Detailed view for Tb927.8.3130

Basic information

TDR Targets ID: 14845
Trypanosoma brucei, S-adenosyl-methyltransferase mraW-like protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.1076 | Length (AA): 474 | MW (Da): 53240 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01795   MraW methylase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0008150   biological_process  
GO:0008168   methyltransferase activity  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
110 404 1wg8 (A) 6 284 27.00 0 1 0.929363 -0.23
130 404 1wg8 (A) 26 284 32.00 0 1 0.880469 0
130 209 1l3i (A) 37 112 20.00 0 0.62 0.364076 -0.39
130 225 3ntv (A) 75 170 15.00 0 0.9 0.451832 -0.56

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127913)

Species Accession Gene Product
Arabidopsis thaliana AT5G10910   mraW methylase family protein
Babesia bovis BBOV_IV000910   S-adenosyl methyltransferase, putative
Chlamydia trachomatis CT_272   ribosomal RNA small subunit methyltransferase H
Dictyostelium discoideum DDB_G0284343   hypothetical protein
Drosophila melanogaster Dmel_CG14683   CG14683 gene product from transcript CG14683-RA
Escherichia coli b0082   16S rRNA m(4)C1402 methyltransferase, SAM-dependent
Echinococcus granulosus EgrG_000877800   S adenosyl L methionine dependent
Echinococcus multilocularis EmuJ_000877800   S adenosyl L methionine dependent
Homo sapiens ENSG00000169519   methyltransferase like 15
Leishmania braziliensis LbrM.23.1300   S-adenosyl-methyltransferase mraW-like protein
Leishmania donovani LdBPK_231440.1   S-adenosyl-methyltransferase mraW-like protein
Leishmania infantum LinJ.23.1440   S-adenosyl-methyltransferase mraW-like protein
Leishmania major LmjF.23.1200   S-adenosyl-methyltransferase mraW-like protein
Leishmania mexicana LmxM.23.1200   S-adenosyl-methyltransferase mraW-like protein
Mycobacterium leprae ML0906   conserved hypothetical protein
Mus musculus ENSMUSG00000057234   methyltransferase like 15
Mycobacterium tuberculosis Rv2165c   Conserved protein
Mycobacterium ulcerans MUL_3510   S-adenosyl-methyltransferase MraW
Neospora caninum NCLIV_052430   S-adenosyl-methyltransferase mraW, putative
Oryza sativa 4328239   Os02g0137600
Plasmodium berghei PBANKA_1451300   S-adenosyl-methyltransferase, putative
Plasmodium falciparum PF3D7_1236700   S-adenosyl-methyltransferase, putative
Plasmodium knowlesi PKNH_1456400   S-adenosyl-methyltransferase, putative
Plasmodium vivax PVX_100785   S-adenosyl-methyltransferase mraW, putative
Plasmodium yoelii PY05542   s-adenosyl-methyltransferase mraw-related
Schistosoma japonicum Sjp_0074080   ko:K03438 S-adenosyl-methyltransferase, putative
Schistosoma japonicum Sjp_0074180   ko:K03438 S-adenosyl-methyltransferase, putative
Schistosoma mansoni Smp_053120   S-adenosyl-methyltransferase mraW
Trypanosoma brucei gambiense Tbg972.8.2860   S-adenosyl-methyltransferase mraW-like protein, putative
Trypanosoma brucei Tb927.8.3130   S-adenosyl-methyltransferase mraW-like protein, putative
Trypanosoma congolense TcIL3000_8_3130   S-adenosyl-methyltransferase mraW-like protein, putative
Trypanosoma cruzi TcCLB.510515.130   S-adenosyl-methyltransferase mraW-like protein, putative
Trypanosoma cruzi TcCLB.504003.70   S-adenosyl-methyltransferase mraW-like protein, putative
Toxoplasma gondii TGME49_215520   hypothetical protein
Treponema pallidum TP0384   hypothetical protein
Theileria parva TP01_0113   S-adenosyl-methyltransferase mraW, putative
Wolbachia endosymbiont of Brugia malayi Wbm0107   S-adenosyl-methyltransferase MraW

Essentiality

Tb927.8.3130 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.3130 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.3130 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.3130 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.3130 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0082 Escherichia coli non-essential goodall
TGME49_215520 Toxoplasma gondii Essentiality uncertain sidik
TGME49_215520 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

33 literature references were collected for this gene.

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Gene identifier Tb927.8.3130 (Trypanosoma brucei), S-adenosyl-methyltransferase mraW-like protein, putative
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