Detailed view for Tb927.3.2290

Basic information

TDR Targets ID: 16821
Trypanosoma brucei, chaperone protein DnaJ, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2656 | Length (AA): 373 | MW (Da): 43864 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00226   DnaJ domain
PF12171   Zinc-finger double-stranded RNA-binding

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0051082   unfolded protein binding  
GO:0031072   heat shock protein binding  
GO:0003676   nucleic acid binding  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 151 3apq (A) 34 173 38.00 0.0000000033 1 0.82714 -0.24
9 81 1hdj (A) 4 74 52.00 0 1 0.89371 -1.48
9 76 2ctq (A) 22 88 40.00 0.000079 1 0.826706 -1.6
167 275 5cwf (A) 66 172 20.00 0 0.14 0.647225 -2.02

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128332)

Species Accession Gene Product
Arabidopsis thaliana AT1G74250   DNAJ heat shock N-terminal domain-containing protein
Babesia bovis BBOV_IV006710   DnaJ domain containing protein
Brugia malayi Bm1_15665   DnaJ domain containing protein
Candida albicans CaO19.9935   DnaJ-like protein with two potential C2H2 Zn fingers similar to S. cerevisiae JJJ1 (YNL227C)
Candida albicans CaO19.2399   DnaJ-like protein with two potential C2H2 Zn fingers similar to S. cerevisiae JJJ1 (YNL227C)
Caenorhabditis elegans CELE_T03F6.2   Protein DNJ-17
Cryptosporidium hominis Chro.50078   hypothetical protein
Cryptosporidium parvum cgd5_2950   DNAj domain protein
Dictyostelium discoideum DDB_G0286579   hypothetical protein
Drosophila melanogaster Dmel_CG2790   CG2790 gene product from transcript CG2790-RA
Echinococcus granulosus EgrG_000180500   dnaJ subfamily C 1
Entamoeba histolytica EHI_188830   DnaJ domain containing protein
Echinococcus multilocularis EmuJ_000180500   dnaJ subfamily C 1
Giardia lamblia GL50803_16406   Chaperone protein dnaJ
Homo sapiens ENSG00000168724   DnaJ (Hsp40) homolog, subfamily C, member 21
Leishmania braziliensis LbrM.25.1770   hypothetical protein, conserved
Leishmania donovani LdBPK_252290.1   DnaJ domain/Zinc-finger double-stranded RNA-binding, putative
Leishmania infantum LinJ.25.2290   hypothetical protein, conserved
Leishmania major LmjF.25.2190   hypothetical protein, conserved
Leishmania mexicana LmxM.25.2190   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_11616   hypothetical protein
Mus musculus ENSMUSG00000044224   DnaJ (Hsp40) homolog, subfamily C, member 21
Neospora caninum NCLIV_012800   F14N23.23, related
Oryza sativa 4352284   Os12g0502700
Plasmodium berghei PBANKA_1203800   DnaJ protein, putative
Plasmodium falciparum PF3D7_1005600   DnaJ protein, putative
Plasmodium knowlesi PKNH_0804300   DnaJ protein, putative
Plasmodium vivax PVX_094470   hypothetical protein
Plasmodium yoelii PY01286   DnaJ domain, putative
Saccharomyces cerevisiae YNL227C   Jjj1p
Schistosoma japonicum Sjp_0072370   ko:K09506 DnaJ homolog, subfamily A, member 5, putative
Schistosoma mansoni Smp_172510.2   DNAj-related
Schistosoma mansoni Smp_172510.1   DNAj-related
Schmidtea mediterranea mk4.002534.03   DnaJ homolog subfamily C member 21
Trypanosoma brucei gambiense Tbg972.3.2270   chaperone protein DNAj, putative
Trypanosoma brucei Tb927.3.2290   chaperone protein DnaJ, putative
Trypanosoma congolense TcIL3000_0_42300   chaperone protein DnaJ, putative
Trypanosoma cruzi TcCLB.508479.280   hypothetical protein, conserved
Toxoplasma gondii TGME49_301340   DnaJ domain-containing protein
Trichomonas vaginalis TVAG_273650   J protein type, putative
Trichomonas vaginalis TVAG_413330   protein CAJ1, putative

Essentiality

Tb927.3.2290 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.2290 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.2290 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.2290 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.2290 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T03F6.2 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_1203800 Plasmodium berghei Dispensable plasmo
TGME49_301340 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.3.2290 (Trypanosoma brucei), chaperone protein DnaJ, putative
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