Detailed view for Tb927.11.16910

Basic information

TDR Targets ID: 17591
Trypanosoma brucei, Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5758 | Length (AA): 912 | MW (Da): 99147 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01926   50S ribosome-binding GTPase
PF03959   Serine hydrolase (FSH1)
PF08438   GTPase of unknown function C-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 219 3cn9 (A) 16 213 23.00 0 0.98 0.393124 -0.02
4 219 1auo (A) 17 215 22.00 0 1 0.447124 -0.54
68 199 3cn9 (A) 85 193 36.00 0.00033 0.83 0.367048 -0.13
437 472 2ohf (A) 17 63 56.00 0.017 0.08 0.584304 -0.33
445 489 2qm8 (A) 56 102 29.00 0 0.06 0.204205 0.79
445 728 1jal (A) 3 257 24.00 0 1 0.348443 0.81
447 812 1wxq (A) 3 349 31.00 0.0000000003 1 0.394674 0.79
654 705 2ddr (A) 26 77 37.00 0.072 0.45 0.375261 0.91

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130225)

Species Accession Gene Product
Babesia bovis BBOV_IV001640   GTP binding protein, putative
Candida albicans CaO19.4846   similar to S. cerevisiae YGR210C
Candida albicans CaO19.12309   similar to S. cerevisiae YGR210C
Cryptosporidium hominis Chro.60204   GTP-binding protein
Cryptosporidium parvum cgd6_1670   MJ1332/Ygr210cp-like GTP binding protein; GTpase OBG family plus RNA binding domain TGS
Dictyostelium discoideum DDB_G0283773   hypothetical protein
Leishmania braziliensis LbrM.32.3400   hypothetical protein, conserved
Leishmania donovani LdBPK_323310.1   Serine hydrolase (FSH1)/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Leishmania infantum LinJ.32.3310   hypothetical protein, conserved
Leishmania major LmjF.32.3110   hypothetical protein, conserved
Leishmania mexicana LmxM.31.3110   hypothetical protein, conserved
Neospora caninum NCLIV_048120   GTP-binding protein, putative
Saccharomyces cerevisiae YGR210C   hypothetical protein
Trypanosoma brucei gambiense Tbg972.11.19070   hypothetical protein, conserved
Trypanosoma brucei Tb11.v5.0491   Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase, putative
Trypanosoma brucei Tb927.11.16910   Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Trypanosoma congolense TcIL3000_0_44730   Serine hydrolase (FSH1)/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Trypanosoma cruzi TcCLB.503749.10   Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Trypanosoma cruzi TcCLB.511021.99   Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Trypanosoma cruzi TcCLB.508709.10   Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Toxoplasma gondii TGME49_224600   GTP binding protein

Essentiality

Tb927.11.16910 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.8680 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.8680 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.8680 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.8680 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_224600 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.16910 (Trypanosoma brucei), Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
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