Detailed view for Tb927.10.5310

Basic information

TDR Targets ID: 16485
Trypanosoma brucei, SNF1-related protein kinases, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.4372 | Length (AA): 729 | MW (Da): 80620 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 469 4rer (A) 12 544 33.00 0 1 0.812675 0.78
2 372 4rer (A) 12 366 43.00 0 1 0.917716 0.3
5 273 3mn3 (A) 52 318 52.00 0 1 0.965799 -0.78
8 261 2bdw (A) 13 271 42.00 0 1 0.926422 -1.07
621 704 3t4n (A) 506 626 24.00 0 0.61 0.208926 -0.21

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126655)

Species Accession Gene Product
Arabidopsis thaliana AT5G39440   SNF1-related protein kinase 1.3
Arabidopsis thaliana AT3G01090   SNF1-related protein kinase catalytic subunit alpha KIN10
Arabidopsis thaliana AT3G29160   SNF1-related protein kinase catalytic subunit alpha KIN11
Babesia bovis BBOV_III009120   protein kinase domain containing protein
Brugia malayi Bm1_37010   putative serine/threonine kinase SADA gamma
Brugia malayi Bm1_37015   hypothetical protein
Brugia malayi Bm1_50100   EST embl|AI107989|AI107989 comes from the 3' UTR, putative
Candida albicans CaO19.9491   protein serine/threonine kinase
Candida albicans CaO19.4308   similar to S. cerevisiae HSL1 (YKL101W) serine/threonine-protein kinase involved in cell cycle control
Candida albicans CaO19.8280   bud neck kinase
Candida albicans CaO19.11784   similar to S. cerevisiae HSL1 (YKL101W) serine/threonine-protein kinase involved in cell cycle control
Candida albicans CaO19.1936   protein serine/threonine kinase
Candida albicans CaO19.663   bud neck kinase
Caenorhabditis elegans CELE_F15A2.6   Protein SAD-1, isoform A
Caenorhabditis elegans CELE_T01C8.1   Protein AAK-2, isoform C
Cryptosporidium parvum cgd6_650   OSK4, putative
Dictyostelium discoideum DDB_G0277905   AMP-activated protein kinase alpha subunit
Dictyostelium discoideum DDB_G0280133   CAMKL family protein kinase
Dictyostelium discoideum DDB_G0277165   BRSK subfamily protein kinase
Drosophila melanogaster Dmel_CG3051   AMP-activated protein kinase alpha subunit
Drosophila melanogaster Dmel_CG6114   sugar-free frosting
Echinococcus granulosus EgrG_000708800   5' AMP activated protein kinase catalytic
Echinococcus granulosus EgrG_000915400   serine:threonine kinase SAD 1
Entamoeba histolytica EHI_110010   serine/threonine protein kinase, putative
Echinococcus multilocularis EmuJ_000708800   5' AMP activated protein kinase catalytic
Echinococcus multilocularis EmuJ_000915400   serine:threonine kinase SAD 1
Giardia lamblia GL50803_14364   Kinase, CAMK CAMKL
Giardia lamblia GL50803_16034   Kinase, CAMK CAMKL
Homo sapiens ENSG00000160469   BR serine/threonine kinase 1
Homo sapiens ENSG00000162409   protein kinase, AMP-activated, alpha 2 catalytic subunit
Homo sapiens ENSG00000174672   BR serine/threonine kinase 2
Homo sapiens ENSG00000132356   protein kinase, AMP-activated, alpha 1 catalytic subunit
Leishmania braziliensis LbrM.29.1990   serine/threonine protein kinase, putative,protein kinase, putative
Leishmania braziliensis LbrM.35.1020   protein kinase, putative,SNF1-related protein kinases, putative
Leishmania donovani LdBPK_360960.1   SNF1-related protein kinases, putative
Leishmania donovani LdBPK_292140.1   5'-AMP-activated protein kinase catalytic subunit alpha, putative
Leishmania infantum LinJ.36.0960   protein kinase, putative,SNF1-related protein kinases, putative
Leishmania infantum LinJ.29.2140   serine/threonine protein kinase, putative,protein kinase, putative
Leishmania major LmjF.36.0900   protein kinase, putative,SNF1-related protein kinases, putative
Leishmania major LmjF.29.2020   serine/threonine protein kinase, putative,protein kinase, putative
Leishmania mexicana LmxM.08_29.2020   serine/threonine protein kinase, putative,protein kinase, putative
Leishmania mexicana LmxM.36.0900   protein kinase, putative
Loa Loa (eye worm) LOAG_01209   hypothetical protein
Loa Loa (eye worm) LOAG_01208   hypothetical protein
Loa Loa (eye worm) LOAG_05061   CAMK/CAMKL/AMPK protein kinase
