Detailed view for Tb927.7.5640

Basic information

TDR Targets ID: 16712
Trypanosoma brucei, G-patch domain containing protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.3854 | Length (AA): 271 | MW (Da): 29168 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01585   G-patch domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
130 260 5szh (A) 532 671 12.00 0.17 0 0.625795 -0.69

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128929)

Species Accession Gene Product
Candida albicans CaO19.3831   Possible telomerase regulator or RNA-binding protein
Candida albicans CaO19.11312   Possible telomerase regulator or RNA-binding protein
Caenorhabditis elegans CELE_T23G7.3   Protein T23G7.3
Cryptosporidium hominis Chro.50372   hypothetical protein
Cryptosporidium parvum cgd5_240   G patch domain containing protein
Dictyostelium discoideum DDB_G0276189   hypothetical protein
Dictyostelium discoideum DDB_G0278987   hypothetical protein
Drosophila melanogaster Dmel_CG11180   CG11180 gene product from transcript CG11180-RB
Echinococcus granulosus EgrG_001009700   D111 G patch
Echinococcus multilocularis EmuJ_000132700   G patch domain containing protein 4
Leishmania braziliensis LbrM.06.1000   hypothetical protein, conserved
Leishmania donovani LdBPK_061060.1   G-patch domain containing protein, putative
Leishmania infantum LinJ.06.1060   hypothetical protein, conserved
Leishmania major LmjF.06.1020   hypothetical protein, conserved
Leishmania mexicana LmxM.06.1020   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_11082   hypothetical protein
Mus musculus ENSMUSG00000021958   PIN2/TERF1 interacting, telomerase inhibitor 1
Oryza sativa 4344309   Os07g0682000
Plasmodium berghei PBANKA_0937300   conserved Plasmodium protein, unknown function
Plasmodium knowlesi PKNH_0908000   conserved Plasmodium protein, unknown function
Plasmodium vivax PVX_091165   hypothetical protein, conserved
Plasmodium yoelii PY03703   G-patch domain, putative
Saccharomyces cerevisiae YGR280C   Pxr1p
Schistosoma japonicum Sjp_0092550   ko:K09270 transcription factor SOX7/8/9/10/18 (SOX group E/F), putative
Schistosoma mansoni Smp_010140.3   hypothetical protein
Schistosoma mansoni Smp_010140.2   hypothetical protein
Trypanosoma brucei gambiense Tbg972.7.6540   hypothetical protein, conserved
Trypanosoma brucei Tb927.7.5640   G-patch domain containing protein, putative
Trypanosoma congolense TcIL3000_7_4610   G-patch domain containing protein, putative
Trypanosoma cruzi TcCLB.507559.40   G-patch domain containing protein, putative
Trypanosoma cruzi TcCLB.510303.90   G-patch domain containing protein, putative

Essentiality

Tb927.7.5640 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.5640 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.5640 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.5640 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.5640 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
YGR280C Saccharomyces cerevisiae inviable yeastgenome
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.7.5640 (Trypanosoma brucei), G-patch domain containing protein, putative
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