Detailed view for Tb927.9.11490

Basic information

TDR Targets ID: 18291
Trypanosoma brucei, 60S ribosomal protein L29
Source Database / ID:  TriTrypDB  GeneDB

pI: 11.1895 | Length (AA): 145 | MW (Da): 16266 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00828   Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

Gene Ontology

Mouse over links to read term descriptions.
GO:0022625   cytosolic large ribosomal subunit  
GO:0015934   large ribosomal subunit  
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0003723   RNA binding  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

PDB tag
  • 3ZF7:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127016)

Species Accession Gene Product
Arabidopsis thaliana AT1G70600   60S ribosomal protein L27a-3
Arabidopsis thaliana AT1G23290   60S ribosomal protein L27a-2
Babesia bovis BBOV_III006560   ribosomal protein L15, putative
Brugia malayi Bm1_04910   60S ribosomal protein L27a
Caenorhabditis elegans CELE_Y37E3.8   Protein Y37E3.8, isoform A
Cryptosporidium hominis Chro.30443   ribosomal protein L22
Cryptosporidium parvum cgd3_3930   60S ribosomal protein L27A or L27a
Dictyostelium discoideum DDB_G0292388   S60 ribosomal protein L27a
Drosophila melanogaster Dmel_CG15442   Ribosomal protein L27A
Entamoeba histolytica EHI_169100   ribosomal protein L27a, putative
Entamoeba histolytica EHI_069490   ribosomal protein L27a, putative
Entamoeba histolytica EHI_192090   ribosomal protein L27a, putative
Giardia lamblia GL50803_16310   Ribosomal protein L27a
Homo sapiens ENSG00000166441   ribosomal protein L27a
Leishmania braziliensis LbrM.34.3740   60S ribosomal protein L27A/L29, putative
Leishmania braziliensis LbrM.34.3760   60S ribosomal protein L27A/L29, putative
Leishmania donovani LdBPK_353810.1   60S ribosomal protein L27A/L29, putative
Leishmania infantum LinJ.35.3830   60S ribosomal protein L27A/L29, putative
Leishmania infantum LinJ.35.3810   60S ribosomal protein L27A/L29, putative
Leishmania major LmjF.35.3760   60S ribosomal protein L27A/L29, putative
Leishmania major LmjF.35.3780   60S ribosomal protein L27A/L29, putative
Leishmania mexicana LmxM.34.3760   60S ribosomal protein L27A/L29, putative
Leishmania mexicana LmxM.34.3780   60S ribosomal protein L27A/L29, putative
Loa Loa (eye worm) LOAG_09561   60S ribosomal protein L27a
Mus musculus 546052   predicted gene 5908
Mus musculus ENSMUSG00000046364   ribosomal protein L27A
Neospora caninum NCLIV_054570   60S ribosomal protein Ll28B 27a, related
Oryza sativa 4328472   Os02g0175600
Oryza sativa 4333083   Os03g0408300
Oryza sativa 4343884   Os07g0613200
Onchocerca volvulus OVOC1439   60S ribosomal protein L27a homolog
Plasmodium berghei PBANKA_1117500   ribosomal protein L27a, putative
Plasmodium falciparum PF3D7_0618300   60S ribosomal protein L27a, putative
Plasmodium knowlesi PKNH_1132100   60S ribosomal protein L27a, putative
Plasmodium vivax PVX_114040   60S ribosomal protein L27a, putative
Plasmodium yoelii PY03375   ribosomal protein L27a
Saccharomyces cerevisiae YGL103W   ribosomal 60S subunit protein L28
Schmidtea mediterranea mk4.037030.00   60S ribosomal protein L27a
Trypanosoma brucei gambiense Tbg972.9.6830   60S ribosomal protein L27a,60S ribosomal protein L28,60S ribosomal protein L29, putative
Trypanosoma brucei gambiense Tbg972.9.6810   60S ribosomal protein L27a,60S ribosomal protein L28,60S ribosomal protein L29
Trypanosoma brucei Tb927.9.11490   60S ribosomal protein L29
Trypanosoma brucei Tb427tmp.211.2650   60S ribosomal protein L27a
Trypanosoma congolense TcIL3000_0_56640   60S ribosomal protein L27a
Trypanosoma cruzi TcCLB.508461.510   60S ribosomal protein L27A/L29, putative
Trypanosoma cruzi TcCLB.508461.500   60S ribosomal protein L27A/L29, putative
Toxoplasma gondii TGME49_310490   ribosomal protein RPL27A
Theileria parva TP02_0317   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_143790   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_121550   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_401890   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_346410   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_199230   60S ribosomal protein L27a, putative
Trichomonas vaginalis TVAG_300490   60S ribosomal protein L27a, putative

Essentiality

Tb927.9.11490 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.2650 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.2650 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.2650 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.2650 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y37E3.8 Caenorhabditis elegans embryonic lethal wormbase
YGL103W Saccharomyces cerevisiae inviable yeastgenome
TGME49_310490 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.9.11490 (Trypanosoma brucei), 60S ribosomal protein L29
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