Detailed view for Tb927.7.940

Basic information

TDR Targets ID: 17524
Trypanosoma brucei, protein kinase C substrate protein, heavy chain, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.8988 | Length (AA): 481 | MW (Da): 54435 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12999   Glucosidase II beta subunit-like
PF13015   Glucosidase II beta subunit-like protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0006491   N-glycan processing  
GO:0005515   protein binding  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
37 137 5jqp (B) 34 154 56.00 0 1 0.501179 0.49
41 134 5f0e (B) 33 116 57.00 0 0.94 0.609126 0.57
41 80 2i1p (A) 1189 1228 35.00 0 0.15 0.43386 -0.59
135 229 2fxm (B) 866 960 22.00 0 0 0.468205 -1.24

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128657)

Species Accession Gene Product
Arabidopsis thaliana AT5G56360   glucosidase II beta subunit protein PSL4
Brugia malayi Bm1_42055   protein kinase C substrate 80K-H
Brugia malayi Bm1_42060   glucosidase II beta subunit
Candida albicans CaO19.3286   similar to S. cerevisiae YDR221W
Candida albicans CaO19.10796   similar to S. cerevisiae YDR221W
Caenorhabditis elegans CELE_ZK1307.8   Protein ZK1307.8
Dictyostelium discoideum DDB_G0271120   hypothetical protein
Drosophila melanogaster Dmel_CG6453   CG6453 gene product from transcript CG6453-RA
Echinococcus granulosus EgrG_000532800   Glucosidase II beta subunit
Entamoeba histolytica EHI_135420   glucosidase 2 subunit beta precursor, putative
Echinococcus multilocularis EmuJ_000532800   Glucosidase II beta subunit
Homo sapiens ENSG00000130175   protein kinase C substrate 80K-H
Leishmania braziliensis LbrM.26.1010   hypothetical protein, conserved
Leishmania donovani LdBPK_260970.1   glucosidase II beta subunit-like protein, putative
Leishmania infantum LinJ.26.0970   hypothetical protein, conserved
Leishmania major LmjF.26.1000   hypothetical protein, conserved
Leishmania mexicana LmxM.26.1000   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_02299   hypothetical protein
Mus musculus ENSMUSG00000003402   protein kinase C substrate 80K-H
Oryza sativa 4324264   Os01g0276800
Onchocerca volvulus OVOC10792   Glucosidase 2 subunit beta homolog
Saccharomyces cerevisiae YDR221W   Gtb1p
Schistosoma japonicum Sjp_0205040   ko:K08288 protein kinase C substrate 80K-H, putative
Schistosoma mansoni Smp_131400   glucosidase II beta subunit
Schmidtea mediterranea mk4.001111.01   Glucosidase 2 subunit beta
Trypanosoma brucei gambiense Tbg972.7.890   protein kinase C substrate protein, heavy chain, putative,glucosidase II beta subunit, putative
Trypanosoma brucei Tb927.7.940   protein kinase C substrate protein, heavy chain, putative
Trypanosoma congolense TcIL3000_7_580   protein kinase C substrate protein, heavy chain, putative
Trypanosoma cruzi TcCLB.503463.10   protein kinase C substrate protein, heavy chain, putative
Trypanosoma cruzi TcCLB.508879.4   protein kinase C substrate protein, heavy chain, putative
Trypanosoma cruzi TcCLB.508215.9   protein kinase C substrate protein, heavy chain, putative
Trichomonas vaginalis TVAG_014140   N-linked oligosaccharide processing, putative
Trichomonas vaginalis TVAG_151650   glucosidase II beta subunit, putative
Trichomonas vaginalis TVAG_150160   glucosidase II beta subunit, putative
Trichomonas vaginalis TVAG_173790   glucosidase II beta subunit, putative

Essentiality

Tb927.7.940 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.940 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.940 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.940 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.940 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.7.940 (Trypanosoma brucei), protein kinase C substrate protein, heavy chain, putative
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