Detailed view for Tb927.10.9770

Basic information

TDR Targets ID: 17669
Trypanosoma brucei, gamma-tubulin complex component 2
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.6094 | Length (AA): 784 | MW (Da): 87084 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04130   Spc97 / Spc98 family

Gene Ontology

Mouse over links to read term descriptions.
GO:0043015   gamma-tubulin binding  
GO:0007020   microtubule nucleation  
GO:0005815   microtubule organizing center  
GO:0000922   spindle pole  
GO:0000226   microtubule cytoskeleton organization and biogenesis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128455)

Species Accession Gene Product
Arabidopsis thaliana AT5G17410   Spc97 / Spc98 family of spindle pole body (SBP) component
Babesia bovis BBOV_II001290   spc97 / spc98 family protein
Brugia malayi Bm1_24520   Spc97 / Spc98 family protein
Candida albicans CaO19.708   similar to S. cerevisiae SPC97 (YHR172W) spindle pole body component
Candida albicans CaO19.8327   similar to S. cerevisiae SPC97 (YHR172W) spindle pole body component
Cryptosporidium hominis Chro.70344   PV1H14095_P-related
Cryptosporidium parvum cgd7_3060   gamma-tubulin complex associated protein
Dictyostelium discoideum DDB_G0285849   spindle pole body component 97
Drosophila melanogaster Dmel_CG3917   Gamma-tubulin ring protein 84
Echinococcus granulosus EgrG_000621500   gamma tubulin complex component 2
Entamoeba histolytica EHI_043950   hypothetical protein
Echinococcus multilocularis EmuJ_000621500   gamma tubulin complex component 2
Giardia lamblia GL50803_17429   Tubulin, small gamma tubulin complex gcp2
Homo sapiens ENSG00000130640   tubulin, gamma complex associated protein 2
Leishmania braziliensis LbrM.35.4630   gamma-tubulin complex subunit, putative
Leishmania donovani LdBPK_364610.1   gamma-tubulin complex component 2
Leishmania infantum LinJ.36.4610   gamma-tubulin complex subunit, putative
Leishmania major LmjF.36.4390   gamma-tubulin complex subunit, putative
Leishmania mexicana LmxM.36.4390   gamma-tubulin complex subunit, putative
Loa Loa (eye worm) LOAG_06718   hypothetical protein
Mus musculus ENSMUSG00000025474   tubulin, gamma complex associated protein 2
Neospora caninum NCLIV_024430   gamma-tubulin complex component 2, putative
Oryza sativa 4336312   Os04g0501700
Onchocerca volvulus OVOC12221  
Saccharomyces cerevisiae YHR172W   Spc97p
Schistosoma japonicum Sjp_0069270   Gamma-tubulin complex component 2, putative
Schistosoma japonicum Sjp_0212770   ko:K06540 a disintegrin and metalloproteinase domain 8, putative
Schistosoma mansoni Smp_125530   gamma-tubulin complex component 2 (gcp-2)
Schmidtea mediterranea mk4.002585.02   Gamma-tubulin complex component 2
Schmidtea mediterranea mk4.002585.03   Gamma-tubulin complex component 2
Trypanosoma brucei gambiense Tbg972.10.11940   gamma-tubulin complex subunit, putative,spindle pole body protein, putative
Trypanosoma brucei Tb927.10.9770   gamma-tubulin complex component 2
Trypanosoma brucei Tb11.v5.0848   gamma-tubulin complex subunit, putative
Trypanosoma congolense TcIL3000_10_8580   gamma-tubulin complex component 2
Trypanosoma cruzi TcCLB.504147.170   gamma-tubulin complex component 2
Toxoplasma gondii TGME49_263510   Spc97 / Spc98 family protein
Trichomonas vaginalis TVAG_081520   gamma tubulin complex protein, putative
Trichomonas vaginalis TVAG_206250   gamma tubulin complex protein, putative

Essentiality

Tb927.10.9770 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.9770 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.9770 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.9770 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.9770 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
YHR172W Saccharomyces cerevisiae inviable yeastgenome
TGME49_263510 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.9770 (Trypanosoma brucei), gamma-tubulin complex component 2
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