Detailed view for Tb927.8.2930

Basic information

TDR Targets ID: 15034
Trypanosoma brucei, mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.1548 | Length (AA): 597 | MW (Da): 66434 | Paralog Number: 3

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01532   Glycosyl hydrolase family 47

Gene Ontology

Mouse over links to read term descriptions.
GO:0004571   mannosyl-oligosaccharide 1,2-alpha-mannosidase activity  
GO:0016020   membrane  
GO:0005509   calcium ion binding  
GO:0003824   catalytic activity  
GO:0006487   protein amino acid N-linked glycosylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127787)

Species Accession Gene Product
Arabidopsis thaliana AT5G43710   putative alpha-mannosidase I MNS4
Arabidopsis thaliana AT1G27520   putative alpha-mannosidase I MNS5
Brugia malayi Bm1_26630   Glycosyl hydrolase family 47 protein
Candida albicans CaO19.834   similar to alpha-mannosidase
Candida albicans CaO19.8454   similar to alpha-mannosidase
Caenorhabditis elegans CELE_F10C2.5   Protein F10C2.5
Caenorhabditis elegans CELE_C47E12.3   Protein C47E12.3
Dictyostelium discoideum DDB_G0277051   glycoside hydrolase family 47 protein
Drosophila melanogaster Dmel_CG3810   CG3810 gene product from transcript CG3810-RC
Echinococcus granulosus EgrG_001033900   er degradation enhancer mannosidase alpha
Echinococcus granulosus EgrG_001034000   er degradation enhancing alpha mannosidase
Echinococcus granulosus EgrG_000774050   er degradation enhancing alpha mannosidase
Echinococcus multilocularis EmuJ_001034000   er degradation enhancing alpha mannosidase er degradation enhancer mannosidase alpha
Echinococcus multilocularis EmuJ_000774050   er degradation enhancing alpha mannosidase
Echinococcus multilocularis EmuJ_001033900   ER degradation enhancer, mannosidase alpha
Homo sapiens ENSG00000134109   ER degradation enhancer, mannosidase alpha-like 1
Homo sapiens ENSG00000088298   ER degradation enhancer, mannosidase alpha-like 2
Loa Loa (eye worm) LOAG_01644   hypothetical protein
Loa Loa (eye worm) LOAG_01645   hypothetical protein
Loa Loa (eye worm) LOAG_04306   hypothetical protein
Loa Loa (eye worm) LOAG_08032   hypothetical protein
Mus musculus ENSMUSG00000038312   ER degradation enhancer, mannosidase alpha-like 2
Mus musculus ENSMUSG00000030104   ER degradation enhancer, mannosidase alpha-like 1
Oryza sativa 4327149   Os01g0773600
Oryza sativa 4330685   Os02g0741300
Saccharomyces cerevisiae YHR204W   alpha-1,2-mannosidase MNL1
Schistosoma japonicum Sjp_0020580   ko:K10085 ER degradation enhancer, mannosidase alpha-like 2, putative
Schistosoma japonicum Sjp_0122240   ER degradation-enhancing alpha-mannosidase-like 1, putative
Schistosoma japonicum Sjp_0072960   ko:K10084 ER degradation enhancer, mannosidase alpha-like 1, putative
Schistosoma japonicum Sjp_0072950   ER degradation-enhancing alpha-mannosidase-like 1, putative
Schistosoma mansoni Smp_143170   mannosyl-oligosaccharidealpha-12-mannosidase-r el ated
Schistosoma mansoni Smp_100120   er degradation-enhancing alpha-mannosidase-like
Schmidtea mediterranea mk4.000732.04   ER degradation-enhancing alpha-mannosidase-like protein 1
Schmidtea mediterranea mk4.000732.03   ER degradation-enhancing alpha-mannosidase-like protein 1
Schmidtea mediterranea mk4.000668.01  
Schmidtea mediterranea mk4.000668.02   ER degradation-enhancing alpha-mannosidase-like protein 2
Schmidtea mediterranea mk4.000732.07   ER degradation-enhancing alpha-mannosidase-like protein 1
Trypanosoma brucei gambiense Tbg972.8.2580  
Trypanosoma brucei gambiense Tbg972.8.2570   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma brucei gambiense Tbg972.8.2590   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma brucei gambiense Tbg972.8.2600   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma brucei Tb927.8.2910   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma brucei Tb927.8.2920   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma brucei Tb927.8.2940   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma brucei Tb927.8.2930   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma congolense TcIL3000_8_2840   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma congolense TcIL3000_8_2830   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma cruzi TcCLB.510513.10   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma cruzi TcCLB.507799.10   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma cruzi TcCLB.506345.120   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma cruzi TcCLB.508317.120   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma cruzi TcCLB.508317.80   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
Trypanosoma cruzi TcCLB.505267.50   mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative

Essentiality

Tb927.8.2930 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.2910 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.2910 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.2910 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.2910 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Glycine max Alpha-mannosidase 578 aa 32.5% 483 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Tb927.8.2930 (Trypanosoma brucei), mannosyl-oligosaccharide 1,2-alpha-mannosidase IB, putative
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