Detailed view for Tb927.10.6260

Basic information

TDR Targets ID: 15293
Trypanosoma brucei, ATP-dependent DEAD/H RNA helicase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.3736 | Length (AA): 604 | MW (Da): 68679 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00270   DEAD/DEAH box helicase
PF00271   Helicase conserved C-terminal domain
PF13959   Domain of unknown function (DUF4217)

Gene Ontology

Mouse over links to read term descriptions.
GO:0008026   ATP-dependent helicase activity  
GO:0005524   ATP binding  
GO:0004386   helicase activity  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128169)

Species Accession Gene Product
Arabidopsis thaliana AT5G65900   DEAD-box ATP-dependent RNA helicase 27
Arabidopsis thaliana AT3G18600   DEAD-box ATP-dependent RNA helicase 51
Babesia bovis BBOV_III009130   DEAD/DEAH box domain containing protein
Brugia malayi Bm1_22410   ATP-dependent RNA helicase DDX18
Brugia malayi Bm1_07230   hypothetical protein
Candida albicans CaO19.3962   RNA-dependent helicase (putative).
Candida albicans CaO19.11444   RNA-dependent helicase (putative).
Caenorhabditis elegans CELE_B0511.6   Protein B0511.6
Cryptosporidium hominis Chro.30442   DEAD/DEAH box ATP-dependent RNA helicase
Cryptosporidium parvum cgd3_3920   DEAD/DEAH box ATP-dependent RNA helicase, putative
Dictyostelium discoideum DDB_G0282741   DEAD/DEAH box helicase
Drosophila melanogaster Dmel_CG6375   pitchoune
Echinococcus granulosus EgrG_000127400   DEAD box ATP dependent RNA helicase
Entamoeba histolytica EHI_169630   DEAD/DEAH box helicase, putative
Echinococcus multilocularis EmuJ_000127400   DEAD box ATP dependent RNA helicase
Giardia lamblia GL50803_16887   ATP-dependent RNA helicase HAS1, putative
Homo sapiens ENSG00000088205   DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
Leishmania braziliensis LbrM.35.2030   DEAD box RNA helicase, putative
Leishmania donovani LdBPK_361920.1   DEAD box RNA helicase, putative
Leishmania infantum LinJ.36.1920   DEAD box RNA helicase, putative
Leishmania major LmjF.36.1840   DEAD box RNA helicase, putative
Leishmania mexicana LmxM.36.1840   DEAD box RNA helicase, putative
Loa Loa (eye worm) LOAG_08812   ATP-dependent RNA helicase DDX18
Mus musculus ENSMUSG00000001674   DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
Neospora caninum NCLIV_056340   ATP-dependent RNA helicase DDX55 (EC 3.6.1.-), related
Oryza sativa 4334469   Os03g0802700
Plasmodium berghei PBANKA_1129700   ATP-dependent RNA helicase HAS1, putative
Plasmodium falciparum PF3D7_0630900   ATP-dependent RNA helicase HAS1
Plasmodium knowlesi PKNH_1118600   ATP-dependent RNA helicase HAS1, putative
Plasmodium vivax PVX_114645   ATP-dependent RNA helicase HAS1, putative
Plasmodium yoelii PY00830   probable ATP-dependent RNA helicase has1
Saccharomyces cerevisiae YMR290C   ATP-dependent RNA helicase HAS1
Schistosoma japonicum Sjp_0215350   ko:K01529 DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 [EC:3.6.1.-], putative
Schistosoma mansoni Smp_094020   DEAD box ATP-dependent RNA helicase
Schmidtea mediterranea mk4.008960.00   ATP-dependent RNA helicase DDX18
Schmidtea mediterranea mk4.005410.00   Putative dead box ATP-dependent RNA helicase
Schmidtea mediterranea mk4.015077.00   ATP-dependent RNA helicase DDX18
Schmidtea mediterranea mk4.011697.00   Putative dead box ATP-dependent RNA helicase
Trypanosoma brucei gambiense Tbg972.10.7640   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma brucei Tb11.v5.0279   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma brucei Tb927.10.6260   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma congolense TcIL3000_10_5340   ATP-dependent DEAD/H RNA helicase, putative
Trypanosoma cruzi TcCLB.507641.120   ATP-dependent DEAD/H RNA helicase, putative
Toxoplasma gondii TGME49_312990   DEAD/DEAH box ATP-dependent RNA helicase
Theileria parva TP04_0821   ATP-dependent RNA helicase, putative
Trichomonas vaginalis TVAG_120130   DEAD box ATP-dependent RNA helicase, putative

Essentiality

Tb927.10.6260 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.6260 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.6260 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.6260 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.6260 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0511.6 Caenorhabditis elegans larval arrest wormbase
CELE_B0511.6 Caenorhabditis elegans sterile wormbase
YMR290C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1129700 Plasmodium berghei Essential plasmo
TGME49_312990 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.6260 (Trypanosoma brucei), ATP-dependent DEAD/H RNA helicase, putative
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