Detailed view for Tb927.4.1860

Basic information

TDR Targets ID: 17152
Trypanosoma brucei, ribosomal protein S19, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 11.3629 | Length (AA): 167 | MW (Da): 18846 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01090   Ribosomal protein S19e

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
21 167 2v7f (A) 2 148 31.00 0 1 1.32974 -1.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3ZEY:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127080)

Species Accession Gene Product
Arabidopsis thaliana AT3G02080   40S ribosomal protein S19-1
Arabidopsis thaliana AT5G61170   40S ribosomal protein S19-3
Arabidopsis thaliana AT5G15520   40S ribosomal protein S19-2
Babesia bovis BBOV_III001110   40S ribosomal protein S19, putative
Brugia malayi Bm1_18840   40S ribosomal protein S19S
Brugia malayi Bm1_57295   40S ribosomal protein S19S
Caenorhabditis elegans CELE_T05F1.3   Protein RPS-19
Cryptosporidium hominis Chro.10106   hypothetical protein
Cryptosporidium parvum cgd1_850   40S ribosomal protein S19
Dictyostelium discoideum DDB_G0279207   40S ribosomal protein S19
Drosophila melanogaster Dmel_CG4464   Ribosomal protein S19a
Drosophila melanogaster Dmel_CG5338   Ribosomal protein S19b
Echinococcus granulosus EgrG_000480600   ribosomal protein S19e
Entamoeba histolytica EHI_198740   40S ribosomal protein S19, putative
Entamoeba histolytica EHI_146570   40S ribosomal protein S19, putative
Echinococcus multilocularis EmuJ_000480600   ribosomal protein S19e
Homo sapiens 6223   ribosomal protein S19
Leishmania braziliensis LbrM.20.2340   40S ribosomal protein S19 protein, putative
Leishmania braziliensis LbrM.03.0920   40S ribosomal protein S19 protein
Leishmania donovani LdBPK_342620.1   40S ribosomal protein S19 protein, putative
Leishmania infantum LinJ.34.2620   40S ribosomal protein S19 protein, putative
Leishmania infantum LinJ.29.2970   40S ribosomal protein S19-like protein
Leishmania major LmjF.34.2780   40S ribosomal protein S19 protein, putative
Leishmania major LmjF.29.2860   40S ribosomal protein S19-like protein
Leishmania mexicana LmxM.08_29.2860   40S ribosomal protein S19-like protein
Leishmania mexicana LmxM.33.2780   40S ribosomal protein S19 protein, putative
Loa Loa (eye worm) LOAG_01581   40S ribosomal protein S19S
Loa Loa (eye worm) LOAG_04829   40S ribosomal protein S19S
Mus musculus ENSMUSG00000040952   ribosomal protein S19
Neospora caninum NCLIV_049100   Ribosomal protein S19e, related
Oryza sativa 4333150   Os03g0424800
Oryza sativa 4333148   Os03g0424500
Onchocerca volvulus OVOC1707   40S ribosomal protein S19 homolog
Onchocerca volvulus OVOC776   40S ribosomal protein S19 homolog
Plasmodium berghei PBANKA_0522800   40S ribosomal protein S19, putative
Plasmodium falciparum PF3D7_0422400   40S ribosomal protein S19
Plasmodium knowlesi PKNH_0514300   40S ribosomal protein S19, putative
Plasmodium vivax PVX_090160   40S ribosomal protein S19, putative
Plasmodium yoelii PY04062   Ribosomal protein S19e, putative
Saccharomyces cerevisiae YNL302C   ribosomal 40S subunit protein S19B
Saccharomyces cerevisiae YOL121C   ribosomal 40S subunit protein S19A
Schistosoma japonicum Sjp_0034480   ko:K02966 small subunit ribosomal protein S19e, putative
Schistosoma mansoni Smp_174950   40S ribosomal protein S19
Schmidtea mediterranea mk4.001523.14   40S ribosomal protein S19
Schmidtea mediterranea mk4.024113.00   40S ribosomal protein S19
Schmidtea mediterranea mk4.017924.00   40S ribosomal protein S19
Schmidtea mediterranea mk4.010902.02   40S ribosomal protein S19
Schmidtea mediterranea mk4.010092.01   40S ribosomal protein S19
Trypanosoma brucei gambiense Tbg972.4.1770   ribosomal protein S19, putative
Trypanosoma brucei Tb927.4.1860   ribosomal protein S19, putative
Trypanosoma congolense TcIL3000_4_1520   ribosomal protein S19, putative
Trypanosoma cruzi TcCLB.504013.100   ribosomal protein S19, putative
Trypanosoma cruzi TcCLB.510879.20   ribosomal protein S19, putative
Toxoplasma gondii TGME49_212290   ribosomal protein RPS19
Theileria parva TP03_0716   40S ribosomal protein S19, putative
Trichomonas vaginalis TVAG_352580   40S ribosomal protein S19, putative
Trichomonas vaginalis TVAG_199240   40S ribosomal protein S19, putative
Trichomonas vaginalis TVAG_352560   40S ribosomal protein S19, putative
Trichomonas vaginalis TVAG_207570   40S ribosomal protein S19, putative
Trichomonas vaginalis TVAG_352520   40S ribosomal protein S19, putative
Trichomonas vaginalis TVAG_352600   40S ribosomal protein S19, putative
Trichomonas vaginalis TVAG_353280   40S ribosomal protein S19, putative

Essentiality

Tb927.4.1860 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.1860 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.1860 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.1860 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.1860 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T05F1.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_T05F1.3 Caenorhabditis elegans larval arrest wormbase
CELE_T05F1.3 Caenorhabditis elegans sterile wormbase
TGME49_212290 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.4.1860 (Trypanosoma brucei), ribosomal protein S19, putative
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