Detailed view for Tb927.6.1580

Basic information

TDR Targets ID: 16613
Trypanosoma brucei, polynucleotide kinase 3'-phosphatase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.6091 | Length (AA): 476 | MW (Da): 53126 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08645   Polynucleotide kinase 3 phosphatase
PF13671   AAA domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128311)

Species Accession Gene Product
Arabidopsis thaliana AT3G14890   polynucleotide 3'-phosphatase ZDP
Babesia bovis BBOV_IV000690   polynucleotide kinase 3'-phosphatase, putative
Brugia malayi Bm1_40730   DNA 3'-phosphatase family protein
Candida albicans CaO19.9121   similar to S. pombe pnk1 (SPAC23C11.04c ) 5'kinase/DNA 3' phosphatase involved in DNA repair
Candida albicans CaO19.1547   similar to S. pombe pnk1 (SPAC23C11.04c ) 5'kinase/DNA 3' phosphatase involved in DNA repair
Caenorhabditis elegans CELE_F21D5.5   Protein F21D5.5
Dictyostelium discoideum DDB_G0281229   SAP DNA-binding domain-containing protein
Drosophila melanogaster Dmel_CG9601   CG9601 gene product from transcript CG9601-RA
Echinococcus granulosus EgrG_000166600   bifunctional polynucleotide
Entamoeba histolytica EHI_148430   polynucleotide kinase-3-phosphatase, putative
Echinococcus multilocularis EmuJ_000166600   bifunctional polynucleotide
Homo sapiens ENSG00000039650   polynucleotide kinase 3'-phosphatase
Leishmania donovani LdBPK_300180.1   hypothetical protein
Loa Loa (eye worm) LOAG_09075   hypothetical protein
Mus musculus ENSMUSG00000002963   polynucleotide kinase 3'- phosphatase
Neospora caninum NCLIV_003010   hypothetical protein
Oryza sativa 4327876   Os01g0737300
Onchocerca volvulus OVOC5322   Bifunctional polynucleotide phosphatase\/kinase homolog
Plasmodium berghei PBANKA_1139200   bifunctional polynucleotide phosphatase/kinase, putative
Plasmodium falciparum PF3D7_1363200   bifunctional polynucleotide phosphatase/kinase
Plasmodium knowlesi PKNH_1108100   bifunctional polynucleotide phosphatase/kinase, putative
Plasmodium vivax PVX_115115   bifunctional polynucleotide phosphatase/kinase, putative
Plasmodium yoelii PY05803   hypothetical protein
Saccharomyces cerevisiae YMR156C   Tpp1p
Schistosoma japonicum Sjp_0069760   ko:K08073 polynucleotide 5'-hydroxyl-kinase [EC2.7.1.78], putative
Schistosoma mansoni Smp_169740   polynucleotide kinase-3'-phosphatase
Schmidtea mediterranea mk4.000725.07   Bifunctional polynucleotide phosphatase/kinase
Trypanosoma brucei gambiense Tbg972.6.1250   polynucleotide kinase 3-phosphatase, putative
Trypanosoma brucei Tb927.6.1580   polynucleotide kinase 3'-phosphatase, putative
Trypanosoma congolense TcIL3000_6_1080   polynucleotide kinase 3'-phosphatase, putative
Trypanosoma congolense TcIL3000_0_16570   polynucleotide kinase 3'-phosphatase, putative
Trypanosoma cruzi TcCLB.505807.190   polynucleotide kinase 3'-phosphatase, putative
Trypanosoma cruzi TcCLB.507017.50   polynucleotide kinase 3'-phosphatase, putative
Theileria parva TP01_0091   hypothetical protein

Essentiality

Tb927.6.1580 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1580 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.1580 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.1580 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1580 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F21D5.5 Caenorhabditis elegans larval arrest wormbase
CELE_F21D5.5 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.6.1580 (Trypanosoma brucei), polynucleotide kinase 3'-phosphatase, putative
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