Detailed view for Tb927.4.2860

Basic information

TDR Targets ID: 14909
Trypanosoma brucei, EAP30/Vps36 family, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.2631 | Length (AA): 347 | MW (Da): 38055 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04157   EAP30/Vps36 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0071985   GO:multivesicular body sorting pathway  

GO:0000814   ESCRT II complex  

Metabolic Pathways

Endocytosis (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128498)

Species Accession Gene Product
Arabidopsis thaliana AT4G27040   vacuolar protein sorting-associated protein 22
Babesia bovis BBOV_II005030   hypothetical protein
Brugia malayi Bm1_05145   EAP30 subunit of ELL complex
Candida albicans CaO19.6296   involved in glucose derepression
Candida albicans CaO19.13675   involved in glucose derepression
Caenorhabditis elegans CELE_C27F2.5   Protein VPS-22
Dictyostelium discoideum DDB_G0283203   EAP30 family protein
Drosophila melanogaster Dmel_CG6637   larsen
Echinococcus granulosus EgrG_000441300   vacuolar sorting protein SNF8
Entamoeba histolytica EHI_131120   ELL complex EAP30 subunit, putative
Echinococcus multilocularis EmuJ_000441300   vacuolar sorting protein SNF8
Giardia lamblia GL50803_90710   Hypothetical protein
Homo sapiens 11267   SNF8, ESCRT-II complex subunit
Leishmania braziliensis LbrM.14.1240   hypothetical protein, conserved
Leishmania donovani LdBPK_341570.1   EAP30/Vps36 family, putative
Leishmania infantum LinJ.34.1570   hypothetical protein, conserved
Leishmania major LmjF.34.1470   hypothetical protein, conserved
Leishmania mexicana LmxM.33.1470   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_08144   hypothetical protein
Mus musculus ENSMUSG00000006058   SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Oryza sativa 9269587   Os09g0529700
Saccharomyces cerevisiae YPL002C   ESCRT-II subunit protein SNF8
Schistosoma japonicum Sjp_0005470   Vacuolar-sorting protein SNF8, putative
Schistosoma mansoni Smp_130400   hypothetical protein
Schmidtea mediterranea mk4.005180.00   Vacuolar-sorting protein SNF8
Trypanosoma brucei gambiense Tbg972.4.2840   hypothetical protein, conserved
Trypanosoma brucei Tb927.4.2860   EAP30/Vps36 family, putative
Trypanosoma congolense TcIL3000_4_2760   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506263.40   EAP30/Vps36 family, putative
Theileria parva TP04_0045   hypothetical protein
Trichomonas vaginalis TVAG_107410   spbc651.05C protein, putative

Essentiality

Tb927.4.2860 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2860 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.2860 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.2860 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.2860 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.4.2860 (Trypanosoma brucei), EAP30/Vps36 family, putative
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