Detailed view for Tb927.11.3130

Basic information

TDR Targets ID: 17545
Trypanosoma brucei, glycosomal transporter (GAT2)
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.0319 | Length (AA): 641 | MW (Da): 71267 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 4

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00005   ABC transporter
PF06472   ABC transporter transmembrane region 2

Gene Ontology

Mouse over links to read term descriptions.
GO:0043190   ATP-binding cassette (ABC) transporter complex  
GO:0042626   ATPase activity, coupled to transmembrane movement of substances  
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  
GO:0017111   nucleoside-triphosphatase activity  
GO:0016887   ATPase activity  
GO:0006810   transport  
GO:0055085   transmembrane transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
61 631 4q4h (B) 14 569 17.00 0 1 0.988296 0.75
73 636 5mkk (B) 4 557 18.00 0 0.98 1.00138 0.79
421 636 3vx4 (A) 525 746 23.00 0 1 0.653473 -0.74
549 624 2ghi (D) 143 227 37.00 0.0014 0.54 0.333965 0.93

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126726)

Species Accession Gene Product
Arabidopsis thaliana AT4G39850   ABC transporter D family member 1
Arabidopsis thaliana AT1G54350   ABC transporter family protein
Brugia malayi Bm1_08720   ABC transporter N-terminus family protein
Brugia malayi Bm1_01595   hypothetical protein
Brugia malayi Bm1_07815   hypothetical protein
Candida albicans CaO19.5255   potential peroxisomal fatty acid ABC transporter similar to S. cerevisiae PXA2 (YKL188C) ABC transporter 2
Candida albicans CaO19.12720   potential peroxisomal fatty acid ABC transporter similar to S. cerevisiae PXA2 (YKL188C) ABC transporter 2
Candida albicans CaO19.7500   potential peroxisomal ABC transporter similar to S. cerevisiae PXA1 (YPL147W) transporter of long-chain fatty acids
Caenorhabditis elegans CELE_C44B7.8   Protein PMP-1
Caenorhabditis elegans CELE_C44B7.9   Protein PMP-2
Caenorhabditis elegans CELE_C54G10.3   Protein PMP-3, isoform A
Cryptosporidium hominis Chro.70309   ABC transporter ATP-binding protein
Cryptosporidium parvum cgd7_2730   ABC transporter with ATpase domain plus 3 transmembrane regions
Dictyostelium discoideum DDB_G0293194   ABC transporter D family protein
Dictyostelium discoideum DDB_G0279917   ABC transporter D family protein
Dictyostelium discoideum DDB_G0279919   ABC transporter D family protein
Drosophila melanogaster Dmel_CG2316   CG2316 gene product from transcript CG2316-RB
Drosophila melanogaster Dmel_CG12703   CG12703 gene product from transcript CG12703-RD
Escherichia coli b1496   putative multidrug transporter subunit of ABC superfamily, membrane component/ATP-binding component
Homo sapiens ENSG00000119688   ATP-binding cassette, sub-family D (ALD), member 4
Homo sapiens ENSG00000101986   ATP-binding cassette, sub-family D (ALD), member 1
Homo sapiens 225   ATP-binding cassette, sub-family D (ALD), member 2
Homo sapiens ENSG00000117528   ATP-binding cassette, sub-family D (ALD), member 3
Leishmania braziliensis LbrM.31.0700   hypothetical protein, conserved
Leishmania braziliensis LbrM.33.2130   ABC transporter, putative
Leishmania donovani LdBPK_331960.1   ATP-binding cassette protein subfamily D, member 3, putative
Leishmania donovani LdBPK_310560.1   ATP-binding cassette protein subfamily D, member 2, putative
Leishmania infantum LinJ.31.0560   ATP-binding cassette protein subfamily D, member 2, putative
Leishmania infantum LinJ.33.1960   ATP-binding cassette protein subfamily D, member 3, putative
Leishmania major LmjF.31.0540   ATP-binding cassette protein subfamily D, member 2, putative
Leishmania major LmjF.33.1860   ATP-binding cassette protein subfamily D, member 3, putative
Leishmania mexicana LmxM.32.1860   ABC transporter, putative
Leishmania mexicana LmxM.30.0540   ATP-binding cassette protein subfamily D, member 2, putative
Loa Loa (eye worm) LOAG_08833   hypothetical protein
Loa Loa (eye worm) LOAG_11515   ABC transporter
Mycobacterium leprae ML2084   PROBABLE DRUGS-TRANSPORT TRANSMEMBRANE ATP-BINDING PROTEIN ABC TRANSPORTER
Mus musculus ENSMUSG00000021240   ATP-binding cassette, sub-family D (ALD), member 4
Mus musculus ENSMUSG00000055782   ATP-binding cassette, sub-family D (ALD), member 2
Mus musculus ENSMUSG00000031378   ATP-binding cassette, sub-family D (ALD), member 1
Mus musculus ENSMUSG00000028127   ATP-binding cassette, sub-family D (ALD), member 3
Mycobacterium tuberculosis Rv1819c   Probable drug-transport transmembrane ATP-binding protein ABC transporter BacA
Mycobacterium ulcerans MUL_3059   drug-transport transmembrane ABC transporter ATP-binding protein
Neospora caninum NCLIV_057400   hypothetical protein
Oryza sativa 4324587   Os01g0218700
Oryza sativa 4337574   Os05g0107600
Onchocerca volvulus OVOC7390  
Saccharomyces cerevisiae YKL188C   ATP-binding cassette long-chain fatty acid transporter PXA2
Saccharomyces cerevisiae YPL147W   ATP-binding cassette long-chain fatty acid transporter PXA1
Schmidtea mediterranea mk4.007205.00  
Schmidtea mediterranea mk4.008121.02  
Schmidtea mediterranea mk4.006657.00  
Schmidtea mediterranea mk4.056024.00  
Schmidtea mediterranea mk4.023159.00  
Schmidtea mediterranea mk4.016628.00  
Schmidtea mediterranea mk4.022203.00  
Schmidtea mediterranea mk4.020858.00  
Schmidtea mediterranea mk4.009824.01  
Trypanosoma brucei gambiense Tbg972.4.4160   ABC transporter, putative,70 kDa peroxisomal membrane protein
Trypanosoma brucei gambiense Tbg972.11.3460   ABC transporter, putative,adrenoleukodystrophy protein homolog
Trypanosoma brucei Tb927.11.3130   glycosomal transporter (GAT2)
Trypanosoma brucei Tb927.4.4050   glycosomal transporter (GAT1)
Trypanosoma congolense TcIL3000_4_3540   glycosomal transporter (GAT1)
Trypanosoma congolense TcIL3000_10_13930   glycosomal transporter (GAT1)
Trypanosoma cruzi TcCLB.508927.20   glycosomal transporter (GAT1), putative
Trypanosoma cruzi TcCLB.510431.150   glycosomal transporter (GAT2), putative
Trypanosoma cruzi TcCLB.509237.30   glycosomal transporter (GAT1), putative
Toxoplasma gondii TGME49_314330   ABC transporter, ATP-binding domain-containing protein
Trichomonas vaginalis TVAG_470720   peroxisomal membrane protein, putative
Trichomonas vaginalis TVAG_605460   peroxisomal long-chain fatty acid import protein, putative

Essentiality

Tb927.11.3130 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.4050 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.4050 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.4050 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.4050 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.02.0630 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.0630 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.0630 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.0630 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b1496 Escherichia coli non-essential goodall
TGME49_314330 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.11.3130 (Trypanosoma brucei), glycosomal transporter (GAT2)
Title for this comment
Comment