Detailed view for Tb927.10.7570

Basic information

TDR Targets ID: 14971
Trypanosoma brucei, dihydrolipoamide acetyltransferase E2 subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.3267 | Length (AA): 451 | MW (Da): 48107 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00198   2-oxoacid dehydrogenases acyltransferase (catalytic domain)
PF00364   Biotin-requiring enzyme
PF02817   e3 binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016746   transferase activity, transferring acyl groups  
GO:0008415   acyltransferase activity  
GO:0005515   protein binding  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128029)

Species Accession Gene Product
Arabidopsis thaliana AT3G13930   dihydrolipoamide acetyltransferase, long form protein
Arabidopsis thaliana AT1G54220   dihydrolipoyllysine-residue acetyltransferase component 3 of pyruvate dehydrogenase complex
Brugia malayi Bm1_10875   dihydrolipoamide S-acetyltransferase
Candida albicans CaO19.6561   dihydrolipoamide acetyltransferase
Candida albicans CaO19.13914   dihydrolipoamide acetyltransferase
Candida albicans CaO19_6561   hypothetical protein
Caenorhabditis elegans CELE_F23B12.5   Protein DLAT-1
Chlamydia trachomatis CT_247   dihydrolipoamide acetyltransferase
Dictyostelium discoideum DDB_G0277847   dihydrolipoamide acetyltransferase
Drosophila melanogaster Dmel_CG5261   CG5261 gene product from transcript CG5261-RB
Echinococcus granulosus EgrG_000321550   Biotin lipoyl attachment
Echinococcus multilocularis EmuJ_000321550   Biotin lipoyl attachment
Homo sapiens ENSG00000150768   dihydrolipoamide S-acetyltransferase
Leishmania braziliensis LbrM.35.2870   dihydrolipoamide acetyltransferase precursor, putative
Leishmania donovani LdBPK_362790.1   dihydrolipoamide acetyltransferase precursor, putative
Leishmania infantum LinJ.36.2790   dihydrolipoamide acetyltransferase precursor, putative
Leishmania major LmjF.36.2660   dihydrolipoamide acetyltransferase precursor, putative
Leishmania mexicana LmxM.36.2660   dihydrolipoamide acetyltransferase precursor, putative
Loa Loa (eye worm) LOAG_16098   hypothetical protein
Loa Loa (eye worm) LOAG_10883   dihydrolipoamide S-acetyltransferase
Loa Loa (eye worm) LOAG_15745   hypothetical protein
Mus musculus ENSMUSG00000000168   dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)
Oryza sativa 4339859   Os06g0105400
Oryza sativa 4343003   Os07g0410100
Oryza sativa 4328010   Os02g0105200
Saccharomyces cerevisiae YNL071W   dihydrolipoyllysine-residue acetyltransferase
Schistosoma japonicum Sjp_0216460   ko:K00627 pyruvate dehydrogenase E2 component (dihydrolipoamide [EC2.3.1.12], putative
Schistosoma japonicum Sjp_0134970   Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial precursor, putative
Schistosoma mansoni Smp_194460   dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase
Schmidtea mediterranea mk4.000019.10   Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Schmidtea mediterranea mk4.065814.00  
Trypanosoma brucei gambiense Tbg972.10.9270   dihydrolipoamide acetyltransferase precursor, putative
Trypanosoma brucei Tb927.10.7570   dihydrolipoamide acetyltransferase E2 subunit, putative
Trypanosoma brucei Tb11.v5.0774   dihydrolipoamide acetyltransferase precursor, putative
Trypanosoma congolense TcIL3000_10_6510   dihydrolipoamide acetyltransferase E2 subunit, putative
Trypanosoma cruzi TcCLB.509717.20   dihydrolipoamide acetyltransferase precursor, putative
Trypanosoma cruzi TcCLB.510105.170   dihydrolipoamide acetyltransferase precursor, putative
Wolbachia endosymbiont of Brugia malayi Wbm0747   branched-chain alpha-keto acid dehydrogenase subunit E2

Essentiality

Tb927.10.7570 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.7570 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.7570 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.7570 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.7570 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F23B12.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_F23B12.5 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.7570 (Trypanosoma brucei), dihydrolipoamide acetyltransferase E2 subunit, putative
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