Detailed view for Tb927.10.3310

Basic information

TDR Targets ID: 18227
Trypanosoma brucei, Component of motile flagella 76b
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.5528 | Length (AA): 946 | MW (Da): 110240 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129277)

Species Accession Gene Product
Dictyostelium discoideum DDB_G0287291   actin binding protein D
Drosophila melanogaster Dmel_CG6059   CG6059 gene product from transcript CG6059-RA
Drosophila melanogaster Dmel_CG5882   CG5882 gene product from transcript CG5882-RA
Drosophila melanogaster Dmel_CG40446   Occludin-Related Y
Echinococcus granulosus EgrG_000954700   coiled coil domain containing protein 147
Echinococcus multilocularis EmuJ_000954700   coiled coil domain containing protein 147
Giardia lamblia GL50803_3409   Coiled-coil protein
Homo sapiens ENSG00000120051   cilia and flagella associated protein 58
Leishmania braziliensis LbrM.35.5270   hypothetical protein, conserved
Leishmania braziliensis LbrM.03.0310   hypothetical protein, conserved
Leishmania donovani LdBPK_030300.1   hypothetical protein, conserved
Leishmania donovani LdBPK_365250.1   hypothetical protein, conserved
Leishmania infantum LinJ.36.5250   hypothetical protein, conserved
Leishmania infantum LinJ.03.0300   hypothetical protein, conserved
Leishmania major LmjF.36.5020   hypothetical protein, conserved
Leishmania major LmjF.03.0320   hypothetical protein, conserved
Leishmania mexicana LmxM.03.0320   hypothetical protein, conserved
Mus musculus ENSMUSG00000046585   cilia and flagella associated protein 58
Neospora caninum NCLIV_023900   hypothetical protein
Plasmodium berghei PBANKA_1125300   conserved Plasmodium protein, unknown function
Plasmodium falciparum PF3D7_0626500   conserved Plasmodium protein, unknown function
Plasmodium knowlesi PKNH_1123300   conserved Plasmodium protein, unknown function
Plasmodium vivax PVX_114430   hypothetical protein, conserved
Plasmodium yoelii PY07530   hypothetical protein
Schistosoma japonicum Sjp_0111790   IPR010978,tRNA-binding arm,domain-containing
Schistosoma japonicum Sjp_0045650   Conserved hypothetical protein
Schistosoma mansoni Smp_156370   hypothetical protein
Schmidtea mediterranea mk4.002732.02  
Trypanosoma brucei gambiense Tbg972.10.4180   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.11.12210   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.3310   Component of motile flagella 76b
Trypanosoma brucei Tb927.11.10900   Component of motile flagella 9
Trypanosoma congolense TcIL3000.11.11580   MENG
Trypanosoma congolense TcIL3000_10_2750   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510941.20   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508179.30   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510423.70   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.510425.10   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.509825.20   hypothetical protein, conserved
Trichomonas vaginalis TVAG_470210   RAB6-interacting protein, putative

Essentiality

Tb927.10.3310 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.2670 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.2670 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.2670 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.2670 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.3310 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.3310 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.3310 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.3310 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1125300 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier Tb927.10.3310 (Trypanosoma brucei), Component of motile flagella 76b
Title for this comment
Comment