Detailed view for Tb927.11.14350

Basic information

TDR Targets ID: 17456
Trypanosoma brucei, mitochondrial carrier protein
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.7132 | Length (AA): 346 | MW (Da): 38740 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 2

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00153   Mitochondrial carrier protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005743   mitochondrial inner membrane  
GO:0005488   binding  
GO:0006810   transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127110)

Species Accession Gene Product
Arabidopsis thaliana AT1G25380   NAD+ transporter 2
Arabidopsis thaliana AT2G47490   NAD+ transporter 1
Arabidopsis thaliana AT5G66380   folate transporter 1
Babesia bovis BBOV_III006870   mitochondrial carrier protein, putative
Brugia malayi Bm1_20990   Mitochondrial carrier protein
Candida albicans CaO19_6532   hypothetical protein
Candida albicans CaO19.8971   likely mitochondrial carrier protein similar to S. cerevisiae YIL006W
Candida albicans CaO19.1393   likely mitochondrial carrier protein similar to S. cerevisiae YIL006W
Candida albicans CaO19.6532   likely mitochondrial carrier protein similar to S. cerevisiae FLX1 (YIL134W) FAD carrier protein
Candida albicans CaO19.13885   likely mitochondrial carrier protein similar to S. cerevisiae FLX1 (YIL134W) FAD carrier protein
Caenorhabditis elegans CELE_K01C8.7   Protein K01C8.7, isoform B
Cryptosporidium hominis Chro.10266   ENSANGP00000009305
Cryptosporidium parvum cgd1_2370   mitochondrial carrier protein, Flx1p like mitochondrial membrane associated flavin transporter with 4 or more transmembrane doma
Dictyostelium discoideum DDB_G0290669   mitochondrial substrate carrier family protein
Dictyostelium discoideum DDB_G0283751   mitochondrial substrate carrier family protein
Drosophila melanogaster Dmel_CG8026   CG8026 gene product from transcript CG8026-RD
Echinococcus granulosus EgrG_000964400   mitochondrial folate transporter:carrier
Echinococcus multilocularis EmuJ_000964400   mitochondrial folate transporter:carrier
Homo sapiens ENSG00000164933   solute carrier family 25 (mitochondrial folate carrier), member 32
Leishmania braziliensis LbrM.34.3240   mitochondrial carrier protein, putative
Leishmania braziliensis LbrM.32.1210   mitochondrial carrier protein, putative
Leishmania braziliensis LbrM.32.1220   mitochondrial carrier protein-like protein
Leishmania donovani LdBPK_321180.1   mitochondrial carrier protein-like protein
Leishmania donovani LdBPK_321170.1   mitochondrial carrier protein, putative
Leishmania donovani LdBPK_353380.1   mitochondrial carrier protein, putative
Leishmania infantum LinJ.32.1180   mitochondrial carrier protein-like protein
Leishmania infantum LinJ.35.3380   mitochondrial carrier protein, putative
Leishmania infantum LinJ.32.1170   mitochondrial carrier protein, putative,mitochondrial pyruvate carrier-like protein
Leishmania major LmjF.35.3330   mitochondrial carrier protein, putative
Leishmania major LmjF.32.1110   mitochondrial carrier protein, putative,mitochondrial pyruvate carrier-like protein
Leishmania major LmjF.32.1120   mitochondrial carrier protein-like protein
Leishmania mexicana LmxM.31.1110   mitochondrial carrier protein, putative,mitochondrial pyruvate carrier-like protein
Leishmania mexicana LmxM.31.1120   mitochondrial carrier protein-like protein
Leishmania mexicana LmxM.34.3330   mitochondrial carrier protein, putative
Loa Loa (eye worm) LOAG_04603   hypothetical protein
Mus musculus ENSMUSG00000022299   solute carrier family 25, member 32
Neospora caninum NCLIV_022040   mitochondrial carrier domain-containing protein, putative
Neospora caninum NCLIV_044910   Os03g0734700 protein, related
Oryza sativa 4323984   Os01g0513200
Oryza sativa 9266735   Os01g0513301
Oryza sativa 4334018   Os03g0734700
Oryza sativa 4338524   Os05g0357200
Onchocerca volvulus OVOC2218   Mitochondrial folate transporter\/carrier homolog
Saccharomyces cerevisiae YIL134W   Flx1p
Saccharomyces cerevisiae YIL006W   Yia6p
Saccharomyces cerevisiae YEL006W   Yea6p
Schistosoma japonicum Sjp_0313780   Mitochondrial folate transporter/carrier, putative
Schistosoma mansoni Smp_044020   folate carrier protein
Schmidtea mediterranea mk4.006619.00   Mitochondrial folate transporter/carrier
Schmidtea mediterranea mk4.001995.03   Mitochondrial folate transporter/carrier
Trypanosoma brucei gambiense Tbg972.11.16080   mitochondrial carrier protein, putative
Trypanosoma brucei gambiense Tbg972.9.7360   mitochondrial carrier protein, putative
Trypanosoma brucei gambiense Tbg972.11.16100   mitochondrial carrier protein, putative
Trypanosoma brucei Tb927.11.14350   mitochondrial carrier protein
Trypanosoma brucei Tb927.11.14360   mitochondrial carrier protein
Trypanosoma brucei Tb927.9.12140   mitochondrial carrier protein
Trypanosoma congolense TcIL3000_9_5000   mitochondrial carrier protein, putative
Trypanosoma congolense TcIL3000.11.14690   mitochondrial carrier protein
Trypanosoma congolense TcIL3000.11.14680   mitochondrial carrier protein
Trypanosoma cruzi TcCLB.511725.140   mitochondrial carrier protein, putative
Trypanosoma cruzi TcCLB.510737.30   mitochondrial carrier protein, putative
Trypanosoma cruzi TcCLB.510663.64   mitochondrial carrier protein, putative
Trypanosoma cruzi TcCLB.511725.134   mitochondrial carrier protein, putative
Toxoplasma gondii TGME49_228680   mitochondrial carrier superfamily protein
Theileria parva TP04_0593   hypothetical protein

Essentiality

Tb927.11.14350 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.5950 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.5950 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.5950 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.01.5950 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.01.5960 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.5960 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.5960 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.5960 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb09.211.3200 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.3200 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.3200 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.3200 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_K01C8.7 Caenorhabditis elegans slow growth wormbase
TGME49_228680 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.14350 (Trypanosoma brucei), mitochondrial carrier protein
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