Detailed view for Tb927.9.14330

Basic information

TDR Targets ID: 18237
Trypanosoma brucei, mismatch repair protein PMS1, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.3983 | Length (AA): 788 | MW (Da): 86253 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01119   DNA mismatch repair protein, C-terminal domain
PF08676   MutL C terminal dimerisation domain
PF13589   Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

Gene Ontology

Mouse over links to read term descriptions.
GO:0032300   mismatch repair complex  
GO:0030983   mismatched DNA binding  
GO:0016887   ATPase activity  
GO:0005524   ATP binding  
GO:0006298   mismatch repair  

Metabolic Pathways

Mismatch repair (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128001)

Species Accession Gene Product
Arabidopsis thaliana AT4G02460   DNA mismatch repair protein PMS1
Babesia bovis BBOV_IV001710   DNA mismatch repair protein, putative
Brugia malayi Bm1_06100   DNA mismatch repair protein MutL containing protein
Candida albicans CaO19.9173   mismatch repair
Candida albicans CaO19.1605   mismatch repair
Caenorhabditis elegans CELE_H12C20.2   Protein PMS-2, isoform A
Cryptosporidium hominis Chro.20080   DNA mismatch repair protein, C-terminal domain
Cryptosporidium parvum cgd2_700   PMS1MutL family ATpase
Dictyostelium discoideum DDB_G0283981   MutL DNA mismatch repair protein
Drosophila melanogaster Dmel_CG8169   CG8169 gene product from transcript CG8169-RA
Echinococcus granulosus EgrG_000887200   mismatch repair endonuclease PMS2
Entamoeba histolytica EHI_155170   DNA mismatch repair protein PMS1, putative
Echinococcus multilocularis EmuJ_000887200   mismatch repair endonuclease PMS2
Giardia lamblia GL50803_14195   Pms1-like protein
Homo sapiens ENSG00000122512   PMS2 postmeiotic segregation increased 2 (S. cerevisiae)
Leishmania braziliensis LbrM.34.1570   mismatch repair protein PMS1, putative,mismatch repair protein
Leishmania donovani LdBPK_351660.1   mismatch repair protein PMS1, putative
Leishmania infantum LinJ.35.1660   mismatch repair protein PMS1, putative,mismatch repair protein
Leishmania major LmjF.35.1660   mismatch repair protein PMS1, putative,mismatch repair protein
Leishmania mexicana LmxM.34.1660   mismatch repair protein PMS1, putative,mismatch repair protein
Loa Loa (eye worm) LOAG_09882   hypothetical protein
Loa Loa (eye worm) LOAG_10077   hypothetical protein
Mus musculus 18861   postmeiotic segregation increased 2 (S. cerevisiae)
Neospora caninum NCLIV_050460   hypothetical protein
Oryza sativa 4329835   Os02g0592300
Plasmodium berghei PBANKA_0210400   DNA mismatch repair protein PMS1, putative
Plasmodium falciparum PF3D7_0726300   DNA mismatch repair protein PMS1, putative
Plasmodium knowlesi PKNH_0210900   DNA mismatch repair protein PMS1, putative
Plasmodium vivax PVX_081620   DNA mismatch repair protein PMS1, putative
Plasmodium yoelii PY01438   DNA mismatch repair protein, C-terminal domain, putative
Saccharomyces cerevisiae YNL082W   Pms1p
Schistosoma japonicum Sjp_0049620   Mismatch repair endonuclease PMS2, putative
Schistosoma japonicum Sjp_0104610   Mismatch repair endonuclease PMS2, putative
Schistosoma japonicum Sjp_0303870   Mismatch repair endonuclease PMS2, putative
Schistosoma mansoni Smp_143380   DNA mismatch repair protein PMS2
Schmidtea mediterranea mk4.014210.01   Mismatch repair endonuclease PMS2
Schmidtea mediterranea mk4.014210.00   Mismatch repair endonuclease PMS2
Schmidtea mediterranea mk4.005186.08  
Schmidtea mediterranea mk4.009313.02   Mismatch repair endonuclease PMS2
Schmidtea mediterranea mk4.009313.00  
Schmidtea mediterranea mk4.009313.01   Mismatch repair endonuclease PMS2
Trypanosoma brucei gambiense Tbg972.9.9020   mismatch repair protein PMS1, putative
Trypanosoma brucei Tb927.9.14330   mismatch repair protein PMS1, putative
Trypanosoma congolense TcIL3000_9_6160   mismatch repair protein PMS1, putative
Trypanosoma cruzi TcCLB.510761.10   mismatch repair protein PMS1, putative
Toxoplasma gondii TGME49_236200   DNA mismatch repair protein, C-terminal domain-containing protein
Theileria parva TP03_0491   DNA mismatch repair protein PMS1, putative
Trichomonas vaginalis TVAG_005070   mismatch repair protein Pms1 (mutL homolog)

Essentiality

Tb927.9.14330 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.4840 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.4840 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.4840 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.4840 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_H12C20.2 Caenorhabditis elegans embryonic lethal wormbase
PBANKA_0210400 Plasmodium berghei Essential plasmo
TGME49_236200 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.9.14330 (Trypanosoma brucei), mismatch repair protein PMS1, putative
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