Detailed view for Tb927.3.3590

Basic information

TDR Targets ID: 16092
Trypanosoma brucei, U3 small nucleolar ribonucleoprotein protein MPP10, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.5871 | Length (AA): 672 | MW (Da): 75272 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04006   Mpp10 protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0034457   GO:Mpp10 complex  

GO:0005732   small nucleolar ribonucleoprotein complex  
GO:0005634   nucleus  
GO:0006364   rRNA processing  

Metabolic Pathways

Ribosome biogenesis in eukaryotes (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
230 412 5h78 (A) 7 189 11.00 0 0.01 0.456521 -0.97
502 669 1c1g (A) 2 167 28.00 0.15 0.03 0.2204 1.12

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127902)

Species Accession Gene Product
Arabidopsis thaliana AT5G66540   hypothetical protein
Babesia bovis BBOV_IV001330   U3 small nucleolar ribonucleoprotein protein, putative
Brugia malayi Bm1_21335   Mpp10 protein
Candida albicans CaO19.9471   similar to U3 snoRNP binding protein, part of the pre-18S rRNA processome
Candida albicans CaO19.1915   U3 small nucleolar ribonucleoprotein (snoRNP)
Caenorhabditis elegans CELE_Y75B8A.7   Protein Y75B8A.7
Cryptosporidium hominis Chro.70400   At5g66540/K1F13_21
Cryptosporidium parvum cgd7_3590   Mpp10 family of U3 processosome protein
Dictyostelium discoideum DDB_G0279245   U3 small nucleolar ribonucleoprotein
Drosophila melanogaster Dmel_CG13097   CG13097 gene product from transcript CG13097-RA
Echinococcus granulosus EgrG_000914700   U3 small nucleolar ribonucleoprotein
Entamoeba histolytica EHI_142200   U3 small nucleolar ribonucleoprotein subunit, putative
Entamoeba histolytica EHI_048860   U3 small nucleolar ribonucleo protein MPP10, putative
Echinococcus multilocularis EmuJ_000914700   U3 small nucleolar ribonucleoprotein
Giardia lamblia GL50803_9274   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Homo sapiens ENSG00000124383   M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein)
Leishmania braziliensis LbrM.29.0770   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Leishmania donovani LdBPK_290780.1   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Leishmania infantum LinJ.29.0780   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Leishmania major LmjF.29.0750   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Leishmania mexicana LmxM.08_29.0750   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Loa Loa (eye worm) LOAG_08098   Mpp10 protein
Mus musculus ENSMUSG00000030521   M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein)
Neospora caninum NCLIV_055310   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Oryza sativa 4351433   Os12g0133900
Oryza sativa 4349717   Os11g0137100
Onchocerca volvulus OVOC9642   U3 small nucleolar ribonucleoprotein protein MPP10 homolog
Plasmodium berghei PBANKA_0511200   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Plasmodium falciparum PF3D7_1027100   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Plasmodium knowlesi PKNH_0611500   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Plasmodium vivax PVX_111365   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Plasmodium yoelii PY06994   Arabidopsis thaliana At5g66540/K1F13_21
Saccharomyces cerevisiae YJR002W   Mpp10p
Schistosoma japonicum Sjp_0045690   IPR007151,Mpp10 protein,domain-containing
Schistosoma mansoni Smp_023660   U3 small nucleolar ribonucleoprotein protein MPP10
Trypanosoma brucei gambiense Tbg972.3.3860   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Trypanosoma brucei Tb927.3.3590   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Trypanosoma congolense TcIL3000_3_2280   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Trypanosoma cruzi TcCLB.503811.20   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Trypanosoma cruzi TcCLB.506989.180   U3 small nucleolar ribonucleoprotein protein MPP10, putative
Toxoplasma gondii TGME49_311410   M phase phosphoprotein MPP10, putative
Theileria parva TP01_0160   hypothetical protein
Trichomonas vaginalis TVAG_166990   U3 small nucleolar ribonucleoprotein mpp10, putative

Essentiality

Tb927.3.3590 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.3590 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.3.3590 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.3590 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.3590 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y75B8A.7 Caenorhabditis elegans larval arrest wormbase
CELE_Y75B8A.7 Caenorhabditis elegans larval lethal wormbase
CELE_Y75B8A.7 Caenorhabditis elegans slow growth wormbase
CELE_Y75B8A.7 Caenorhabditis elegans sterile wormbase
YJR002W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0511200 Plasmodium berghei Essential plasmo
TGME49_311410 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.3.3590 (Trypanosoma brucei), U3 small nucleolar ribonucleoprotein protein MPP10, putative
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