Detailed view for Tb927.10.2940

Basic information

TDR Targets ID: 16449
Trypanosoma brucei, Soluble NSF attachment protein, SNAP, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.3901 | Length (AA): 354 | MW (Da): 38469 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF14938   Soluble NSF attachment protein, SNAP

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  
GO:0005488   binding  
GO:0006886   intracellular protein transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130191)

Species Accession Gene Product
Arabidopsis thaliana AT4G20410   gamma-soluble NSF attachment protein
Brugia malayi Bm1_52430   NSF attachment protein
Caenorhabditis elegans CELE_Y59A8B.8   Protein Y59A8B.8
Caenorhabditis elegans CELE_Y59A8B.25   Protein Y59A8B.25
Dictyostelium discoideum DDB_G0269186   soluble NSF attachment protein gamma isoform
Drosophila melanogaster Dmel_CG6208   gamma Soluble NSF attachment protein 2
Drosophila melanogaster Dmel_CG3988   gamma Soluble NSF attachment protein 1
Echinococcus granulosus EgrG_000924400   Gamma soluble NSF attachment protein
Entamoeba histolytica EHI_136210   soluble NSF attachment protein gamma isoform, putative
Echinococcus multilocularis EmuJ_000924400   Gamma soluble NSF attachment protein
Homo sapiens ENSG00000134265   N-ethylmaleimide-sensitive factor attachment protein, gamma
Leishmania braziliensis LbrM.13.1300   hypothetical protein, conserved
Leishmania donovani LdBPK_340560.1   Gamma-soluble NSF attachment protein (SNAP-gamma) (N-ethylmaleimide-sensitive factor attachment protein, gamma), putative
Leishmania infantum LinJ.34.0560   hypothetical protein, conserved
Leishmania major LmjF.34.0540   Gamma-soluble NSF attachment protein (SNAP-gamma) (N-ethylmaleimide-sensitive factor attachment protein, gamma), putative
Leishmania mexicana LmxM.33.0540   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_03102   NSF attachment protein
Mus musculus ENSMUSG00000024581   N-ethylmaleimide sensitive fusion protein attachment protein gamma
Neospora caninum NCLIV_009590   GI20748, related
Oryza sativa 4337014   Os04g0618500
Schistosoma japonicum Sjp_0025720   Gamma-soluble NSF attachment protein, putative
Schistosoma mansoni Smp_052740   gamma-soluble nsf attachment protein (snap)
Schmidtea mediterranea mk4.000005.07   Gamma-soluble NSF attachment protein
Trypanosoma brucei gambiense Tbg972.10.3680   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.2940   Soluble NSF attachment protein, SNAP, putative
Trypanosoma congolense TcIL3000_0_22500   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.503697.110   Soluble NSF attachment protein, SNAP, putative
Trypanosoma cruzi TcCLB.504105.190   Soluble NSF attachment protein, SNAP, putative
Toxoplasma gondii TGME49_320690   gamma-soluble NSF attachment protein, putative
Trichomonas vaginalis TVAG_432640   gamma-soluble NSF attachment protein, putative

Essentiality

Tb927.10.2940 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.2940 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.2940 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.2940 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.2940 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_320690 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.2940 (Trypanosoma brucei), Soluble NSF attachment protein, SNAP, putative
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