG00000017774   myosin IC
Mus musculus ENSMUSG00000035441   myosin ID
Mus musculus ENSMUSG00000020437   myosin IG
Mus musculus ENSMUSG00000025401   myosin IA
Mus musculus ENSMUSG00000024300   myosin IF
Neospora caninum NCLIV_028750   hypothetical protein
Neospora caninum NCLIV_049900   hypothetical protein
Neospora caninum NCLIV_024740   Myosin, heavy polypeptide 1, skeletal muscle, adult, related
Neospora caninum NCLIV_018020   hypothetical protein
Neospora caninum NCLIV_016180   hypothetical protein
Plasmodium berghei PBANKA_1355700   myosin A
Plasmodium falciparum PF3D7_1342600   myosin A
Plasmodium knowlesi PKNH_1258800   myosin A, putative
Plasmodium vivax PVX_083030   myosin A, putative
Plasmodium yoelii PY01232   myosin A
Saccharomyces cerevisiae YKL129C   myosin 3
Saccharomyces cerevisiae YMR109W   myosin 5
Schmidtea mediterranea mk4.000662.04  
Schmidtea mediterranea mk4.013048.00   Myosin-IB
Schmidtea mediterranea mk4.000086.00  
Schmidtea mediterranea mk4.006357.03  
Schmidtea mediterranea mk4.005104.00   Hum-1
Trypanosoma brucei gambiense Tbg972.4.3390   myosin IB heavy chain, putative
Trypanosoma brucei Tb927.4.3380   myosin IB heavy chain, putative
Trypanosoma congolense TcIL3000_4_3080   myosin IB heavy chain, putative
Trypanosoma cruzi TcCLB.507739.110   myosin IB heavy chain, putative
Toxoplasma gondii TGME49_243250   myosin H
Toxoplasma gondii TGME49_239560   myosin E
Toxoplasma gondii TGME49_263180   myosin D
Toxoplasma gondii TGME49_255190   myosin C
Toxoplasma gondii TGME49_235470   myosin A
Theileria parva TP01_0500   myosin A, putative
Theileria parva TP01_1107   myosin B, putative

Essentiality

Tb927.4.3380 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.3380 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.3380 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.3380 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.3380 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1355700 Plasmodium berghei Essential plasmo
TGME49_255190 Toxoplasma gondii Probably essential sidik
TGME49_235470 Toxoplasma gondii Probably essential sidik
TGME49_239560 Toxoplasma gondii Probably essential sidik
TGME49_243250 Toxoplasma gondii Probably essential sidik
TGME49_263180 Toxoplasma gondii Probably essential sidik
TGME49_255190 Toxoplasma gondii Probably non-essential sidik
TGME49_235470 Toxoplasma gondii Probably non-essential sidik
TGME49_239560 Toxoplasma gondii Probably non-essential sidik
TGME49_243250 Toxoplasma gondii Probably non-essential sidik
TGME49_263180 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.4.3380 (Trypanosoma brucei), myosin IB heavy chain, putative
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