Detailed view for PF3D7_1134100

Basic information

TDR Targets ID: 1821
Plasmodium falciparum, protein disulfide isomerase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.2338 | Length (AA): 423 | MW (Da): 49195 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00085   Thioredoxin

Gene Ontology

Mouse over links to read term descriptions.
GO:0005788   endoplasmic reticulum lumen  
GO:0045454   cell redox homeostasis  
GO:0006467   protein thiol-disulfide exchange  

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
161 422 2b5e (A) 29 271 16.00 0.0000000000031 1 0.8 -0.6
170 277 1gh2 (A) 9 108 22.00 0.000016 1 0.72 -2.4
22 91 2dml (A) 10 79 26.00 0.00037 0.24 0.455385 -0.19
36 263 3idv (A) 68 270 28.00 0 1 0.733507 -0.11
166 264 2dml (A) 19 111 40.00 0.0000000044 1 0.756543 -1.09
36 263 3idv (A) 68 270 28.00 0 1 0.719007 0.03
162 275 3apq (A) 127 232 32.00 0.0000036 1 0.799504 -1.87
162 268 1x5d (A) 5 108 41.00 0 1 0.839955 -1.44
188 249 1v98 (A) 55 114 33.00 0.0047 0.94 0.587572 -0.54

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, early schizont, Oocyst. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, early trophozoite, late schizont, late trophozoite, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 8 hs, gametocyte, sporozoite, early ring, late ring. Otto TD PlasmoDB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Female Gametocyte, Male Gametocyte. Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Sporozoite. Zanghi G
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.

Orthologs

Ortholog group members (OG5_127741)

Species Accession Gene Product
Arabidopsis thaliana AT2G47470   protein disulfide-isomerase like 2-1
Arabidopsis thaliana AT2G32920   protein disulfide-isomerase like 2-3
Arabidopsis thaliana AT1G04980   protein disulfide-isomerase like 2-2
Babesia bovis BBOV_IV009100   protein disulfide isomerase related protein
Brugia malayi Bm1_33495   Probable protein disulfide isomerase A6 precursor, putative
Candida albicans CaO19.2516   similar to N terminus of S. pombe SPAC17H9.14c putative protein disulfide isomerase
Candida albicans CaO19.10052   similar to N terminus of S. pombe SPAC17H9.14c putative protein disulfide isomerase
Caenorhabditis elegans CELE_B0403.4   Protein TAG-320
Caenorhabditis elegans CELE_Y49E10.4   Protein Y49E10.4
Cryptosporidium hominis Chro.70453   protein disulfide isomerase-related protein (provisional)
Cryptosporidium parvum cgd7_4080   protein disulfide isomerase, signal peptide, ER retention motif
Dictyostelium discoideum DDB_G0276141   protein disulfide isomerase
Drosophila melanogaster Dmel_CG5809   calcium-binding protein 1
Echinococcus granulosus EgrG_001002700   protein disulfide isomerase A6
Entamoeba histolytica EHI_071590   protein disulfide isomerase, putative
Echinococcus multilocularis EmuJ_001002700   protein disulfide isomerase A6
Homo sapiens ENSG00000143870   protein disulfide isomerase family A, member 6
Leishmania braziliensis LbrM.26.0670   protein disulfide isomerase, putative
Leishmania donovani LdBPK_260630.1   protein disulfide isomerase, putative
Leishmania infantum LinJ.26.0630   protein disulfide isomerase, putative
Leishmania major LmjF.26.0660   protein disulfide isomerase, putative
Leishmania mexicana LmxM.26.0660   protein disulfide isomerase, putative
Loa Loa (eye worm) LOAG_02996   TAG-320 protein
Mus musculus ENSMUSG00000020571   protein disulfide isomerase associated 6
Neospora caninum NCLIV_065470   hypothetical protein
Oryza sativa 4326806   Os01g0339900
Oryza sativa 4337858   Os05g0156300
Oryza sativa 4347226   Os09g0451500
Plasmodium berghei PBANKA_0914300   protein disulfide isomerase related protein, putative
Plasmodium falciparum PF3D7_1134100   protein disulfide isomerase
Plasmodium knowlesi PKNH_0932300   protein disulfide-isomerase, putative
Plasmodium vivax PVX_092315   protein disulfide isomerase, putative
Plasmodium yoelii PY06980   Thioredoxin, putative
Schistosoma japonicum Sjp_0207350   ko:K01829 protein disulfide-isomerase [EC5.3.4.1], putative
Schistosoma mansoni Smp_172110   shc transforming protein
Schmidtea mediterranea mk4.001731.00   Protein disulfide-isomerase A6
Schmidtea mediterranea mk4.003196.02  
Trypanosoma brucei gambiense Tbg972.7.1260   protein disulfide isomerase, putative
Trypanosoma brucei Tb927.7.1300   protein disulfide isomerase, putative
Trypanosoma congolense TcIL3000_7_920   protein disulfide isomerase, putative
Trypanosoma cruzi TcCLB.508209.140   protein disulfide isomerase, putative
Trypanosoma cruzi TcCLB.509505.10   protein disulfide isomerase, putative
Toxoplasma gondii TGME49_249270   protein disulfide isomerase-related protein (provisional), putative
Theileria parva TP01_0863   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_277120   protein disulfide isomerase, putative
Trichomonas vaginalis TVAG_117470   protein disulfide isomerase, putative

Essentiality

PF3D7_1134100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.1300 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.1300 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.1300 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.1300 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_B0403.4 Caenorhabditis elegans larval arrest wormbase
TGME49_249270 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens protein disulfide isomerase family A, member 6 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Protein Disulfide-Isomerase (5.3.4.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

  • Reagent:
  • Target Type Source Notes
    PF3D7_1134100 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Plasmodium falciparum ( 1 )

Bibliographic References

5 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_1134100 (Plasmodium falciparum), protein disulfide isomerase
Title for this comment
Comment