Detailed view for Tb927.8.550

Basic information

TDR Targets ID: 16654
Trypanosoma brucei, peptide methionine sulfoxide reductase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.1415 | Length (AA): 177 | MW (Da): 20367 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01625   Peptide methionine sulfoxide reductase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008113   peptide-methionine-(S)-S-oxide reductase activity  
GO:0005575   cellular_component  
GO:0055114   oxidation reduction  
GO:0019538   protein metabolic process  
GO:0006464   protein modification process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 173 1nwa (A) 1 155 43.00 0 1 1.4416 -0.6
4 177 4gwb (A) 2 159 39.00 0 1 1.38275 -0.44
4 172 1ff3 (A) 42 199 39.00 0 1 1.4285 -0.68
6 176 3pim (A) 18 180 46.00 0 1 1.423 -0.59
6 171 2j89 (A) 39 189 43.00 0 1 1.40375 -0.68

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126847)

Species Accession Gene Product
Arabidopsis thaliana AT5G61640   peptide methionine sulfoxide reductase A1
Arabidopsis thaliana AT4G25130   peptide methionine sulfoxide reductase A4
Arabidopsis thaliana AT5G07460   peptide methionine sulfoxide reductase A2
Arabidopsis thaliana AT2G18030   peptide methionine sulfoxide reductase A5
Arabidopsis thaliana AT5G07470   peptide methionine sulfoxide reductase A3
Brugia malayi Bm1_10795   Peptide methionine sulfoxide reductase family protein
Candida albicans CaO19.2028   Peptide Methionine Sulfoxide Reductase 1
Candida albicans CaO19.9576   Peptide Methionine Sulfoxide Reductase 1
Caenorhabditis elegans CELE_F43E2.5   Protein MSRA-1
Dictyostelium discoideum DDB_G0276579   hypothetical protein
Drosophila melanogaster Dmel_CG7266   Ecdysone-induced protein 28/29kD
Escherichia coli b4219   methionine sulfoxide reductase A
Echinococcus granulosus EgrG_000926700   peptide methionine sulfoxide reductase
Echinococcus multilocularis EmuJ_000926700   peptide methionine sulfoxide reductase
Giardia lamblia GL50803_4946   Peptide methionine sulfoxide reductase msrA
Homo sapiens ENSG00000175806   methionine sulfoxide reductase A
Leishmania braziliensis LbrM.07.1220   peptide methionine sulfoxide reductase-like,peptide Met(O) reductase, putative,methionine-S-sulfoxide reductase, putative
Leishmania donovani LdBPK_071320.1   peptide methionine sulfoxide reductase-like
Leishmania infantum LinJ.07.1320   peptide methionine sulfoxide reductase-like,peptide Met(O) reductase, putative,methionine-S-sulfoxide reductase, putative
Leishmania major LmjF.07.1140   peptide methionine sulfoxide reductase-like,peptide Met(O) reductase, putative,methionine-S-sulfoxide reductase, putative
Leishmania mexicana LmxM.07.1140   peptide methionine sulfoxide reductase-like,peptide Met(O) reductase, putative,methionine-S-sulfoxide reductase, putative
Loa Loa (eye worm) LOAG_02161   peptide methionine sulfoxide reductase
Mycobacterium leprae ML2647   PROBABLE PEPTIDE METHIONINE SULFOXIDE REDUCTASE MSRA (PROTEIN-METHIONINE-S-OXIDE REDUCTASE) (PEPTIDE MET(O) REDUCTASE)
Mus musculus ENSMUSG00000054733   methionine sulfoxide reductase A
Mycobacterium tuberculosis Rv0137c   Probable peptide methionine sulfoxide reductase MsrA (protein-methionine-S-oxide reductase) (peptide met(O) reductase)
Mycobacterium ulcerans MUL_4773   methionine sulfoxide reductase A
Neospora caninum NCLIV_047240   YALI0E30151p, related
Oryza sativa 4340052   Os06g0138100
Oryza sativa 4349402   Os10g0563600
Oryza sativa 4336192   Os04g0482000
Saccharomyces cerevisiae YER042W   Mxr1p
Schmidtea mediterranea mk4.005463.04   Mitochondrial peptide methionine sulfoxide reductase
Schmidtea mediterranea mk4.007364.01   Mitochondrial peptide methionine sulfoxide reductase
Trypanosoma brucei gambiense Tbg972.8.120   peptide methionine sulfoxide reductase, putative,peptide Met(O) reductase, putative,methionine-S-sulfoxide reductase, putative
Trypanosoma brucei Tb927.8.550   peptide methionine sulfoxide reductase, putative
Trypanosoma congolense TcIL3000_8_20   peptide methionine sulfoxide reductase, putative
Trypanosoma congolense TcIL3000_0_23940   peptide methionine sulfoxide reductase, putative
Trypanosoma cruzi TcCLB.509611.180   peptide methionine sulfoxide reductase, putative
Trypanosoma cruzi TcCLB.510855.10   peptide methionine sulfoxide reductase, putative
Toxoplasma gondii TGME49_225470   peptide methionine sulfoxide reductase
Treponema pallidum TP0633   bifunctional methionine sulfoxide reductase B/A protein
Trichomonas vaginalis TVAG_018970   methionine sulfoxide reductase, putative
Trichomonas vaginalis TVAG_034690   methionine sulfoxide reductase, putative

Essentiality

Tb927.8.550 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu138 Mycobacterium tuberculosis non-essential nmpdr
Tb927.8.550 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.550 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.8.550 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.550 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b4219 Escherichia coli non-essential goodall
TGME49_225470 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Bos taurus Mitochondrial peptide methionine sulfoxide reductase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0053 0.2686 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.8.550 (Trypanosoma brucei), peptide methionine sulfoxide reductase, putative
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