Detailed view for Tb927.8.4450

Basic information

TDR Targets ID: 14676
Trypanosoma brucei, RNA-binding protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.3421 | Length (AA): 428 | MW (Da): 47214 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00076   RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0003723   RNA binding  
GO:0003676   nucleic acid binding  
GO:0000166   nucleotide binding  
GO:0006396   RNA processing  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128388)

Species Accession Gene Product
Arabidopsis thaliana AT5G04600   RNA recognition motif-containing protein
Babesia bovis BBOV_IV003970   RNA recognition motif containing protein
Brugia malayi Bm1_45185   putative RNA binding protein
Candida albicans CaO19.7050   RRM-containing orf similar to SPCC1827.05c and to NOP15
Candida albicans CaO19_7050   hypothetical protein
Caenorhabditis elegans CELE_T04A8.6   Protein T04A8.6
Cryptosporidium hominis Chro.70191   mki67 (FHA domain) interacting nucleolar phosphoprotein (human) - like
Cryptosporidium parvum cgd7_1620   nop15p/nopp34; nucleolar protein with 1 RRM domain
Dictyostelium discoideum DDB_G0275885   hypothetical protein
Drosophila melanogaster Dmel_CG6937   CG6937 gene product from transcript CG6937-RA
Entamoeba histolytica EHI_068680   nucleolar phosphoprotein Nopp34, putative
Echinococcus multilocularis EmuJ_000452900   expressed protein
Giardia lamblia GL50803_6054   RNA binding protein, putative
Homo sapiens ENSG00000155438   nucleolar protein interacting with the FHA domain of MKI67
Leishmania braziliensis LbrM.10.1130   RNA-binding protein-like protein
Leishmania donovani LdBPK_101110.1   RNA-binding protein-like protein
Leishmania infantum LinJ.10.1110   RNA-binding protein-like protein
Leishmania major LmjF.10.1030   RNA-binding protein-like protein
Leishmania mexicana LmxM.10.1030   RNA-binding protein-like protein
Loa Loa (eye worm) LOAG_00920   hypothetical protein
Mus musculus ENSMUSG00000026377   nucleolar protein interacting with the FHA domain of MKI67
Neospora caninum NCLIV_052320   RNA binding protein, putative
Oryza sativa 4345558   Os08g0412200
Saccharomyces cerevisiae YNL110C   Nop15p
Schistosoma mansoni Smp_078520   nucleolar phosphoprotein
Trypanosoma brucei gambiense Tbg972.8.4210   RNA-binding protein, putative
Trypanosoma brucei Tb927.8.4450   RNA-binding protein, putative
Trypanosoma congolense TcIL3000_8_4220   RNA-binding protein, putative
Trypanosoma cruzi TcCLB.509569.120   RNA-binding protein, putative
Trypanosoma cruzi TcCLB.508989.30   RNA-binding protein, putative
Toxoplasma gondii TGME49_215400   RNA recognition motif-containing protein
Theileria parva TP01_1032   RNA-binding protein, putative
Trichomonas vaginalis TVAG_139660   conserved hypothetical protein
Trichomonas vaginalis TVAG_286120   conserved hypothetical protein

Essentiality

Tb927.8.4450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.4450 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.4450 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.4450 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.4450 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T04A8.6 Caenorhabditis elegans larval arrest wormbase
CELE_T04A8.6 Caenorhabditis elegans slow growth wormbase
CELE_T04A8.6 Caenorhabditis elegans sterile wormbase
YNL110C Saccharomyces cerevisiae inviable yeastgenome
TGME49_215400 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.8.4450 (Trypanosoma brucei), RNA-binding protein, putative
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