Detailed view for Tb927.6.620

Basic information

TDR Targets ID: 15207
Trypanosoma brucei, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6358 | Length (AA): 2728 | MW (Da): 301889 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF14874   Flagellar-associated PapD-like

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
135 238 2e6j (A) 8 111 18.00 0 0.75 0.298023 -0.51
377 468 2ys4 (A) 29 119 22.00 0 0.97 0.323624 -0.11
756 912 1fa2 (A) 2 152 28.00 0.42 0.23 0.129651 0.91
843 931 3qbt (B) 572 675 24.00 0 1 0.231525 -0.76
2299 2385 1z9l (A) 5 89 18.00 0 0.32 0.270792 -0.78
2309 2379 1wic (A) 24 94 23.00 0 0.5 0.391926 -1.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129916)

Species Accession Gene Product
Echinococcus granulosus EgrG_000510300   hypothetical protein
Echinococcus multilocularis EmuJ_000510300   hypothetical protein
Homo sapiens ENSG00000205081   chromosome X open reading frame 30
Homo sapiens ENSG00000165164   chromosome X open reading frame 22
Leishmania braziliensis LbrM.22.1530   hypothetical protein, conserved
Leishmania donovani LdBPK_221470.1   hypothetical protein, conserved
Leishmania infantum LinJ.22.1470   hypothetical protein, conserved
Leishmania major LmjF.22.1620   hypothetical protein, conserved
Leishmania mexicana LmxM.22.1620   hypothetical protein, conserved
Mus musculus ENSMUSG00000079517   predicted gene 8787
Mus musculus ENSMUSG00000073077   predicted gene 7173
Mus musculus 636082   predicted gene 16462
Schistosoma japonicum Sjp_0096670   Conserved hypothetical protein
Schistosoma japonicum Sjp_0020190   Conserved hypothetical protein
Schistosoma mansoni Smp_086000   hypothetical protein
Schmidtea mediterranea mk4.015531.02  
Schmidtea mediterranea mk4.010040.03  
Schmidtea mediterranea mk4.013290.01  
Schmidtea mediterranea mk4.015082.00  
Schmidtea mediterranea mk4.015082.04  
Schmidtea mediterranea mk4.017098.00  
Schmidtea mediterranea mk4.010040.02  
Schmidtea mediterranea mk4.015531.01  
Trypanosoma brucei gambiense Tbg972.6.310   hypothetical protein, conserved
Trypanosoma brucei Tb927.6.620   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_6_140   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511827.20   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510535.20   hypothetical protein, conserved
Trichomonas vaginalis TVAG_281170   conserved hypothetical protein
Trichomonas vaginalis TVAG_051730   conserved hypothetical protein
Trichomonas vaginalis TVAG_473440   conserved hypothetical protein
Trichomonas vaginalis TVAG_533970   conserved hypothetical protein
Trichomonas vaginalis TVAG_379040   conserved hypothetical protein
Trichomonas vaginalis TVAG_373180   conserved hypothetical protein
Trichomonas vaginalis TVAG_259310   conserved hypothetical protein

Essentiality

Tb927.6.620 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.620 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.620 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.6.620 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.620 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.6.620 (Trypanosoma brucei), hypothetical protein, conserved
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