Detailed view for Tb927.11.2660

Basic information

TDR Targets ID: 17793
Trypanosoma brucei, Golgi reassembly stacking protein (GRASP homologue), putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.9664 | Length (AA): 499 | MW (Da): 53677 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04495   GRASP55/65 PDZ-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0003674   molecular_function  
GO:0005795   Golgi stack  
GO:0005515   protein binding  
GO:0007030   Golgi organization and biogenesis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 76 1um1 (A) 21 93 23.00 0 0.17 0.343505 -0.07
2 245 5gml (A) 0 206 33.00 0 1 0.639478 0.44
11 76 1x5r (A) 40 104 15.00 0 0.07 0.177465 0.43
11 105 4o06 (A) 124 218 21.00 0 0.54 0.404581 -0.16
16 245 4rey (A) 15 206 35.00 0 1 0.607422 0.58

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128083)

Species Accession Gene Product
Babesia bovis BBOV_IV007640   hypothetical protein
Brugia malayi Bm1_55675   GRASP55/65 family protein
Brugia malayi Bm1_54125   golgi reassembly stacking protein 2
Candida albicans CaO19.71   weak similarity to S. cerevisiae GRH1 (YDR517W) GRASP Homolog, Golgi-localized spindle checkpoint protein
Caenorhabditis elegans CELE_Y42H9AR.1   Protein Y42H9AR.1
Cryptosporidium hominis Chro.70047   Golgi reassembly stacking protein 2, possibly N-myristoylated (53.7 kD) (4I879)
Cryptosporidium parvum cgd7_340   conserved hypothetical protein
Dictyostelium discoideum DDB_G0293248   golgi reassembly stacking protein
Drosophila melanogaster Dmel_CG7809   CG7809 gene product from transcript CG7809-RA
Echinococcus granulosus EgrG_000181900   Golgi reassembly stacking protein 2
Echinococcus multilocularis EmuJ_000181900   Golgi reassembly stacking protein 2
Homo sapiens ENSG00000115806   golgi reassembly stacking protein 2, 55kDa
Homo sapiens ENSG00000114745   golgi reassembly stacking protein 1, 65kDa
Leishmania braziliensis LbrM.33.2660   Golgi reassembly stacking protein (GRASP homologue), putative
Leishmania donovani LdBPK_332510.1   Golgi reassembly stacking protein (GRASP homologue), putative
Leishmania infantum LinJ.33.2510   Golgi reassembly stacking protein (GRASP homologue), putative
Leishmania major LmjF.33.2380   Golgi reassembly stacking protein (GRASP homologue), putative
Leishmania mexicana LmxM.32.2380   Golgi reassembly stacking protein (GRASP homologue), putative
Loa Loa (eye worm) LOAG_07938   golgi reassembly stacking protein 2
Mus musculus ENSMUSG00000014959   golgi reassembly stacking protein 2
Mus musculus ENSMUSG00000032513   golgi reassembly stacking protein 1
Neospora caninum NCLIV_025630   gorasp2-prov protein, putative
Onchocerca volvulus OVOC160   Putative golgi reassembly stacking protein
Plasmodium berghei PBANKA_0501600   golgi re-assembly stacking protein, putative
Plasmodium falciparum PF3D7_1017300   golgi re-assembly stacking protein 1, golgi re-assembly stacking protein 2
Plasmodium knowlesi PKNH_0601500   golgi re-assembly stacking protein, putative
Plasmodium vivax PVX_001735   golgi re-assembly stacking protein, putative
Plasmodium yoelii PY00200   similar to golgi perepheral membrane protein p65, putative
Saccharomyces cerevisiae YDR517W   Grh1p
Schistosoma japonicum Sjp_0035410   Golgi reassembly-stacking protein 2, putative
Schistosoma mansoni Smp_169990.1   golgi reassembly stacking protein 2 (grasp2)
Schistosoma mansoni Smp_169990.2   golgi reassembly stacking protein 2 (grasp2)
Schmidtea mediterranea mk4.007473.01  
Schmidtea mediterranea mk4.006667.01  
Trypanosoma brucei gambiense Tbg972.11.2920   Golgi reassembly stacking protein (GRASP homologue), putative
Trypanosoma brucei Tb927.11.2660   Golgi reassembly stacking protein (GRASP homologue), putative
Trypanosoma congolense TcIL3000_0_24440   Golgi reassembly stacking protein (GRASP homologue), putative
Trypanosoma congolense TcIL3000.11.2410   Golgi reassembly stacking protein (GRASP homologue), putative
Trypanosoma cruzi TcCLB.506301.20   Golgi reassembly stacking protein (GRASP homologue), putative
Trypanosoma cruzi TcCLB.504153.320   Golgi reassembly stacking protein (GRASP homologue), putative
Toxoplasma gondii TGME49_261980   gorasp2-prov protein
Theileria parva TP03_0249   hypothetical protein

Essentiality

Tb927.11.2660 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.0260 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.0260 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.0260 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.0260 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_261980 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell morphogenesis (GO:0000902) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
regulation of cell motility (GO:0051270) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell cycle (GO:0007049) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
mitochondrion organization and biogenesis (GO:0007005) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
regulation of cell motility (GO:0051270) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
endocytosis (GO:0006897) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.2660 (Trypanosoma brucei), Golgi reassembly stacking protein (GRASP homologue), putative
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