Detailed view for PF3D7_1119100

Basic information

TDR Targets ID: 1667
Plasmodium falciparum, tRNA m(1)G methyltransferase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.9462 | Length (AA): 324 | MW (Da): 38793 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01746   tRNA (Guanine-1)-methyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0008168   methyltransferase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
100 285 4jwj (A) 82 274 27.00 0.00000000013 1 0.978874 -0.9
110 286 4fmw (A) 98 275 31.00 0 1 1.0561 -1.28
114 286 4jwh (A) 96 272 34.00 0 1 1.03805 -1.1
8 137 4tko (B) 57 227 36.00 0.16 0.24 0.372935 0.76
23 67 2j1d (G) 981 1025 22.00 0.38 0 0.673289 -3.24
100 285 4jwj (A) 82 274 27.00 0.0000000005 1 0.920274 -0.27
110 286 4fmw (A) 98 275 31.00 0 1 1.0767 -1.5

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 24 hs, gametocyte, sporozoite, early ring, early trophozoite, late ring, late trophozoite, Oocyst, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, early schizont, Ring, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, late schizont. Otto TD PlasmoDB
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127454)

Species Accession Gene Product
Arabidopsis thaliana AT5G47680   protein tRNA MODIFICATION 10
Babesia bovis BBOV_II000470   predicted protein
Babesia bovis BBOV_III002590   tRNA (guanine-N1)-methyltransferase family protein
Brugia malayi Bm1_22080   hypothetical protein
Candida albicans CaO19.25   likely tRNA guanine methyltransferase similar to S. cerevisiae TRM10 (YOL093W)
Candida albicans CaO19.7696   likely tRNA guanine methyltransferase similar to S. cerevisiae TRM10 (YOL093W)
Caenorhabditis elegans CELE_F25H8.1   Protein F25H8.1, isoform B
Cryptosporidium hominis Chro.50343   hypothetical protein
Cryptosporidium hominis Chro.50384   hypothetical protein
Cryptosporidium parvum cgd5_120   conserved protein
Cryptosporidium parvum cgd5_500   conserved protein
Dictyostelium discoideum DDB_G0274073   hypothetical protein
Dictyostelium discoideum DDB_G0273027   hypothetical protein
Drosophila melanogaster Dmel_CG14618   CG14618 gene product from transcript CG14618-RA
Echinococcus granulosus EgrG_000811500   RNA guanine 9 methyltransferase
Echinococcus granulosus EgrG_000375600   RNA guanine 9 methyltransferase
Entamoeba histolytica EHI_168400   tRNA (guanine-N(1)-)-methyltransferase TRM10
Entamoeba histolytica EHI_106680   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000375600   RNA (guanine 9) methyltransferase
Echinococcus multilocularis EmuJ_000811500   RNA (guanine 9) methyltransferase
Giardia lamblia GL50803_15280   Hypothetical protein
Homo sapiens ENSG00000165275   tRNA methyltransferase 10 homolog B (S. cerevisiae)
Homo sapiens ENSG00000145331   tRNA methyltransferase 10 homolog A (S. cerevisiae)
Leishmania braziliensis LbrM.30.3060   hypothetical protein, conserved
Leishmania donovani LdBPK_303110.1   tRNA (Guanine-1)-methyltransferase, putative
Leishmania infantum LinJ.30.3110   hypothetical protein, conserved
Leishmania major LmjF.30.3070   hypothetical protein, conserved
Leishmania mexicana LmxM.29.3070   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05498   hypothetical protein
Mus musculus ENSMUSG00000004127   tRNA methyltransferase 10A
Mus musculus ENSMUSG00000035601   tRNA methyltransferase 10B
Neospora caninum NCLIV_057660   tRNA (guanine-N1)-methyltransferase domain containing protein, putative
Oryza sativa 4330585   Os02g0725600
Onchocerca volvulus OVOC10417   tRNA methyltransferase 10 homolog
Plasmodium berghei PBANKA_0929000   tRNA m(1)G methyltransferase, putative
Plasmodium falciparum PF3D7_1119100   tRNA m(1)G methyltransferase, putative
Plasmodium knowlesi PKNH_0916800   tRNA m(1)G methyltransferase, putative
Plasmodium vivax PVX_091590   tRNA methyltransferase, putative
Plasmodium yoelii PY04675   hypothetical protein
Saccharomyces cerevisiae YOL093W   Trm10p
Schistosoma japonicum Sjp_0063570   RNA, putative
Schistosoma mansoni Smp_181090   rna (guanine-9-) methyltransferase domain containing
Schmidtea mediterranea mk4.007166.01   tRNA methyltransferase 10 homolog A
Trypanosoma brucei Tb927.6.4420   tRNA (Guanine-1)-methyltransferase, putative
Trypanosoma congolense TcIL3000_6_3830   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504027.10   tRNA (Guanine-1)-methyltransferase, putative
Trypanosoma cruzi TcCLB.509167.170   tRNA (Guanine-1)-methyltransferase, putative
Toxoplasma gondii TGME49_314770   tRNA (guanine-N1)-methyltransferase
Theileria parva TP04_0062   hypothetical protein, conserved
Theileria parva TP04_0533   hypothetical protein
Trichomonas vaginalis TVAG_380400   RNA (guanine-9-) methyltransferase domain containing protein, putative

Essentiality

PF3D7_1119100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.4420 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.4420 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.4420 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.4420 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0929000 Plasmodium berghei Dispensable plasmo
TGME49_314770 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier PF3D7_1119100 (Plasmodium falciparum), tRNA m(1)G methyltransferase, putative
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