Detailed view for Tb927.7.890

Basic information

TDR Targets ID: 17403
Trypanosoma brucei, electron transfer protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6446 | Length (AA): 178 | MW (Da): 19713 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00111   2Fe-2S iron-sulfur cluster binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0051537   2 iron, 2 sulfur cluster binding  
GO:0051536   iron-sulfur cluster binding  
GO:0009055   electron carrier activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
57 177 5uj5 (A) 15 132 41.00 0 1 1.09408 0.55
58 171 2mjd (A) 2 113 46.00 0 1 1.18275 -0.16
61 161 3lxf (A) 3 102 32.00 0 1 1.04372 -1.02
67 164 1uwm (A) 8 105 38.00 0 1 1.02086 -0.48

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126994)

Species Accession Gene Product
Arabidopsis thaliana AT4G21090   ferredoxin 2
Arabidopsis thaliana AT4G05450   mitochondrial ferredoxin 1
Babesia bovis BBOV_IV001460   adrenodoxin-type ferredoxin, putative
Brugia malayi Bm1_55765   2Fe-2S iron-sulfur cluster binding domain containing protein
Brugia malayi Bm1_36585   Adrenodoxin-like protein, mitochondrial precursor, putative
Candida albicans CaO19.336   oxidoreductase
Candida albicans CaO19.7969   oxidoreductase
Caenorhabditis elegans CELE_Y73F8A.27   Protein Y73F8A.27
Cryptosporidium parvum cgd6_3000   ferredoxin-like protein Fd1, putative
Dictyostelium discoideum DDB_G0267486   hypothetical protein
Drosophila melanogaster Dmel_CG4205   Ferredoxin
Drosophila melanogaster Dmel_CG1319   CG1319 gene product from transcript CG1319-RB
Escherichia coli b2525   [2Fe-2S] ferredoxin
Echinococcus granulosus EgrG_000190000   ferredoxin
Echinococcus granulosus EgrG_000040700   Adrenodoxin mitochondrial
Echinococcus multilocularis EmuJ_000190000   ferredoxin adrenodoxin
Echinococcus multilocularis EmuJ_000040700   Adrenodoxin, mitochondrial
Giardia lamblia GL50803_27266   [2Fe-2S] ferredoxin
Homo sapiens ENSG00000267673   ferredoxin 1-like
Homo sapiens ENSG00000137714   ferredoxin 1
Leishmania braziliensis LbrM.31.2770   ferredoxin 2fe-2s-like protein
Leishmania braziliensis LbrM.26.1070   hypothetical protein, conserved
Leishmania braziliensis LbrM.31.2820   ferredoxin 2fe-2s-like protein
Leishmania donovani LdBPK_312570.1   ferredoxin 2fe-2s-like protein
Leishmania donovani LdBPK_261030.1   2Fe-2S iron-sulfur cluster binding domain containing protein, putative
Leishmania infantum LinJ.31.2570   ferredoxin 2fe-2s-like protein
Leishmania infantum LinJ.26.1030   hypothetical protein, conserved
Leishmania major LmjF.31.2480   ferredoxin 2fe-2s-like protein
Leishmania major LmjF.26.1050   hypothetical protein, conserved
Leishmania mexicana LmxM.30.2480   ferredoxin 2fe-2s-like protein
Leishmania mexicana LmxM.26.1050   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_00128   2Fe-2S iron-sulfur cluster binding domain-containing protein
Mus musculus ENSMUSG00000079677   ferredoxin 1-like
Mus musculus ENSMUSG00000032051   ferredoxin 1
Neospora caninum NCLIV_016870   hypothetical protein
Neospora caninum NCLIV_050330   2Fe-2S iron-sulfur cluster binding domain containing protein, putative
Oryza sativa 4342226   Os07g0110300
Oryza sativa 4347157   Os09g0437900
Onchocerca volvulus OVOC4595  
Onchocerca volvulus OVOC705   Adrenodoxin, mitochondrial homolog
Plasmodium berghei PBANKA_1430400   adrenodoxin-type ferredoxin, putative
Plasmodium falciparum PF3D7_1214600   adrenodoxin-type ferredoxin, putative
Plasmodium knowlesi PKNH_1434100   adrenodoxin-type ferredoxin, putative
Plasmodium yoelii PY07468   Adrenodoxin precursor
Saccharomyces cerevisiae YPL252C   Yah1p
Schistosoma japonicum Sjp_0037340   ko:K04755 ferredoxin, 2Fe-2S, putative
Schistosoma japonicum Sjp_0028320   Adrenodoxin, mitochondrial precursor, putative
Schistosoma mansoni Smp_124940   adrenodoxin
Schistosoma mansoni Smp_016910   adrenodoxin
Schmidtea mediterranea mk4.039500.00  
Schmidtea mediterranea mk4.000884.02  
Trypanosoma brucei gambiense Tbg972.7.840   electron transfer protein, putative
Trypanosoma brucei Tb927.4.4980   NADH-ubiquinone oxidoreductase complex I subunit, putative
Trypanosoma brucei Tb927.7.890   electron transfer protein, putative
Trypanosoma congolense TcIL3000_7_480   electron transfer protein, putative
Trypanosoma congolense TcIL3000_0_52800   electron transfer protein, putative
Trypanosoma congolense TcIL3000_8_7670   adrenodoxin precursor, putative
Trypanosoma congolense TcIL3000_4_4210   adrenodoxin precursor, putative
Trypanosoma cruzi TcCLB.508879.70   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.504575.40   adrenodoxin precursor, putative
Trypanosoma cruzi TcCLB.421057.10   adrenodoxin precursor, putative
Trypanosoma cruzi TcCLB.509011.70   adrenodoxin precursor, putative
Toxoplasma gondii TGME49_236000   ferredoxin, putative
Toxoplasma gondii TGME49_240670   adrenodoxin-type ferredoxin, putative
Theileria parva TP01_0173   adrenodoxin-type ferredoxin, putative
Trichomonas vaginalis TVAG_078730   Ferredoxin 7
Wolbachia endosymbiont of Brugia malayi Wbm0507   ferredoxin

Essentiality

Tb927.7.890 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.890 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.7.890 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.890 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.890 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.4.4980 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.4980 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.4980 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.4980 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y73F8A.27 Caenorhabditis elegans embryonic arrest wormbase
YPL252C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1430400 Plasmodium berghei Essential plasmo
TGME49_240670 Toxoplasma gondii Probably non-essential sidik
TGME49_236000 Toxoplasma gondii Probably non-essential sidik
TGME49_240670 Toxoplasma gondii Probably essential sidik
TGME49_236000 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.7.890 (Trypanosoma brucei), electron transfer protein, putative
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