Detailed view for Tb927.8.6810

Basic information

TDR Targets ID: 17783
Trypanosoma brucei, STE/STE11 serine/threonine-protein kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.2998 | Length (AA): 433 | MW (Da): 46851 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_141398)

Species Accession Gene Product
Leishmania braziliensis LbrM.24.1410   protein kinase, putative,NPK1-related protein kinase-like
Leishmania donovani LdBPK_241500.1   protein kinase, putative
Leishmania infantum LinJ.24.1500   protein kinase, putative,NPK1-related protein kinase-like
Leishmania major LmjF.24.1450   protein kinase, putative,NPK1-related protein kinase-like
Leishmania mexicana LmxM.24.1450   protein kinase, putative,NPK1-related protein kinase-like
Trypanosoma brucei gambiense Tbg972.8.7000   protein kinase, putative
Trypanosoma brucei Tb927.8.6810   STE/STE11 serine/threonine-protein kinase, putative
Trypanosoma congolense TcIL3000_8_6720   protein kinase, putative
Trypanosoma cruzi TcCLB.507991.20   STE/STE11 serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.506205.40   STE/STE11 serine/threonine-protein kinase, putative

Essentiality

Tb927.8.6810 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.6810 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.6810 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.6810 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.6810 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 33.0% 194 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 26.6% 293 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 26.8% 299 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 23.7% 283 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 26.1% 299 aa Compounds References
Patiria pectinifera Cdc2 300 aa 27.6% 297 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 25.6% 308 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 22.7% 384 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0012 0.5 0.5
0.0033 1 0.5
0.0016 0.5 0.5
0.0007 0.5 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0037 1 0.5
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0088 0.4477 0.5
0.0081 1 0.5
0.0056 1 0.5
0.0093 0.8828 0
0.0032 0.5 0.5
0.0063 0.7244 0.2543
0.0022 0.5 0.5
0.0059 1 1
0.0064 0.3377 0
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0011 1 0.5
0.0029 0.5 0.5
0.0092 1 0.5
0.0008 0.5 0.5
0.0062 0.6935 0
0.0059 1 1
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0027 1 0.5
0.0066 0.3101 0
0.0026 0.5 0.5
0.0039 0.5 0.5
0.0061 0.6883 0.5304
0.0036 0.5 0.5
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0069 0.3067 1
0.0059 1 1
0.0033 0.5 0.5
0.0063 1 0.5
0.0091 1 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0007 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

27 literature references were collected for this gene.

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Gene identifier Tb927.8.6810 (Trypanosoma brucei), STE/STE11 serine/threonine-protein kinase, putative
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