Loa Loa (eye worm) LOAG_01207   CAMK/CAMKL/BRSK protein kinase
Loa Loa (eye worm) LOAG_01206   hypothetical protein
Mus musculus ENSMUSG00000035390   BR serine/threonine kinase 1
Mus musculus ENSMUSG00000053046   BR serine/threonine kinase 2
Mus musculus ENSMUSG00000028518   protein kinase, AMP-activated, alpha 2 catalytic subunit
Mus musculus ENSMUSG00000050697   protein kinase, AMP-activated, alpha 1 catalytic subunit
Neospora caninum NCLIV_033490   hypothetical protein
Oryza sativa 4332495   Os03g0289100
Oryza sativa 4339410   Os05g0530500
Oryza sativa 4345873   Os08g0484600
Plasmodium falciparum PF3D7_1454300   serine/threonine protein kinase KIN
Plasmodium knowlesi PKNH_1227800   serine/threonine protein kinase KIN, putative
Plasmodium vivax PVX_117765   serine/threonine protein kinase KIN, putative
Saccharomyces cerevisiae YDR507C   Gin4p
Saccharomyces cerevisiae YCL024W   Kcc4p
Saccharomyces cerevisiae YKL101W   Hsl1p
Saccharomyces cerevisiae YDR477W   AMP-activated serine/threonine-protein kinase catalytic subunit SNF1
Schistosoma japonicum Sjp_0202490   ko:K07198 5'-AMP-activated protein kinase, catalytic alpha subunit, putative
Schistosoma japonicum Sjp_0308150   HD domain-containing protein 2 homolog, putative
Schistosoma japonicum Sjp_0007470   ko:K08796 BR serine/threonine kinase, putative
Schistosoma japonicum Sjp_0007320   MAP/microtubule affinity-regulating kinase 4, putative
Schistosoma mansoni Smp_134910.1   serine/threonine protein kinase
Schistosoma mansoni Smp_169900.1   serine/threonine protein kinase
Schistosoma mansoni Smp_169900.2   serine/threonine protein kinase
Schistosoma mansoni Smp_142990   serine/threonine protein kinase
Schistosoma mansoni Smp_134910.2   serine/threonine protein kinase
Schmidtea mediterranea mk4.018157.00  
Schmidtea mediterranea mk4.016978.00  
Schmidtea mediterranea mk4.025704.00  
Schmidtea mediterranea mk4.030606.00   AMP-activated protein kinase alpha subunit
Schmidtea mediterranea mk4.001868.03  
Schmidtea mediterranea mk4.005918.01   AMP-activated protein kinase alpha subunit
Schmidtea mediterranea mk4.001025.08   Serine/threonine-protein kinase BRSK2
Trypanosoma brucei gambiense Tbg972.10.6470   protein kinase, putative,SNF1-related protein kinases, putative
Trypanosoma brucei gambiense Tbg972.3.5030   serine/threonine protein kinase, putative,protein kinase, putative
Trypanosoma brucei Tb927.10.5310   SNF1-related protein kinases, putative
Trypanosoma brucei Tb927.3.4560   5'-AMP-activated protein kinase catalytic subunit alpha, putative
Trypanosoma congolense TcIL3000_10_4460   SNF1-related protein kinases, putative
Trypanosoma cruzi TcCLB.506679.80   SNF1-related protein kinases, putative
Trypanosoma cruzi TcCLB.510329.210   5'-AMP-activated protein kinase catalytic subunit alpha, putative
Toxoplasma gondii TGME49_233905   histone kinase SNF1, putative
Theileria parva TP04_0818   5'-AMP-activated protein kinase, putative
Trichomonas vaginalis TVAG_309990   CAMK family protein kinase
Trichomonas vaginalis TVAG_324230   CAMK family protein kinase
Trichomonas vaginalis TVAG_250560   CAMK family protein kinase
Trichomonas vaginalis TVAG_094470   CAMK family protein kinase
Trichomonas vaginalis TVAG_055350   CAMK family protein kinase
Trichomonas vaginalis TVAG_232610   CAMK family protein kinase
Trichomonas vaginalis TVAG_402790   CAMK family protein kinase
Trichomonas vaginalis TVAG_350500   CAMK family protein kinase
Trichomonas vaginalis TVAG_480240   CAMK family protein kinase
Trichomonas vaginalis TVAG_367400   CAMK family protein kinase
Trichomonas vaginalis TVAG_365840   CAMK family protein kinase
Trichomonas vaginalis TVAG_320990   CAMK family protein kinase
Trichomonas vaginalis TVAG_053820   CAMK family protein kinase
Trichomonas vaginalis TVAG_456650   CAMK family protein kinase
Trichomonas vaginalis TVAG_005980   CAMK family protein kinase
Trichomonas vaginalis TVAG_065500   CAMK family protein kinase
Trichomonas vaginalis TVAG_021680   CAMK family protein kinase

