Detailed view for PF3D7_1110500

Basic information

TDR Targets ID: 1586
Plasmodium falciparum, vacuolar protein sorting-associated protein 35, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.3442 | Length (AA): 1050 | MW (Da): 123468 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03635   Vacuolar protein sorting-associated protein 35

Gene Ontology

Mouse over links to read term descriptions.
GO:0042147   retrograde transport, endosome to Golgi  
GO:0030906   retromer complex, inner shell  
GO:0003674   molecular_function  
GO:0030117   membrane coat  
GO:0015031   protein transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
645 1031 2r17 (C) 483 780 23.00 0 1 0.274171 0.19
808 1030 2r17 (D) 558 772 31.00 0 1 0.482181 -0.31
387 484 3ult (A) 12 118 26.00 0.31 0.05 0.223167 -0.97
645 1034 2r17 (C) 483 780 23.00 0 1 0.31837 -0.34
811 1033 2r17 (D) 558 772 31.00 0 1 0.480876 -0.31

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile gametocyte, early schizont, late schizont, late trophozoite, Ring, Sporozoite. PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile early trophozoite. PlasmoDB
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127886)

Species Accession Gene Product
Arabidopsis thaliana AT3G51310   vacuolar protein sorting-associated protein 35C
Arabidopsis thaliana AT1G75850   vacuolar protein sorting-associated protein 35B
Arabidopsis thaliana AT2G17790   vacuolar protein sorting-associated protein 35A
Babesia bovis BBOV_IV009740   vacuolar protein sorting-associated protein 35, putative
Brugia malayi Bm1_44045   Vacuolar protein sorting 35
Candida albicans CaO19.14164   retrieval of vacuolar sorting receptor
Candida albicans CaO19.6875   retrieval of vacuolar sorting receptor
Caenorhabditis elegans CELE_F59G1.3   Protein VPS-35
Cryptosporidium hominis Chro.40270   vacuolar sorting protein 35
Cryptosporidium parvum cgd4_2390   conserved hypothetical protein
Dictyostelium discoideum DDB_G0293218   vacuolar sorting protein 35
Drosophila melanogaster Dmel_CG5625   Vacuolar protein sorting 35
Echinococcus granulosus EgrG_000083200   vacuolar protein sorting 35
Entamoeba histolytica EHI_086580   vacuolar protein sorting-associated protein 35 , putative
Entamoeba histolytica EHI_041950   vacuolar protein sorting-associated protein 35 , putative
Entamoeba histolytica EHI_002990   vacuolar protein sorting-associated protein 35 , putative
Echinococcus multilocularis EmuJ_000083200   vacuolar protein sorting 35
Homo sapiens 55737   vacuolar protein sorting 35 homolog (S. cerevisiae)
Leishmania braziliensis LbrM.21.0530   vacuolar sorting-associated-like protein
Leishmania donovani LdBPK_210530.1   vacuolar sorting-associated-like protein
Leishmania infantum LinJ.21.0530   vacuolar sorting-associated-like protein
Leishmania major LmjF.21.0470   vacuolar sorting-associated-like protein
Leishmania mexicana LmxM.21.0470   vacuolar sorting-associated-like protein
Loa Loa (eye worm) LOAG_04650   vacuolar protein sorting 35
Mus musculus ENSMUSG00000031696   vacuolar protein sorting 35
Neospora caninum NCLIV_017680   hypothetical protein
Oryza sativa 4334459   Os03g0801600
Plasmodium berghei PBANKA_0937100   vacuolar protein sorting-associated protein 35, putative
Plasmodium falciparum PF3D7_1110500   vacuolar protein sorting-associated protein 35, putative
Plasmodium knowlesi PKNH_0908200   vacuolar protein sorting-associated protein 35, putative
Plasmodium vivax PVX_091175   vacuolar protein sorting-associated protein 35, putative
Plasmodium yoelii PY01746   vacuolar protein sorting 35-related
Saccharomyces cerevisiae YJL154C   Vps35p
Schistosoma japonicum Sjp_0025740   Vacuolar protein sorting-associated protein 35, putative
Schistosoma mansoni Smp_173330   vacuolar sorting protein
Schmidtea mediterranea mk4.001986.07   Vacuolar protein sorting-associated protein 35
Schmidtea mediterranea mk4.001986.06   Vacuolar protein sorting-associated protein 35
Schmidtea mediterranea mk4.002290.02   Vacuolar protein sorting-associated protein 35
Schmidtea mediterranea mk4.001986.08   Vacuolar protein sorting-associated protein 35
Trypanosoma brucei gambiense Tbg972.10.2750   vacuolar protein sorting-associated protein 35, putative
Trypanosoma brucei Tb927.10.2270   vacuolar protein sorting-associated protein 35, putative
Trypanosoma congolense TcIL3000_10_1880   vacuolar protein sorting-associated protein 35, putative
Trypanosoma cruzi TcCLB.511367.170   vacuolar protein sorting-associated protein 35, putative
Toxoplasma gondii TGME49_242660   vacuolar sorting protein 35, putative
Theileria parva TP01_0794   vacuolar sorting protein 35, putative
Trichomonas vaginalis TVAG_198400   vacuolar sorting protein, putative
Trichomonas vaginalis TVAG_216890   vacuolar sorting protein, putative
Trichomonas vaginalis TVAG_468000   vacuolar sorting protein, putative

Essentiality

PF3D7_1110500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.2270 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.2270 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.2270 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.2270 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F59G1.3 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_0937100 Plasmodium berghei Essential plasmo
TGME49_242660 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_1110500 (Plasmodium falciparum), vacuolar protein sorting-associated protein 35, putative
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