Detailed view for Tb927.4.4740

Basic information

TDR Targets ID: 14774
Trypanosoma brucei, Sphingosine N-acyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.7448 | Length (AA): 400 | MW (Da): 45289 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 5

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03798   TLC domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
66 308 1rzh (L) 18 271 14.00 0.86 0.05 0.4697 1.83

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126838)

Species Accession Gene Product
Arabidopsis thaliana AT1G26200   TRAM, LAG1 and CLN8 (TLC) lipid-sensing domain containing protein
Arabidopsis thaliana AT3G19260   LAG1 longevity assurance-like 2
Arabidopsis thaliana AT3G25540   LAG1 longevity assurance-1
Arabidopsis thaliana AT1G13580   ceramide synthase LAG1
Babesia bovis BBOV_III005530   hypothetical protein
Brugia malayi Bm1_06625   homolog of yeast longevity gene protein 2
Candida albicans CaO19.7354   Longevity-assurance gene 1 cognate (LAG1 cognate)
Caenorhabditis elegans CELE_K02G10.6   Protein HYL-2
Caenorhabditis elegans CELE_C09G4.1   Protein HYL-1
Dictyostelium discoideum DDB_G0282607   ceramide synthase
Drosophila melanogaster Dmel_CG3576   CG3576 gene product from transcript CG3576-RA
Echinococcus granulosus EgrG_001184400   lag1 longevity assurance 5
Entamoeba histolytica EHI_139080   longevity-assurance family protein
Entamoeba histolytica EHI_151290   longevity-assurance family protein
Echinococcus multilocularis EmuJ_001184400   lag1 longevity assurance 5
Giardia lamblia GL50803_5939   Hypothetical protein
Homo sapiens ENSG00000172292   ceramide synthase 6
Homo sapiens ENSG00000090661   ceramide synthase 4
Homo sapiens ENSG00000139624   ceramide synthase 5
Homo sapiens ENSG00000143418   ceramide synthase 2
Leishmania braziliensis LbrM.31.2010   dihydroceramide synthase, putative
Leishmania donovani LdBPK_311800.1   Sphingosine N-acyltransferase, putative
Leishmania infantum LinJ.31.1800   dihydroceramide synthase, putative
Leishmania major LmjF.31.1780   dihydroceramide synthase, putative
Leishmania mexicana LmxM.30.1780   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_08632   hypothetical protein
Mus musculus ENSMUSG00000027035   ceramide synthase 6
Mus musculus ENSMUSG00000008206   ceramide synthase 4
Mus musculus ENSMUSG00000023021   ceramide synthase 5
Mus musculus ENSMUSG00000015714   ceramide synthase 2
Neospora caninum NCLIV_058920   hypothetical protein
Oryza sativa 4332339   Os03g0264000
Oryza sativa 4330600   Os02g0728300
Oryza sativa 4329785   Os02g0581300
Oryza sativa 9268169   Os09g0372700
Plasmodium berghei PBANKA_1038300   translocation associated membrane protein, putative
Plasmodium falciparum PF3D7_1403700   translocation associated membrane protein, putative
Plasmodium knowlesi PKNH_1354400   translocation associated membrane protein, putative
Plasmodium vivax PVX_086250   hypothetical protein, conserved
Plasmodium yoelii PY00812   tumor metastasis-suppressor
Schistosoma japonicum Sjp_0302590   LAG1 longevity assurance homolog 5, putative
Schistosoma mansoni Smp_042440   dihydroceramide synthase
Schmidtea mediterranea mk4.002284.03  
Schmidtea mediterranea mk4.009230.00  
Schmidtea mediterranea mk4.009105.00  
Trypanosoma brucei gambiense Tbg972.8.7980   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.4.4900   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.7730   Sphingosine N-acyltransferase, putative
Trypanosoma brucei Tb927.4.4740   Sphingosine N-acyltransferase, putative
Trypanosoma congolense TcIL3000_0_57130   Sphingosine N-acyltransferase, putative
Trypanosoma congolense TcIL3000_0_38500   Sphingosine N-acyltransferase, putative
Trypanosoma congolense TcIL3000_0_52910   Sphingosine N-acyltransferase, putative
Trypanosoma congolense TcIL3000_0_02670   Sphingosine N-acyltransferase, putative
Trypanosoma cruzi TcCLB.510087.30   Sphingosine N-acyltransferase, putative
Trypanosoma cruzi TcCLB.507395.10   Sphingosine N-acyltransferase, putative
Toxoplasma gondii TGME49_316450   longevity-assurance protein (LAG1) domain-containing protein
Theileria parva TP02_0395   hypothetical protein, conserved
Trichomonas vaginalis TVAG_254300   longevity assurance factor, putative

Essentiality

Tb927.4.4740 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.4740 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.4740 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.4740 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.4740 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.7730 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.7730 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.7730 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.7730 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1038300 Plasmodium berghei Dispensable plasmo
TGME49_316450 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.4.4740 (Trypanosoma brucei), Sphingosine N-acyltransferase, putative
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