Detailed view for Tb927.4.2460

Basic information

TDR Targets ID: 15119
Trypanosoma brucei, kinatase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.8009 | Length (AA): 1286 | MW (Da): 142922 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain
PF00782   Dual specificity phosphatase, catalytic domain
PF13855   Leucine rich repeat

Gene Ontology

Mouse over links to read term descriptions.
GO:0016791   phosphoric monoester hydrolase activity  
GO:0008138   protein tyrosine/serine/threonine phosphatase activity  
GO:0005524   ATP binding  
GO:0005515   protein binding  
GO:0004725   protein tyrosine phosphatase activity  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0016311   dephosphorylation  
GO:0006470   protein amino acid dephosphorylation  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 14 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
119 720 4oli (A) 592 1177 16.00 0 1 0.338918 0.78
173 383 2zv7 (A) 284 489 27.00 0.0000025 1 0.410775 -0.26
223 384 4mvf (A) 162 317 38.00 0.0038 0.96 0.135672 0.37
314 384 5knj (A) 392 459 37.00 0.13 1 0.55391 -0.98
416 755 1kob (A) 24 353 15.00 0 0.91 0.363186 0.11
715 1060 4mna (A) 74 530 22.00 0.0000000022 0.8 0.310851 0.97
791 1075 4u08 (A) 135 405 25.00 0 1 0.338417 0.62
943 1030 3bz5 (A) 72 155 21.00 0 1 0.341229 -0.39
944 1047 4nkh (A) 281 373 39.00 0.0032 0.75 0.351571 0.55
952 1050 4r58 (A) 5 101 19.00 0 0.29 0.285783 -0.6
970 1039 2ft3 (A) 246 325 21.00 0 0.8 0.189232 -0.13
971 1049 2a0z (A) 36 114 38.00 0.16 0.91 0.463131 -0.1
1127 1265 2hcm (A) 11 149 29.00 0 1 0.645887 -1.76
1188 1266 2esb (A) 80 159 44.00 0.00000006 0.98 0.642131 -0.82

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_138464)

Species Accession Gene Product
Dictyostelium discoideum DDB_G0278445   leucine-rich repeat-containing protein
Dictyostelium discoideum DDB_G0269918   leucine-rich repeat-containing protein
Dictyostelium discoideum DDB_G0270688   leucine-rich repeat-containing protein
Entamoeba histolytica EHI_099820   leucine rich repeat and phosphatase domain containing protein
Leishmania braziliensis LbrM.20.1690   dual specificity protein phosphatase, putative
Leishmania donovani LdBPK_341950.1   kinatase, putative
Leishmania infantum LinJ.34.1950   dual specificity protein phosphatase, putative
Leishmania major LmjF.34.2190   dual specificity protein phosphatase, putative
Leishmania mexicana LmxM.33.2190   dual specificity protein phosphatase, putative
Trypanosoma brucei gambiense Tbg972.4.2420   dual specificity protein phosphatase, putative
Trypanosoma brucei Tb927.4.2460   kinatase, putative
Trypanosoma brucei Tb11.v5.0751   dual specificity protein phosphatase, putative
Trypanosoma congolense TcIL3000_4_2340   kinatase, putative
Trypanosoma cruzi TcCLB.510949.10   kinatase, putative
Trypanosoma cruzi TcCLB.506559.190   kinatase, putative

Essentiality

Tb927.4.2460 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2460 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.2460 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.2460 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.2460 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0029 0.5 0.5
0.0016 0.5 0.5
0.0069 0.3067 1
0.0008 0.5 0.5
0.0063 0.7244 0.2543
0.0056 1 0.5
0.0033 0.5 0.5
0.0081 0.5 0.5
0.0012 0.5 0.5
0.0036 0.5 0.5
0.0059 1 1
0.0022 0.5 0.5
0.0093 0.8828 0
0.0092 1 0.5
0.0039 0.9485 0.5
0.0007 0.5 0.5
0.0088 0.4477 0.5
0.0061 0.6883 0.5304
0.0027 1 0.5
0.0081 1 0.5
0.0042 0.5 0.5
0.0098 0.3242 0.2614
0.0064 0.3377 0
0.0032 0.5 0.5
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0004 0.5 0.5
0.0023 0.5 0.5
0.0012 0.5 0.5
0.0032 0.5 0.5
0.0062 0.6935 0
0.0067 0.5 0.5
0.0059 1 1
0.0007 0.5 0.5
0.0007 0.5 0.5
0.0003 0.5 0.5
0.0033 1 0.5
0.0059 1 1
0.0066 0.3101 0
0.0018 0.5 0.5
0.0039 0.5 0.5
0.0011 1 0.5
0.0091 1 0.5
0.0063 1 0.5
0.0039 0.5 0.5
0.0026 0.5 0.5
0.0037 1 0.5

Assayability

Assay information

  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 1 )

Reagent availability

No reagent availability information for this target.

Bibliographic References

18 literature references were collected for this gene.

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Gene identifier Tb927.4.2460 (Trypanosoma brucei), kinatase, putative
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