Detailed view for Tb427.04.1100

Basic information

TDR Targets ID: 15095
Trypanosoma brucei, ribosomal protein L21E (60S), putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 11.183 | Length (AA): 159 | MW (Da): 18040 | Paralog Number: 3

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01157   Ribosomal protein L21e

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

PDB tag
  • 3ZF7:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126766)

Species Accession Gene Product
Arabidopsis thaliana AT1G09690   60S ribosomal protein L21-1
Arabidopsis thaliana AT1G57860   translation protein SH3-like family protein
Arabidopsis thaliana AT1G57660   60S ribosomal protein L21-2
Arabidopsis thaliana AT1G09590   60S ribosomal protein L21-1
Babesia bovis BBOV_III010520   ribosomal protein L21, putative
Brugia malayi Bm1_47445   60S ribosomal protein L21
Candida albicans CaO19.840   similar to C terminus of S. cerevisiae RPL21A (YBR191W) and RPL21B (YPL079W) genes for cytosolic ribosomal protein large subunit
Candida albicans CaO19.8460   similar to C terminus of S. cerevisiae RPL21A (YBR191W) and RPL21B (YPL079W) genes for cytosolic ribosomal protein large subunit
Caenorhabditis elegans CELE_C14B9.7   Protein RPL-21
Cryptosporidium hominis Chro.60285   ribosomal protein L21
Cryptosporidium parvum cgd6_2460   60s ribosomal protein L21
Dictyostelium discoideum DDB_G0279387   S60 ribosomal protein L21
Drosophila melanogaster Dmel_CG12775   Ribosomal protein L21
Echinococcus granulosus EgrG_000582200   60S ribosomal protein L21
Entamoeba histolytica EHI_161180   60S ribosomal protein L21, putative
Entamoeba histolytica EHI_069110   60S ribosomal protein L21, putative
Entamoeba histolytica EHI_182920   60S ribosomal protein L21, putative
Echinococcus multilocularis EmuJ_000582200   60S ribosomal protein L21
Giardia lamblia GL50803_15520   Ribosomal protein L21
Homo sapiens ENSG00000122026   ribosomal protein L21
Leishmania braziliensis LbrM.16.0480   60S ribosomal protein L21, putative
Leishmania braziliensis LbrM.20.3240   60S ribosomal protein L21, putative
Leishmania donovani LdBPK_343440.1   60S ribosomal protein L21, putative
Leishmania infantum LinJ.16.0470   60S ribosomal protein L21, putative
Leishmania infantum LinJ.34.3440   60S ribosomal protein L21, putative
Leishmania major LmjF.16.0460   60S ribosomal protein L21, putative
Leishmania major LmjF.34.3650   60S ribosomal protein L21, putative
Leishmania mexicana LmxM.16.0460   60S ribosomal protein L21, putative
Leishmania mexicana LmxM.33.3650   60S ribosomal protein L21, putative
Loa Loa (eye worm) LOAG_04391   60S ribosomal protein L21
Mus musculus ENSMUSG00000041453   ribosomal protein L21
Mus musculus 636187   predicted gene, 20764
Mus musculus 102643293   60S ribosomal protein L21-like
Mus musculus 102641365   60S ribosomal protein L21-like
Neospora caninum NCLIV_062950   50S ribosomal protein L21e, related
Oryza sativa 9266412   Os03g0141000
Oryza sativa 4348839   Os10g0465800
Plasmodium berghei PBANKA_1018600   60S ribosomal protein L21, putative
Plasmodium falciparum PF3D7_1426000   60S ribosomal protein L21
Plasmodium knowlesi PKNH_1332700   60S ribosomal protein L21, putative
Plasmodium vivax PVX_085225   60S ribosomal protein L21, putative
Plasmodium yoelii PY05142   Ribosomal protein L21e, putative
Saccharomyces cerevisiae YPL079W   ribosomal 60S subunit protein L21B
Saccharomyces cerevisiae YBR191W   ribosomal 60S subunit protein L21A
Schistosoma japonicum Sjp_0069130   ko:K02889 large subunit ribosomal protein L21e, putative
Schistosoma mansoni Smp_022560.2   60S ribosomal protein L21
Schistosoma mansoni Smp_022560.1   60S ribosomal protein L21
Schmidtea mediterranea mk4.000937.01   60S ribosomal protein L21
Trypanosoma brucei gambiense Tbg972.11.660   ribosomal protein L21E (60S), putative
Trypanosoma brucei gambiense Tbg972.4.930   ribosomal protein L21E (60S), putative
Trypanosoma brucei Tb927.4.1100   ribosomal protein L21E (60S), putative
Trypanosoma brucei Tb11.0880   chrXI additional, unordered contigs
Trypanosoma brucei Tb927.11.680   ribosomal protein L21E (60S), putative
Trypanosoma brucei Tb427.04.1100   ribosomal protein L21E (60S), putative
Trypanosoma congolense TcIL3000.11.670   ribosomal protein L21E (60S), putative
Trypanosoma cruzi TcCLB.507251.20   ribosomal protein L21E (60S), putative
Trypanosoma cruzi TcCLB.506405.149   ribosomal protein L21E (60S), putative
Toxoplasma gondii TGME49_245680   ribosomal protein RPL21
Theileria parva TP02_0089   60S ribosomal protein L21e, putative
Trichomonas vaginalis TVAG_277060   60S ribosomal protein L21, putative
Trichomonas vaginalis TVAG_141940   60S ribosomal protein L21, putative
Trichomonas vaginalis TVAG_119330   60S ribosomal protein L21, putative
Trichomonas vaginalis TVAG_446000   60S ribosomal protein L21, putative
Trichomonas vaginalis TVAG_231260   60S ribosomal protein L21, putative
Trichomonas vaginalis TVAG_231250   60S ribosomal protein L21, putative

Essentiality

Tb427.04.1100 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.50.0005 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.50.0005 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.50.0005 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.50.0005 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.4.1100 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.1100 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.1100 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.1100 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C14B9.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_C14B9.7 Caenorhabditis elegans larval arrest wormbase
CELE_C14B9.7 Caenorhabditis elegans slow growth wormbase
CELE_C14B9.7 Caenorhabditis elegans sterile wormbase
TGME49_245680 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb427.04.1100 (Trypanosoma brucei), ribosomal protein L21E (60S), putative
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