Detailed view for Tb927.10.9900

Basic information

TDR Targets ID: 16780
Trypanosoma brucei, ABC1 protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.5206 | Length (AA): 515 | MW (Da): 57215 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03109   ABC1 family

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
167 561 4ped (A) 259 640 42.00 0 1 1.27515 -1.25
170 560 4ped (A) 262 639 45.00 0 1 1.31616 -1.32

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127778)

Species Accession Gene Product
Arabidopsis thaliana AT4G01660   ABC transporter 1
Babesia bovis BBOV_IV000470   ABC1family protein, putative
Brugia malayi Bm1_02730   chaperone-activity of bc1 complex-like, mitochondrial, putative
Candida albicans CaO19.3331   similar to S. cerevisiae mitochondrial chaperonin ABC1 (YGL119W) involved in electron transport
Candida albicans CaO19.10842   similar to S. cerevisiae mitochondrial chaperonin ABC1 (YGL119W) involved in electron transport
Caenorhabditis elegans CELE_C35D10.4   Protein COQ-8
Cryptosporidium hominis Chro.80377   ABC1 family
Cryptosporidium parvum cgd8_3250   ABC1 like protein kinase
Dictyostelium discoideum DDB_G0288749   ABC1 family protein kinase
Drosophila melanogaster Dmel_CG32649   CG32649 gene product from transcript CG32649-RA
Echinococcus granulosus EgrG_000395100   ABC domain containing protein kinase
Echinococcus multilocularis EmuJ_000395100   ABC domain containing protein kinase
Homo sapiens ENSG00000163050   aarF domain containing kinase 3
Homo sapiens ENSG00000123815   aarF domain containing kinase 4
Leishmania braziliensis LbrM.35.4770   ABC1 protein, putative
Leishmania donovani LdBPK_364750.1   ABC1 protein, putative
Leishmania infantum LinJ.36.4750   ABC1 protein, putative
Leishmania major LmjF.36.4530   ABC1 protein, putative
Mycobacterium leprae ML0640c   Probable conserved ATP-binding protein ABC transporter
Mus musculus ENSMUSG00000003762   aarF domain containing kinase 4
Mus musculus ENSMUSG00000026489   aarF domain containing kinase 3
Mycobacterium tuberculosis Rv3197   Probable conserved ATP-binding protein ABC transporter
Mycobacterium ulcerans MUL_2510   ABC transporter ATP-binding protein
Neospora caninum NCLIV_021150   ABC1 domain-containing protein, putative
Oryza sativa 4327271   Os01g0318700
Plasmodium berghei PBANKA_1427300   ABC1 family, putative
Plasmodium falciparum PF3D7_0810200   ABC1 family, putative
Plasmodium knowlesi PKNH_1430500   ABC1 family, putative
Plasmodium vivax PVX_123265   ABC1 family, putative
Plasmodium yoelii PY01655   ABC1 family, putative
Saccharomyces cerevisiae YGL119W   Coq8p
Schistosoma japonicum Sjp_0038550   ko:K08869 aarF domain-containing kinase, putative
Schistosoma mansoni Smp_021330.2   ABC transporter
Schistosoma mansoni Smp_021330.1   ABC transporter
Schmidtea mediterranea mk4.000062.08   Chaperone activity of bc1 complex-like, mitochondrial
Schmidtea mediterranea mk4.000062.10  
Trypanosoma brucei gambiense Tbg972.10.12090   ABC1 protein, putative
Trypanosoma brucei Tb927.10.9900   ABC1 protein, putative
Trypanosoma congolense TcIL3000_10_8460   ABC1 protein, putative
Trypanosoma cruzi TcCLB.503847.90   ABC1 protein, putative
Trypanosoma cruzi TcCLB.504103.100   ABC1 protein, putative
Toxoplasma gondii TGME49_203940   ABC1 protein
Theileria parva TP01_0065   ABC1 protein, putative

Essentiality

Tb927.10.9900 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3250 Mycobacterium tuberculosis non-essential nmpdr
Tb927.10.9900 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.9900 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.9900 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.9900 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C35D10.4 Caenorhabditis elegans slow growth wormbase
TGME49_203940 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens aarF domain containing kinase 4 Compounds References
Homo sapiens aarF domain containing kinase 3 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.10.9900 (Trypanosoma brucei), ABC1 protein, putative
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