Detailed view for Tb927.3.2030

Basic information

TDR Targets ID: 15575
Trypanosoma brucei, acylphosphatase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4627 | Length (AA): 110 | MW (Da): 12370 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00708   Acylphosphatase

Gene Ontology

Mouse over links to read term descriptions.
GO:0003998   acylphosphatase activity  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 110 2lxf (A) 17 121 38.00 0 1 1.45922 -0.28
19 109 3br8 (A) 1 89 39.00 0 1 1.41147 -1.4
19 104 1w2i (A) 3 88 47.00 0 1 1.50822 -1.67

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127552)

Species Accession Gene Product
Arabidopsis thaliana AT5G03370   acylphosphatase family protein
Babesia bovis BBOV_IV011650   acylphosphatase, putative
Cryptosporidium parvum cgd6_4900   acylphosphatase, putative
Drosophila melanogaster Dmel_CG18371   CG18371 gene product from transcript CG18371-RA
Drosophila melanogaster Dmel_CG14022   CG14022 gene product from transcript CG14022-RA
Drosophila melanogaster Dmel_CG34161   CG34161 gene product from transcript CG34161-RA
Drosophila melanogaster Dmel_CG11052   CG11052 gene product from transcript CG11052-RB
Drosophila melanogaster Dmel_CG16870   Acylphosphatase
Drosophila melanogaster Dmel_CG18505   Acylphosphatase 2
Escherichia coli b0968   weak acylphosphatase
Echinococcus granulosus EgrG_000225000   acylphosphatase 1
Echinococcus multilocularis EmuJ_000225000   acylphosphatase 1
Giardia lamblia GL50803_7871   Hypothetical protein
Homo sapiens ENSG00000170634   acylphosphatase 2, muscle type
Homo sapiens ENSG00000119640   acylphosphatase 1, erythrocyte (common) type
Leishmania braziliensis LbrM.25.1520   acylphosphatase, putative
Leishmania infantum LinJ.25.2040   acylphosphatase, putative
Leishmania major LmjF.25.1960   acylphosphatase, putative
Leishmania mexicana LmxM.25.1960   acylphosphatase, putative
Mus musculus 75572   acylphosphatase 2, muscle type
Mus musculus ensembl-mmu:ENSMUSG00000008822   acylphosphatase 1, erythrocyte (common) type
Mycobacterium tuberculosis Rv2922A   Probable acylphosphatase AcyP (acylphosphate phosphohydrolase)
Oryza sativa 4340703   Os06g0264700
Oryza sativa 4340702   Os06g0264600
Plasmodium berghei PBANKA_0936100   acylphosphatase, putative
Plasmodium falciparum PF3D7_1111500   acylphosphatase, putative
Plasmodium knowlesi PKNH_0909200   acylphosphatase, putative
Plasmodium vivax PVX_091225   acylphosphatase, putative
Schistosoma japonicum Sjp_0098030   ko:K01512 acylphosphatase [EC3.6.1.7], putative
Schmidtea mediterranea mk4.017018.00  
Trypanosoma brucei gambiense Tbg972.3.1960   acylphosphatase, putative
Trypanosoma brucei Tb927.3.2030   acylphosphatase, putative
Trypanosoma congolense TcIL3000_3_1120   acylphosphatase, putative
Trypanosoma cruzi TcCLB.504047.34   acylphosphatase, putative
Toxoplasma gondii TGME49_258940   acylphosphatase family protein
Theileria parva TP01_1141   acylphosphatase, putative

Essentiality

Tb927.3.2030 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.2030 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.2030 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.2030 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.2030 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0968 Escherichia coli non-essential goodall
TGME49_258940 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.3.2030 (Trypanosoma brucei), acylphosphatase, putative
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