Detailed view for Tb927.9.9100

Basic information

TDR Targets ID: 18037
Trypanosoma brucei, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.7254 | Length (AA): 84 | MW (Da): 9589 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0006355   regulation of transcription, DNA-dependent  
GO:0005634   nucleus  
GO:0003713   transcription coactivator activity  
GO:0003677   DNA binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 84 2fxo (A) 847 930 25.00 0.93 0.06 1.11649 0.23
19 78 5cws (C) 589 649 33.00 0.52 0.11 0.964386 0.34

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129799)

Species Accession Gene Product
Arabidopsis thaliana AT2G14045   hypothetical protein
Dictyostelium discoideum DDB_G0288991   hypothetical protein
Drosophila melanogaster Dmel_CG4546   CG4546 gene product from transcript CG4546-RA
Drosophila melanogaster Dmel_CG17202   CG17202 gene product from transcript CG17202-RA
Giardia lamblia GL50803_7007   Hypothetical protein
Homo sapiens 26292   MYC binding protein
Leishmania braziliensis LbrM.34.5200   hypothetical protein, conserved
Leishmania donovani LdBPK_355230.1   hypothetical protein, conserved
Leishmania infantum LinJ.35.5230   hypothetical protein, conserved
Leishmania major LmjF.35.5260   hypothetical protein, conserved
Leishmania mexicana LmxM.34.5260   hypothetical protein, conserved
Mus musculus ENSMUSG00000028647   c-myc binding protein
Neospora caninum NCLIV_001100   hypothetical protein
Oryza sativa 4342591   Os07g0185800
Plasmodium berghei PBANKA_1421400   C-Myc-binding protein, putative
Plasmodium falciparum PF3D7_0715100   C-Myc-binding protein, putative
Plasmodium knowlesi PKNH_1424100   C-Myc-binding protein, putative
Plasmodium vivax PVX_122962   C-Myc-binding protein, putative
Plasmodium yoelii PY01299   hypothetical protein
Schistosoma japonicum Sjp_0308020   C-Myc-binding protein, putative
Schistosoma mansoni Smp_030440   hypothetical protein
Schmidtea mediterranea mk4.015008.00   C-Myc-binding protein
Trypanosoma brucei gambiense Tbg972.9.5050   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.9100   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506883.69   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510429.24   hypothetical protein, conserved
Toxoplasma gondii TGME49_305220   C-Myc-binding family protein
Trichomonas vaginalis TVAG_253810   conserved hypothetical protein
Trichomonas vaginalis TVAG_064160   C-Myc-binding protein, putative
Trichomonas vaginalis TVAG_084840   associate of C-myc, putative
Trichomonas vaginalis TVAG_471990   C-Myc-binding protein, putative
Trichomonas vaginalis TVAG_185270   conserved hypothetical protein

Essentiality

Tb927.9.9100 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.0775 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.0775 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.0775 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.0775 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_305220 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tbru018841AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru018841; Recombinant protein: full-length; Source: T brucei; hypothetical protein, conserved ;

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.9.9100 (Trypanosoma brucei), hypothetical protein, conserved
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