Detailed view for PF3D7_1124600

Basic information

TDR Targets ID: 1726
Plasmodium falciparum, ethanolamine kinase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 5.5537 | Length (AA): 423 | MW (Da): 49914 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01633   Choline/ethanolamine kinase

Gene Ontology

Mouse over links to read term descriptions.
GO:0006656   phosphatidylcholine biosynthetic process  
GO:0005575   cellular_component  
GO:0004305   ethanolamine kinase activity  
GO:0006646   phosphatidylethanolamine biosynthetic process  

Metabolic Pathways

Glycerophospholipid metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
66 422 1nw1 (A) 35 424 25.00 0 1 1.06 -1.16
49 420 2qg7 (A) 50 458 69.00 0 1 1.81073 -1.68

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2QG7:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, merozoite, early schizont, early trophozoite, late schizont, late trophozoite, Oocyst. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, gametocyte, sporozoite, late ring, Ring, Sporozoite, Female Gametocyte, Male Gametocyte. Otto TD PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 16 hs, early ring. Otto TD PlasmoDB
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127649)

Species Accession Gene Product
Arabidopsis thaliana AT2G26830   protein embryo defective 1187
Babesia bovis BBOV_IV008320   choline/ethanolamine kinase, putative
Babesia bovis BBOV_IV008310   choline/ethanolamine kinase, putative
Brugia malayi Bm1_37000   Choline/ethanolamine kinase family protein
Candida albicans CaO19.6912   similar to ethanolamine kinase
Candida albicans CaO19_6912   hypothetical protein
Caenorhabditis elegans CELE_T27A10.3   Protein CKC-1, isoform B
Cryptosporidium hominis Chro.50319   choline/ethanolamine kinase
Cryptosporidium parvum cgd5_720   choline/ethanolamine kinase family protein
Dictyostelium discoideum DDB_G0274955   ethanolamine kinase A
Dictyostelium discoideum DDB_G0275265   ethanolamine kinase B
Drosophila melanogaster Dmel_CG3525   easily shocked
Echinococcus granulosus EgrG_000360100   ethanolamine kinase 2
Entamoeba histolytica EHI_152340   choline/ethanolamine kinase, putative
Entamoeba histolytica EHI_148580   choline/ethanolamine kinase, putative
Echinococcus multilocularis EmuJ_000360100   ethanolamine kinase 2
Giardia lamblia GL50803_4596   Ethanolamine kinase, putative
Homo sapiens ENSG00000143845   ethanolamine kinase 2
Homo sapiens ENSG00000139163   ethanolamine kinase 1
Leishmania braziliensis LbrM.27.1560   ethanolamine kinase, putative
Leishmania donovani LdBPK_271330.1   ethanolamine kinase, putative
Leishmania infantum LinJ.27.1330   ethanolamine kinase, putative
Leishmania major LmjF.27.1420   choline kinase
Leishmania mexicana LmxM.27.1420   choline/ethanolamine kinase, putative
Loa Loa (eye worm) LOAG_08101   choline/ethanolamine kinase
Loa Loa (eye worm) LOAG_12651   hypothetical protein
Mus musculus ENSMUSG00000070644   ethanolamine kinase 2
Mus musculus ENSMUSG00000030275   ethanolamine kinase 1
Neospora caninum NCLIV_044730   GmCK2p (EC 2.7.1.32), related
Oryza sativa 4347160   Os09g0438400
Plasmodium berghei PBANKA_0923700   ethanolamine kinase, putative
Plasmodium falciparum PF3D7_1124600   ethanolamine kinase
Plasmodium knowlesi PKNH_0922400   ethanolamine kinase, putative
Plasmodium vivax PVX_091845   ethanolamine kinase, putative
Plasmodium yoelii PY00818   Choline/ethanolamine kinase, putative
Schistosoma japonicum Sjp_0016450   Ethanolamine kinase 1, putative
Schistosoma japonicum Sjp_0202810   ko:K00894 ethanolamine kinase [EC2.7.1.82], putative
Schistosoma mansoni Smp_015050   choline kinase
Schistosoma mansoni Smp_015030   choline/ethanolamine kinase
Schmidtea mediterranea mk4.000069.07  
Schmidtea mediterranea mk4.000069.09  
Trypanosoma brucei gambiense Tbg972.11.2280   choline/ethanolamine kinase, putative
Trypanosoma brucei Tb927.11.2090   choline kinase
Trypanosoma cruzi TcCLB.508355.190   choline kinase
Toxoplasma gondii TGME49_306540   phosphotransferase enzyme family protein
Theileria parva TP01_0942   choline kinase, putative
Trichomonas vaginalis TVAG_333190   choline/ethanolamine kinase, putative

Essentiality

PF3D7_1124600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.18.0017 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.18.0017 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.18.0017 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.18.0017 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0923700 Plasmodium berghei Slow plasmo
TGME49_306540 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier PF3D7_1124600 (Plasmodium falciparum), ethanolamine kinase
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