Detailed view for PF3D7_1104900

Basic information

TDR Targets ID: 1539
Plasmodium falciparum, calcium/calmodulin-dependent protein kinase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.7534 | Length (AA): 1620 | MW (Da): 195180 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 23 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
163 450 1nxk (A) 51 345 18.00 0 1 0.39 -0.37
166 429 1jks (A) 3 276 20.00 0 0.89 0.39 -0.44
173 352 1om1 (A) 37 200 25.00 0.0000000089 1 0.36 -0.5
188 335 1jks (A) 25 169 30.00 0.000000083 1 0.52 -1.03
473 739 1phk () 17 290 19.00 0 0.75 0.33 0.07
518 737 2eue (A) 99 307 20.00 0 0.4 0.21 -0.03
139 432 4wih (A) 8 297 20.00 0.0000000000055 0.89 0.377581 0.01
151 432 4dfy (A) 19 296 21.00 0.000000000039 1 0.320174 0.22
161 441 3ka0 (A) 49 358 19.00 0.00000017 0.99 0.344557 -0.44
172 307 2w4o (A) 39 172 36.00 0.000066 0.92 0.273951 1.23
178 380 3bkb (A) 563 767 17.00 0.016 0.35 0.203409 0.35
200 430 1fmk (A) 290 522 14.00 0.27 0.47 0.207693 -0.3
200 445 3g5d (A) 290 532 16.00 0.17 1 0.250952 -0.21
232 353 4fg8 (A) 74 195 40.00 0.000063 0.61 0.181309 1.26
633 737 3c0i (A) 180 277 41.00 0.001 0.84 0.392815 0.07
634 724 1vzo (A) 215 302 38.00 0.11 0.99 0.279173 0.27
638 737 4pds (A) 361 467 44.00 0.0000069 0.71 0.225728 0.68
930 1013 3ult (A) 6 89 26.00 0.92 0.03 0.523852 -2.38
161 440 3ka0 (A) 49 358 17.00 0.000000079 0.99 0.362903 -0.45
180 306 2cn8 (A) 233 354 46.00 0.00000097 1 0.291329 1.41
490 903 3n51 (A) 68 489 17.00 0 1 0.32726 0.46
632 736 3c0i (A) 180 277 41.00 0.00098 0.84 0.395414 0.07
1237 1540 4wat (A) 146 492 19.00 0.25 0.02 0.284083 -0.18

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile merozoite, sporozoite, late schizont, Sporozoite. PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, early schizont, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, late trophozoite, Oocyst. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 16 hs, intra-erythrocytic - 32 hs, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 24 hs. Otto TD
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_143222)

Species Accession Gene Product
Babesia bovis BBOV_III007550   protein kinase domain containing protein
Cryptosporidium hominis Chro.20175   protein kinase domain
Cryptosporidium parvum cgd2_1610   protein kinase domain, putative
Neospora caninum NCLIV_016060   protein kinase, putative
Plasmodium berghei PBANKA_0942000   calcium/calmodulin-dependent protein kinase, putative
Plasmodium falciparum PF3D7_1104900   calcium/calmodulin-dependent protein kinase, putative
Plasmodium knowlesi PKNH_0902500   calcium/calmodulin-dependent protein kinase, putative
Plasmodium vivax PVX_090925   protein kinase domain containing protein
Plasmodium yoelii PY02823   Protein kinase domain, putative
Toxoplasma gondii TGME49_239420   protein kinase
Theileria parva TP04_0678   hypothetical protein

Essentiality

PF3D7_1104900 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_239420 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0081 1 0.5
0.0088 0.4477 0.5
0.0056 1 0.5
0.0093 0.8828 0
0.0032 0.5 0.5
0.0063 0.7244 0.7244
0.0059 1 1
0.0022 0.5 0.5
0.0064 0.3377 0
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0033 1 1
0.0007 0.5 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0037 1 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0042 0.5 0.5
0.0018 0.5 0.5
0.0069 0.3067 1
0.0059 1 1
0.0033 0.5 0.5
0.0063 1 1
0.0004 0.5 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0008 0.5 0.5
0.0092 1 0.5
0.0011 1 0.5
0.0029 0.5 0.5
0.0062 0.6935 0
0.0059 1 1
0.0027 1 0.5
0.0067 0.5 0.5
0.0016 0.5 0.5
0.0066 0.3101 0
0.0026 0.5 0.5
0.0036 0.5 0.5
0.0039 0.5 0.5
0.0061 0.6883 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_1104900 (Plasmodium falciparum), calcium/calmodulin-dependent protein kinase, putative
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