Detailed view for Tb927.10.13710

Basic information

TDR Targets ID: 17485
Trypanosoma brucei, Activator of Hsp90 ATPase, N-terminal/Activator of Hsp90 ATPase homolog 1-like protein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.5092 | Length (AA): 331 | MW (Da): 37612 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08327   Activator of Hsp90 ATPase homolog 1-like protein
PF09229   Activator of Hsp90 ATPase, N-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0051087   chaperone binding  
GO:0001671   ATPase activator activity  
GO:0006950   response to stress  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
21 158 3n72 (A) 11 153 20.00 0 1 0.688718 -0.56
27 158 1usv (B) 11 150 28.00 0.000000016 1 0.791192 -0.95
66 164 5lnl (A) 1610 1714 34.00 0.78 0.02 0.281494 1.67
190 328 1x53 (A) 2 139 31.00 0 1 0.93994 -1.19
200 326 3ni8 (A) 20 154 28.00 0.0000000072 1 0.820086 -1.39

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128037)

Species Accession Gene Product
Arabidopsis thaliana AT3G12050   Aha1 domain-containing protein
Brugia malayi Bm1_50910   activator of 90 kDa heat shock protein ATPase homolog 1
Candida albicans CaO19.7602   similar to S. cerevisiae AHA1 (YDR214W) Hsp90 system cochaperone
Caenorhabditis elegans CELE_C01G10.8   Protein C01G10.8
Cryptosporidium parvum cgd2_320   conserved hypothetical protein
Dictyostelium discoideum DDB_G0269712   activator of Hsp90 ATPase family protein
Drosophila melanogaster Dmel_CG1416   CG1416 gene product from transcript CG1416-RA
Echinococcus granulosus EgrG_000241900   Activator of 90 kDa heat shock protein ATPase
Entamoeba histolytica EHI_065800   Aha1 domain protein, putative
Echinococcus multilocularis EmuJ_000241900   Activator of 90 kDa heat shock protein ATPase
Giardia lamblia GL50803_10423   Hypothetical protein
Homo sapiens ENSG00000100591   AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
Homo sapiens ENSG00000173209   AHA1, activator of heat shock 90kDa protein ATPase homolog 2 (yeast)
Leishmania braziliensis LbrM.18.0230   hypothetical protein, conserved
Leishmania donovani LdBPK_180210.1   Activator of Hsp90 ATPase, N-terminal/Activator of Hsp90 ATPase homolog 1-like protein, putative
Leishmania infantum LinJ.18.0210   hypothetical protein, conserved
Leishmania major LmjF.18.0210   hypothetical protein, conserved
Leishmania mexicana LmxM.18.0210   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_11151   hypothetical protein
Mus musculus ENSMUSG00000020288   AHA1, activator of heat shock protein ATPase 2
Mus musculus ENSMUSG00000021037   AHA1, activator of heat shock protein ATPase 1
Neospora caninum NCLIV_018620   Os08g0464000 protein, related
Oryza sativa 4345780   Os08g0464000
Onchocerca volvulus OVOC7019   Activator of 90 kDa heat shock protein ATPase homolog
Plasmodium berghei PBANKA_0406700   activator of Hsp90 ATPase, putative
Plasmodium falciparum PF3D7_0308500   activator of Hsp90 ATPase, putative
Plasmodium knowlesi PKNH_0834200   activator of Hsp90 ATPase, putative
Plasmodium vivax PVX_119540   activator of Hsp90 ATPase, putative
Saccharomyces cerevisiae YDR214W   Aha1p
Schistosoma japonicum Sjp_0004180   similar to Activator of 90 kDa heat shock protein ATPase homolog 1, putative
Schistosoma mansoni Smp_083080.1   hypothetical protein
Schistosoma mansoni Smp_083080.2   hypothetical protein
Schmidtea mediterranea mk4.001985.03  
Trypanosoma brucei gambiense Tbg972.10.16840   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.13710   Activator of Hsp90 ATPase, N-terminal/Activator of Hsp90 ATPase homolog 1-like protein, putative
Trypanosoma congolense TcIL3000_10_11950   Activator of Hsp90 ATPase, N-terminal/Activator of Hsp90 ATPase homolog 1-like protein, putative
Trypanosoma cruzi TcCLB.507993.150   Activator of Hsp90 ATPase, N-terminal/Activator of Hsp90 ATPase homolog 1-like protein, putative
Trypanosoma cruzi TcCLB.511277.590   Activator of Hsp90 ATPase, N-terminal/Activator of Hsp90 ATPase homolog 1-like protein, putative
Toxoplasma gondii TGME49_244020   activator of hsp90 atpase 1 family protein

Essentiality

Tb927.10.13710 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.13710 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.13710 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.13710 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.13710 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0406700 Plasmodium berghei Slow plasmo
TGME49_244020 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.10.13710 (Trypanosoma brucei), Activator of Hsp90 ATPase, N-terminal/Activator of Hsp90 ATPase homolog 1-like protein, putative
Title for this comment
Comment