Detailed view for Tb927.11.12450

Basic information

TDR Targets ID: 14695
Trypanosoma brucei, Bardet-Biedl syndrome 8 protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5506 | Length (AA): 568 | MW (Da): 62629 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07719   Tetratricopeptide repeat
PF13181   Tetratricopeptide repeat
PF13432   Tetratricopeptide repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:1905515   GO:non-motile cilium assembly  

GO:0034464   GO:BBSome  

GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0003674   molecular_function  
GO:0005515   protein binding  
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
21 109 4cgw (A) 289 376 10.00 0.0038 0.03 0.24089 -0.58
21 116 2vyi (A) 98 192 7.00 0.096 0.01 0.203214 -0.4
248 555 4buj (B) 956 1297 14.00 0 1 0.592354 0.53
262 515 4i1a (B) 88 369 13.00 0 1 0.503283 0.37
389 445 2c2l (A) 71 127 37.00 0.24 1 0.568752 -0.08
442 499 5djs (A) 1 58 26.00 0 0.49 0.491213 -1.07
451 509 2vsy (A) 24 82 19.00 0 0.58 0.376973 -0.52
455 547 5fzs (A) 8 100 26.00 0.0016 0.89 0.576132 -0.73
480 552 3nf1 (A) 293 378 37.00 0.67 1 0.479921 -0.61
483 565 5mgx (E) 271 350 28.00 0.036 0.96 0.573527 -0.91
501 565 3ieg (A) 323 387 12.00 0 0.56 0.328537 -0.65

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130245)

Species Accession Gene Product
Caenorhabditis elegans CELE_T25F10.5   Protein BBS-8
Drosophila melanogaster Dmel_CG13691   Bardet-Biedl syndrome 8 ortholog (H. sapiens)
Giardia lamblia GL50803_8508   Basal body protein
Homo sapiens ENSG00000165533   tetratricopeptide repeat domain 8
Leishmania braziliensis LbrM.09.0450   hypothetical tetratricopeptide repeat protein
Leishmania donovani LdBPK_090490.1   Bardet-Biedl syndrome 8 protein
Leishmania infantum LinJ.09.0490   hypothetical tetratricopeptide repeat protein
Leishmania major LmjF.09.0440   hypothetical tetratricopeptide repeat protein
Leishmania mexicana LmxM.09.0440   hypothetical tetratricopeptide repeat protein
Loa Loa (eye worm) LOAG_08914   hypothetical protein
Loa Loa (eye worm) LOAG_01237   hypothetical protein
Mus musculus ENSMUSG00000021013   tetratricopeptide repeat domain 8
Schistosoma japonicum Sjp_0113970   Tetratricopeptide repeat protein 8, putative
Schistosoma mansoni Smp_160860   tetratricopeptide repeat protein 8 tpr8
Schmidtea mediterranea mk4.006700.02  
Schmidtea mediterranea mk4.002757.00   Tetratricopeptide repeat protein 8
Trypanosoma brucei gambiense Tbg972.11.13920   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.12450   Bardet-Biedl syndrome 8 protein
Trypanosoma congolense TcIL3000_0_24890   Bardet-Biedl syndrome 8 protein
Trypanosoma congolense TcIL3000_0_20320   Tetratricopeptide repeat/TPR repeat, putative
Trypanosoma cruzi TcCLB.503657.20   Bardet-Biedl syndrome 8 protein
Trypanosoma cruzi TcCLB.511127.360   tetratricopeptide repeat protein 8
Trichomonas vaginalis TVAG_337680   tetratricopeptide repeat protein 8, tpr8, putative

Essentiality

Tb927.11.12450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.4290 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.4290 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.4290 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.4290 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.12450 (Trypanosoma brucei), Bardet-Biedl syndrome 8 protein
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