Detailed view for Tb927.4.1990

Basic information

TDR Targets ID: 14481
Trypanosoma brucei, ATP-dependent RNA and DNA helicase, mitochondrial, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.6875 | Length (AA): 626 | MW (Da): 70543 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00271   Helicase conserved C-terminal domain
PF12513   Mitochondrial degradasome RNA helicase subunit C terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0016817   hydrolase activity, acting on acid anhydrides  
GO:0005524   ATP binding  
GO:0004386   helicase activity  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128486)

Species Accession Gene Product
Arabidopsis thaliana AT5G39840   putative ATP-dependent RNA helicase
Arabidopsis thaliana AT4G14790   ATP-dependent RNA helicase
Babesia bovis BBOV_IV011410   ATP-dependent RNA helicase, putative
Brugia malayi Bm1_18110   Helicase conserved C-terminal domain containing protein
Candida albicans CaO19.11994   similar to putative RNA helicase
Candida albicans CaO19.4519   similar to putative RNA helicase
Caenorhabditis elegans CELE_C08F8.2   Protein C08F8.2, isoform C
Dictyostelium discoideum DDB_G0278937   hypothetical protein
Drosophila melanogaster Dmel_CG9791   CG9791 gene product from transcript CG9791-RC
Homo sapiens ENSG00000156502   suppressor of var1, 3-like 1 (S. cerevisiae)
Leishmania braziliensis LbrM.20.2180   RNA helicase, putative,mitochondrial, putative
Leishmania donovani LdBPK_342450.1   ATP-dependent RNA and DNA helicase, mitochondrial, putative
Leishmania infantum LinJ.34.2450   RNA helicase, putative,mitochondrial, putative
Leishmania major LmjF.34.2620   RNA helicase, putative,mitochondrial, putative
Leishmania mexicana LmxM.33.2620   RNA helicase, putative,mitochondrial, putative
Loa Loa (eye worm) LOAG_02037   hypothetical protein
Mus musculus ENSMUSG00000020079   suppressor of var1, 3-like 1 (S. cerevisiae)
Neospora caninum NCLIV_032500   hypothetical protein
Oryza sativa 4336047   Os04g0459800
Oryza sativa 4334089   Os03g0746500
Plasmodium berghei PBANKA_1122600   ATP-dependent RNA helicase SUV3, putative
Plasmodium falciparum PF3D7_0623700   ATP-dependent RNA helicase SUV3, putative
Plasmodium knowlesi PKNH_1126500   ATP-dependent RNA helicase SUV3, putative
Plasmodium vivax PVX_114300   ATP-dependent DEAD box helicase, putative
Plasmodium yoelii PY04199   Helicase conserved C-terminal domain, putative
Saccharomyces cerevisiae YPL029W   Suv3p
Trypanosoma brucei gambiense Tbg972.4.1910   RNA helicase, putative,mitochondrial, putative
Trypanosoma brucei Tb927.4.1990   ATP-dependent RNA and DNA helicase, mitochondrial, putative
Trypanosoma congolense TcIL3000_4_1730   ATP-dependent RNA and DNA helicase, mitochondrial, putative
Trypanosoma cruzi TcCLB.510877.70   ATP-dependent RNA and DNA helicase, mitochondrial, putative
Toxoplasma gondii TGME49_232530   hypothetical protein
Theileria parva TP01_1165   ATP-dependent DEAD box helicase, putative

Essentiality

Tb927.4.1990 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.1990 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.1990 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.1990 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.1990 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C08F8.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_C08F8.2 Caenorhabditis elegans larval arrest wormbase
CELE_C08F8.2 Caenorhabditis elegans slow growth wormbase
PBANKA_1122600 Plasmodium berghei Essential plasmo
TGME49_232530 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens suppressor of var1, 3-like 1 (S. cerevisiae) Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0054 1 1
0.0053 1 1
0.0051 1 1
0.0053 1 1
0.0057 0.5121 0.5
0.0056 0.7467 0
0.0053 1 0.5
0.0055 1 1
0.0055 1 1
0.0057 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.4.1990 (Trypanosoma brucei), ATP-dependent RNA and DNA helicase, mitochondrial, putative
Title for this comment
Comment