Essentiality

Tb927.10.5310 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.4560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.4560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.4560 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.3.4560 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.5310 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.5310 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.5310 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.5310 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
YKL101W Saccharomyces cerevisiae inviable yeastgenome
TGME49_233905 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.9

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens protein kinase, AMP-activated, alpha 1 catalytic subunit Compounds References
Homo sapiens BR serine/threonine kinase 1 Compounds References
Homo sapiens protein kinase, AMP-activated, alpha 2 catalytic subunit Compounds References
Rattus norvegicus AMP-activated protein kinase, alpha-2 subunit Compounds References
Homo sapiens BR serine/threonine kinase 2 Compounds References
Rattus norvegicus AMP-activated protein kinase, alpha-1 subunit Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus MAP kinase p38 alpha 360 aa 26.5% 291 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 30.7% 290 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 26.1% 203 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 33.8% 157 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 28.9% 291 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 21.5% 307 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 30.3% 261 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 27.5% 338 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 31.6% 294 aa Compounds References
Sus scrofa Casein kinase I isoform alpha 125 aa 26.8% 112 aa Compounds References
Patiria pectinifera Cdc2 300 aa 28.7% 289 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0036 0.5 0.5
0.0012 0.5 0.5
0.0029 0.5 0.5
0.0056 1 0.5
0.0081 0.5 0.5
0.0022 0.5 0.5
0.0011 1 0.5
0.0064 0.3377 0
0.0027 1 0.5
0.0093 0.8828 0
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0069 0.3067 1
0.0008 0.5 0.5
0.0091 1 0.5
0.0059 1 1
0.0012 0.5 0.5
0.0004 0.5 0.5
0.0023 0.5 0.5
0.0039 0.5 0.5
0.0016 0.5 0.5
0.0003 0.5 0.5
0.0066 0.3101 0
0.0007 0.5 0.5
0.0033 1 0.5
0.0018 0.5 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.2614
0.0016 0.5 0.5
0.0042 0.5 0.5
0.0063 1 0.5
0.0039 0.9485 0.5
0.0037 1 0.5
0.0067 0.5 0.5
0.0062 0.6935 0
0.0081 1 0.5
0.0007 0.5 0.5
0.0059 1 1
0.0007 0.5 0.5
0.0059 1 1
0.0088 0.4477 0.5
0.0063 0.7244 0.2543
0.0092 1 0.5
0.0032 0.5 0.5
0.0033 0.5 0.5
0.0026 0.5 0.5
0.0061 0.6883 0.5304

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.10.5310 (Trypanosoma brucei), SNF1-related protein kinases, putative
Title for this comment
Comment