Detailed view for Tb927.8.5440

Basic information

TDR Targets ID: 14500
Trypanosoma brucei, flagellar calcium-binding 24 kDa protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.4958 | Length (AA): 218 | MW (Da): 24253 | Paralog Number: 5

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13202   EF hand
PF13499   EF-hand domain pair

Gene Ontology

Mouse over links to read term descriptions.
GO:0019861   flagellum  
GO:0009288   flagellin-based flagellum  
GO:0005509   calcium ion binding  
GO:0001539   ciliary or flagellar motility  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

PDB tag
  • 2LVV:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_133600)

Species Accession Gene Product
Leishmania braziliensis LbrM.16.0930   flagellar calcium-binding protein, putative
Leishmania donovani LdBPK_160930.1   flagellar calcium-binding protein, putative
Leishmania infantum LinJ.16.0930   flagellar calcium-binding protein, putative
Leishmania mexicana LmxM.16.0910   flagellar calcium-binding protein, putative
Leishmania mexicana LmxM.16.0920   flagellar calcium-binding protein, putative
Trypanosoma brucei gambiense Tbg972.8.5410   flagellar calcium-binding protein TB-24, putative
Trypanosoma brucei gambiense Tbg972.8.5430   flagellar calcium-binding protein Tb-17
Trypanosoma brucei Tb927.8.5460   Flagellar calcium-binding 44 kDa protein
Trypanosoma brucei Tb427.08.5465   24 kDa calcimedin
Trypanosoma brucei Tb11.v5.0798   flagellar calcium-binding protein
Trypanosoma brucei Tb11.v5.0797   flagellar calcium-binding protein
Trypanosoma brucei Tb927.8.5440   flagellar calcium-binding 24 kDa protein
Trypanosoma brucei Tb927.8.5470   flagellar calcium-binding 24 kDa protein
Trypanosoma congolense TcIL3000_0_43830   Flagellar calcium-binding protein 17, putative
Trypanosoma congolense TcIL3000_0_43820   Flagellar calcium-binding protein 24, putative
Trypanosoma congolense TcIL3000_8_5250   Flagellar calcium-binding protein 24, putative
Trypanosoma cruzi TcCLB.506749.20   flagellar calcium-binding 24 kDa protein
Trypanosoma cruzi TcCLB.509391.30   flagellar calcium-binding 24 kDa protein
Trypanosoma cruzi TcCLB.507891.38   flagellar calcium-binding 24 kDa protein
Trypanosoma cruzi TcCLB.507491.151   flagellar calcium-binding 24 kDa protein
Trypanosoma cruzi TcCLB.509391.10   flagellar calcium-binding 24 kDa protein
Trypanosoma cruzi TcCLB.507891.47   flagellar calcium-binding 24 kDa protein
Trypanosoma cruzi TcCLB.507891.29   flagellar calcium-binding 24 kDa protein
Trypanosoma cruzi TcCLB.509391.20   flagellar calcium-binding 24 kDa protein

Essentiality

Tb927.8.5440 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5460 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5460 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.5460 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.5460 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.5440 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5440 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.5440 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.5440 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.5470 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5470 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.5470 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.5470 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bos taurus Calmodulin 149 aa 25.0% 148 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tbru018108AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru018108; Recombinant protein: full-length; Source: T brucei; ;

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.8.5440 (Trypanosoma brucei), flagellar calcium-binding 24 kDa protein
Title for this comment
Comment