Detailed view for Tb927.5.900

Basic information

TDR Targets ID: 15219
Trypanosoma brucei, oligosaccharyl transferase subunit, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.7968 | Length (AA): 821 | MW (Da): 92233 | Paralog Number: 4

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 11

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02516   Oligosaccharyl transferase STT3 subunit

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0004576   oligosaccharyl transferase activity  
GO:0006486   protein amino acid glycosylation  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126840)

Species Accession Gene Product
Arabidopsis thaliana AT5G19690   dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
Arabidopsis thaliana AT1G34130   dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Babesia bovis BBOV_II000220   oligosaccharyl transferase STT3 subunit, putative
Brugia malayi Bm1_06940   Oligosaccharyl transferase STT3 subunit homolog, putative
Brugia malayi Bm1_32435   ITM1 protein
Candida albicans CaO19.9053   subunit of oligosaccharyltransferase
Candida albicans CaO19.1478   subunit of oligosaccharyltransferase
Caenorhabditis elegans CELE_T12A2.2   Protein STT-3
Cryptosporidium hominis Chro.60239   oligosaccharyl transferase stt3 protein
Cryptosporidium parvum cgd6_2040   oligosaccharyl transferase STT3 protein
Dictyostelium discoideum DDB_G0285159   dolichyl-diphosphooligosaccharide-protein glycotransferase
Drosophila melanogaster Dmel_CG1518   CG1518 gene product from transcript CG1518-RC
Drosophila melanogaster Dmel_CG7748   Oligosaccharyl transferase 3
Echinococcus granulosus EgrG_000181500   oligosaccharyl transferase
Echinococcus granulosus EgrG_000999300   STT3 subunit of the oligosaccharyltransferase
Entamoeba histolytica EHI_011940   dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, putative
Entamoeba histolytica EHI_118880   Oligosaccharyl transferase, putative
Echinococcus multilocularis EmuJ_000181500   oligosaccharyl transferase
Echinococcus multilocularis EmuJ_000999300   STT3, subunit of the oligosaccharyltransferase
Giardia lamblia GL50803_137685   Hypothetical protein
Homo sapiens ENSG00000163527   STT3B, subunit of the oligosaccharyltransferase complex (catalytic)
Homo sapiens ENSG00000134910   STT3A, subunit of the oligosaccharyltransferase complex (catalytic)
Leishmania braziliensis LbrM.34.1060   oligosaccharyl transferase-like protein
Leishmania braziliensis LbrM.27.0430   oligosaccharyl transferase-like protein
Leishmania braziliensis LbrM.34.1070   oligosaccharyl transferase subunit, putative
Leishmania braziliensis LbrM.34.1050   oligosaccharyl transferase-like protein
Leishmania donovani LdBPK_351160.1   oligosaccharyl transferase-like protein
Leishmania infantum LinJ.35.1140   oligosaccharyl transferase-like protein
Leishmania infantum LinJ.35.1150   oligosaccharyl transferase-like protein
Leishmania infantum LinJ.35.1170   oligosaccharyl transferase subunit, putative
Leishmania infantum LinJ.35.1160   oligosaccharyl transferase-like protein
Leishmania major LmjF.35.1150   oligosaccharyl transferase-like protein
Leishmania major LmjF.35.1160   oligosaccharyl transferase subunit, putative
Leishmania major LmjF.35.1130   oligosaccharyl transferase-like protein
Leishmania major LmjF.35.1140   oligosaccharyl transferase-like protein
Leishmania mexicana LmxM.34.1160   oligosaccharyl transferase subunit, putative
Leishmania mexicana LmxM.34.1130   oligosaccharyl transferase-like protein
Leishmania mexicana LmxM.34.1140   oligosaccharyl transferase-like protein
Leishmania mexicana LmxM.34.1150   oligosaccharyl transferase-like protein
Loa Loa (eye worm) LOAG_01138   60S ribosomal protein L35
Loa Loa (eye worm) LOAG_11978   hypothetical protein
Loa Loa (eye worm) LOAG_08952   ITM1 protein
Mus musculus ENSMUSG00000032437   STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Mus musculus ENSMUSG00000032116   STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
Neospora caninum NCLIV_031690   Oligosaccharyl transferase-like protein, related
Oryza sativa 4339345   Os05g0519900
Oryza sativa 4337389   Os04g0675500
Plasmodium berghei PBANKA_0931400   dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3, putative
Plasmodium falciparum PF3D7_1116600   dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3, putative
Plasmodium knowlesi PKNH_0914300   dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3, putative
Plasmodium yoelii PY02151   Stt3 protein-related
Saccharomyces cerevisiae YGL022W   dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3
Schistosoma japonicum Sjp_0018570   ko:K00730 dolichyl-diphosphooligosaccharide--protein glycosyltransferase [EC2.4.1.119], putative
Schistosoma japonicum Sjp_0208290   ko:K07151 STT3, subunit of the oligosaccharyltransferase complex, homolog A, putative
Schistosoma mansoni Smp_075860.2   60S ribosomal protein L35
Schistosoma mansoni Smp_158600   oligosaccharyl transferase
Schmidtea mediterranea mk4.000090.04  
Schmidtea mediterranea mk4.013791.00  
Schmidtea mediterranea mk4.024950.00   60S ribosomal protein L35, putative
Schmidtea mediterranea mk4.000090.05  
Trypanosoma brucei gambiense Tbg972.5.6590  
Trypanosoma brucei gambiense Tbg972.5.1270   oligosaccharyl transferase subunit, putative
Trypanosoma brucei Tb927.5.910   oligosaccharyl transferase subunit, putative
Trypanosoma brucei Tb11.v5.0559   oligosaccharyl transferase subunit, putative
Trypanosoma brucei Tb927.5.900   oligosaccharyl transferase subunit, putative
Trypanosoma brucei Tb11.v5.0558   oligosaccharyl transferase subunit, putative
Trypanosoma brucei Tb927.5.890   oligosaccharyl transferase subunit, putative
Trypanosoma congolense TcIL3000_5_530   oligosaccharyl transferase subunit, putative
Trypanosoma cruzi TcCLB.505163.80   oligosaccharyl transferase subunit, putative
Trypanosoma cruzi TcCLB.503467.30   oligosaccharyl transferase subunit, putative
Toxoplasma gondii TGME49_231430   oligosaccharyl transferase stt3 protein, putative
Trichomonas vaginalis TVAG_131010   oligosaccharyl transferase, putative
Trichomonas vaginalis TVAG_230650   60S ribosomal protein L35, putative
Trichomonas vaginalis TVAG_077860   oligosaccharyl transferase, putative
Trichomonas vaginalis TVAG_430200   oligosaccharyl transferase, putative

Essentiality

Tb927.5.900 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.900 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.900 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.900 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.900 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.5.890 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.890 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.890 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.5.890 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.5.910 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.910 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.910 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.910 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T12A2.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_T12A2.2 Caenorhabditis elegans larval arrest wormbase
CELE_T12A2.2 Caenorhabditis elegans sterile wormbase
YGL022W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0931400 Plasmodium berghei Essential plasmo
TGME49_231430 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.5.900 (Trypanosoma brucei), oligosaccharyl transferase subunit, putative
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