Detailed view for Tb927.7.4400

Basic information

TDR Targets ID: 14755
Trypanosoma brucei, inositol hexakisphosphate kinase
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.6299 | Length (AA): 756 | MW (Da): 82630 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03770   Inositol polyphosphate kinase

Gene Ontology

Mouse over links to read term descriptions.
GO:0032958   inositol phosphate biosynthetic process  
GO:0016301   kinase activity  
GO:0008440   inositol trisphosphate 3-kinase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
427 482 2pne (A) 10 64 35.00 0.77 0.05 0.339174 0.74
508 756 2aqx (A) 650 933 29.00 0.000038 0.98 0.382465 0.39
525 756 1w2d (A) 197 456 22.00 0 1 0.363978 -0.04
535 755 2a98 (A) 431 677 23.00 0 1 0.364428 -0.19
578 755 2a98 (A) 470 677 29.00 0.049 0.93 0.32355 -0.21

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128105)

Species Accession Gene Product
Brugia malayi Bm1_41900   Inositol polyphosphate kinase family protein
Candida albicans CaO19.8622   similar to InositolP6 kinase
Candida albicans CaO19.1007   similar to InositolP6 kinase
Caenorhabditis elegans CELE_F30A10.3   Protein F30A10.3
Dictyostelium discoideum DDB_G0278739   inositol phosphate kinase
Dictyostelium discoideum DDB_G0283863   hypothetical protein
Drosophila melanogaster Dmel_CG10082   CG10082 gene product from transcript CG10082-RA
Entamoeba histolytica EHI_051050   inositol hexaphosphate kinase, putative
Entamoeba histolytica EHI_106120   inositol hexakisphosphate kinase, putative
Homo sapiens ENSG00000161896   inositol hexakisphosphate kinase 3
Homo sapiens ENSG00000176095   inositol hexakisphosphate kinase 1
Leishmania braziliensis LbrM.14.0340   inositol polyphosphate kinase-like protein, putative
Leishmania donovani LdBPK_140340.1   inositol polyphosphate kinase-like protein, putative
Leishmania infantum LinJ.14.0340   inositol polyphosphate kinase-like protein, putative
Leishmania major LmjF.14.0340   inositol polyphosphate kinase-like protein, putative
Leishmania mexicana LmxM.14.0340   inositol polyphosphate kinase-like protein, putative
Loa Loa (eye worm) LOAG_03580   hypothetical protein
Loa Loa (eye worm) LOAG_11012   inositol polyphosphate kinase
Mus musculus ENSMUSG00000032594   inositol hexaphosphate kinase 1
Mus musculus ENSMUSG00000024210   inositol hexaphosphate kinase 3
Neospora caninum NCLIV_002970   hypothetical protein
Onchocerca volvulus OVOC1377  
Plasmodium berghei PBANKA_1414600   inositol polyphosphate kinase, putative
Plasmodium falciparum PF3D7_1316100   inositol polyphosphate kinase, putative
Plasmodium knowlesi PKNH_1416800   inositol polyphosphate kinase, putative
Plasmodium vivax PVX_122630   inositol polyphosphate kinase, putative
Plasmodium yoelii PY06556   inositol hexakisphosphate kinase
Saccharomyces cerevisiae YDR017C   inositol polyphosphate kinase KCS1
Trypanosoma brucei gambiense Tbg972.7.4970   inositol polyphosphate kinase-like protein, putative
Trypanosoma brucei Tb927.7.4400   inositol hexakisphosphate kinase
Trypanosoma congolense TcIL3000_7_3620   inositol polyphosphate kinase-like protein, putative
Trypanosoma cruzi TcCLB.506985.60   inositol polyphosphate kinase-like protein, putative
Trypanosoma cruzi TcCLB.504213.90   inositol polyphosphate kinase-like protein, putative
Toxoplasma gondii TGME49_208070   inositol polyphosphate kinase
Trichomonas vaginalis TVAG_476200   inositol polyphosphate kinase, putative
Trichomonas vaginalis TVAG_410270   transcription factor, putative
Trichomonas vaginalis TVAG_574700   kcs1 protein, putative
Trichomonas vaginalis TVAG_175750   kcs1 protein, putative

Essentiality

Tb927.7.4400 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.4400 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.4400 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.4400 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.4400 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1414600 Plasmodium berghei Dispensable plasmo
TGME49_208070 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.7.4400 (Trypanosoma brucei), inositol hexakisphosphate kinase
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