Detailed view for Tb927.10.9840

Basic information

TDR Targets ID: 16893
Trypanosoma brucei, chaperone protein DnaJ, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.0458 | Length (AA): 262 | MW (Da): 29824 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00226   DnaJ domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0051082   unfolded protein binding  
GO:0031072   heat shock protein binding  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 68 2lo1 (A) 2 69 53.00 0 1 1.05494 -1.73
3 68 3ucs (C) 2 69 41.00 0 1 0.956208 -2.51
4 68 2och (A) 4 68 52.00 0 1 1.08739 -2.46
5 65 2qsa (A) 35 102 38.00 0.0076 1 0.826224 -2.31
84 228 3q5i (A) 345 499 26.00 0.089 1 0.802835 -0.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128469)

Species Accession Gene Product
Arabidopsis thaliana AT5G06910   chaperone protein dnaJ 6
Arabidopsis thaliana AT3G12170   chaperone DnaJ-domain containing protein
Babesia bovis BBOV_II003490   DnaJ protein, putative
Brugia malayi Bm1_42950   DnaJ domain containing protein
Candida albicans CaO19.2875   DnaJ-like protein similar to S. pombe SPAC1071.09c
Candida albicans CaO19.10393   DnaJ-like protein similar to S. pombe SPAC1071.09c
Caenorhabditis elegans CELE_T24H10.3   Protein DNJ-23
Cryptosporidium hominis Chro.60153   hypothetical protein
Cryptosporidium parvum cgd6_1190   DNAJ protein-like, putative
Dictyostelium discoideum DDB_G0267526   hypothetical protein
Drosophila melanogaster Dmel_CG6693   CG6693 gene product from transcript CG6693-RA
Echinococcus granulosus EgrG_000950900   dnaj subfamily c
Echinococcus multilocularis EmuJ_000950900   dnaj subfamily c
Homo sapiens 23234   DnaJ (Hsp40) homolog, subfamily C, member 9
Leishmania braziliensis LbrM.35.4710   chaperone protein DNAj, putative
Leishmania donovani LdBPK_364690.1   chaperone protein DnaJ, putative
Leishmania infantum LinJ.36.4690   chaperone protein DNAj, putative
Leishmania major LmjF.36.4470   chaperone protein DNAj, putative
Leishmania mexicana LmxM.36.4470   chaperone protein DNAj, putative
Loa Loa (eye worm) LOAG_10225   DnaJ domain-containing protein
Mus musculus ENSMUSG00000021811   DnaJ (Hsp40) homolog, subfamily C, member 9
Neospora caninum NCLIV_058360   hypothetical protein
Oryza sativa 4328611   Os02g0195800
Plasmodium berghei PBANKA_1336400   DnaJ protein, putative
Plasmodium falciparum PF3D7_1473200   DnaJ protein, putative
Plasmodium knowlesi PKNH_1207800   DnaJ protein, putative
Plasmodium vivax PVX_116820   DnaJ domain containing protein
Plasmodium yoelii PY03688   5702-7336, putative
Schistosoma japonicum Sjp_0011820   ko:K09529 DnaJ homolog, subfamily C, member 9, putative
Schistosoma mansoni Smp_083450   DNAj homolog subfamily C member
Schmidtea mediterranea mk4.002768.00   DnaJ homolog subfamily C member 9
Schmidtea mediterranea mk4.004545.04   DnaJ homolog subfamily C member 9
Trypanosoma brucei gambiense Tbg972.10.12010   chaperone protein DNAj, putative
Trypanosoma brucei Tb927.10.9840   chaperone protein DnaJ, putative
Trypanosoma congolense TcIL3000_10_8530   chaperone protein DnaJ, putative
Trypanosoma cruzi TcCLB.504147.60   chaperone DNAJ protein, putative
Toxoplasma gondii TGME49_315690   DnaJ domain-containing protein
Theileria parva TP04_0235   hypothetical protein, conserved

Essentiality

Tb927.10.9840 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.9840 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.9840 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.9840 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.9840 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_315690 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.9840 (Trypanosoma brucei), chaperone protein DnaJ, putative
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