Detailed view for Tb927.8.980

Basic information

TDR Targets ID: 16691
Trypanosoma brucei, phosphoacetylglucosamine mutase
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2338 | Length (AA): 602 | MW (Da): 64951 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00408   Phosphoglucomutase/phosphomannomutase, C-terminal domain
PF02878   Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I

Gene Ontology

Mouse over links to read term descriptions.
GO:0071704   GO:organic substance metabolic process  

GO:0016868   intramolecular transferase activity, phosphotransferases  
GO:0005575   cellular_component  
GO:0004610   phosphoacetylglucosamine mutase activity  
GO:0005975   carbohydrate metabolic process  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 597 4bju (A) 1 538 37.00 0 1 1.30243 0.09
31 596 2dka (A) 24 544 40.00 0 1 1.2458 -0.03
499 602 1wjw (A) 5 100 52.00 0 1 0.608257 0.82

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128133)

Species Accession Gene Product
Arabidopsis thaliana AT5G18070   phosphoacetylglucosamine mutase
Babesia bovis BBOV_III000660   phosphoglucomutase, putative
Brugia malayi Bm1_37950   Phosphoglucomutase/phosphomannomutase, C-terminal domain containing protein
Candida albicans CaO19.12480   phosphoacetylglucosamine mutase
Candida albicans CaO19.5013   phosphoacetylglucosamine mutase
Caenorhabditis elegans CELE_F21D5.1   Protein F21D5.1
Cryptosporidium hominis Chro.40374   hypothetical protein
Cryptosporidium parvum cgd4_3310   phosphoacetyl glucosamine mutase
Drosophila melanogaster Dmel_CG10627   nesthocker
Echinococcus granulosus EgrG_000613800   phosphoacetylglucosamine mutase
Echinococcus multilocularis EmuJ_000613800   phosphoacetylglucosamine mutase
Homo sapiens ENSG00000013375   phosphoglucomutase 3
Leishmania braziliensis LbrM.04.0010   phosphoacetylglucosamine mutase-like gene
Leishmania donovani LdBPK_070930.1   phosphoacetylglucosamine mutase-like protein
Leishmania infantum LinJ.07.0930   phosphoacetylglucosamine mutase-like protein,acetylglucosaminephosphomutase, putative,N-acetylglucosamine-phosphate mutase, puta
Leishmania major LmjF.07.0805   phosphoacetylglucosamine mutase-like protein,acetylglucosaminephosphomutase, putative,N-acetylglucosamine-phosphate mutase, puta
Leishmania mexicana LmxM.07.0805   phosphoacetylglucosamine mutase-like protein,acetylglucosaminephosphomutase, putative,N-acetylglucosamine-phosphate mutase, puta
Loa Loa (eye worm) LOAG_03926   phosphoglucomutase/phosphomannomutase domain-containing protein
Mus musculus 109785   phosphoglucomutase 3
Neospora caninum NCLIV_040500   GK12616, related
Oryza sativa 4342646   Os07g0195400
Onchocerca volvulus OVOC4292  
Plasmodium berghei PBANKA_0918200   phosphoacetylglucosamine mutase, putative
Plasmodium falciparum PF3D7_1130000   phosphoacetylglucosamine mutase, putative
Plasmodium knowlesi PKNH_0927900   phosphoacetylglucosamine mutase, putative
Plasmodium vivax PVX_092110   phosphoacetylglucosamine mutase, putative
Plasmodium yoelii PY02130   phosphoacetylglucosamine mutase
Saccharomyces cerevisiae YEL058W   phosphoacetylglucosamine mutase PCM1
Schistosoma japonicum Sjp_0300980   ko:K01836 phosphoacetylglucosamine mutase [EC5.4.2.3], putative
Schistosoma mansoni Smp_157710   phosphoglucomutase
Schmidtea mediterranea mk4.000120.03   Phosphoacetylglucosamine mutase
Schmidtea mediterranea mk4.000120.11   Phosphoacetylglucosamine mutase
Schmidtea mediterranea mk4.000120.10   Phosphoacetylglucosamine mutase
Schmidtea mediterranea mk4.000120.06   Phosphoacetylglucosamine mutase
Schmidtea mediterranea mk4.000120.04  
Schmidtea mediterranea mk4.000120.00   Phosphoacetylglucosamine mutase
Schmidtea mediterranea mk4.000120.05   Phosphoacetylglucosamine mutase
Trypanosoma brucei gambiense Tbg972.8.560   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.980   phosphoacetylglucosamine mutase
Trypanosoma congolense TcIL3000_8_540   phosphoacetylglucosamine mutase, putative
Trypanosoma congolense TcIL3000_0_24070   phosphoacetylglucosamine mutase, putative
Trypanosoma cruzi TcCLB.508569.80   phosphoacetylglucosamine mutase, putative
Trypanosoma cruzi TcCLB.503733.70   phosphoacetylglucosamine mutase, putative
Toxoplasma gondii TGME49_264650   phosphoacetylglucosamine mutase
Theileria parva TP02_0925   N-acetylglucosamine-phosphate mutase, putative

Essentiality

Tb927.8.980 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.980 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.980 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.980 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.980 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F21D5.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_F21D5.1 Caenorhabditis elegans sterile wormbase
YEL058W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0918200 Plasmodium berghei Slow plasmo
TGME49_264650 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.8.980 (Trypanosoma brucei), phosphoacetylglucosamine mutase
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