Detailed view for Tb927.11.14070

Basic information

TDR Targets ID: 18336
Trypanosoma brucei, Repressor of differentiation kinase 1
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.9691 | Length (AA): 1195 | MW (Da): 131034 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 3

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0016021   integral to membrane  
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_140526)

Species Accession Gene Product
Leishmania braziliensis LbrM.32.0900   serine/threonine protein kinase, putative
Leishmania braziliensis LbrM.33.2580   protein kinase, putative
Leishmania donovani LdBPK_332420.1   protein kinase, putative
Leishmania donovani LdBPK_320860.1   Repressor of differentiation kinase 1, putative
Leishmania infantum LinJ.32.0860   serine/threonine protein kinase, putative
Leishmania major LmjF.32.0810   serine/threonine protein kinase, putative
Leishmania major LmjF.33.2290   protein kinase, putative
Leishmania mexicana LmxM.31.0810   serine/threonine protein kinase, putative
Leishmania mexicana LmxM.32.2290   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.11.15700   protein kinase, putative
Trypanosoma brucei Tb927.11.14070   Repressor of differentiation kinase 1
Trypanosoma cruzi TcCLB.506811.180   STE/STE11 serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.506303.60   STE/STE11 serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.511727.210   Repressor of differentiation kinase 1, putative
Trypanosoma cruzi TcCLB.511727.230   Repressor of differentiation kinase 1, putative

Essentiality

Tb927.11.14070 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.5650 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.5650 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.5650 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.5650 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 24.3% 280 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 29.4% 163 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0064 0.3377 0
0.0063 0.7244 0.2543
0.0059 1 1
0.0022 0.5 0.5
0.0093 0.8828 0
0.0032 0.5 0.5
0.0088 0.4477 0.5
0.0081 1 0.5
0.0056 1 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0032 0.5 0.5
0.0039 0.5 0.5
0.0037 1 0.5
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0033 1 0.5
0.0007 0.5 0.5
0.0033 0.5 0.5
0.0063 1 0.5
0.0004 0.5 0.5
0.0081 0.5 0.5
0.0091 1 0.5
0.0069 0.3067 1
0.0059 1 1
0.0042 0.5 0.5
0.0018 0.5 0.5
0.0026 0.5 0.5
0.0039 0.5 0.5
0.0036 0.5 0.5
0.0061 0.6883 0.5304
0.0027 1 0.5
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0066 0.3101 0
0.0062 0.6935 0
0.0059 1 1
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0008 0.5 0.5
0.0029 0.5 0.5
0.0011 1 0.5
0.0092 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

27 literature references were collected for this gene.

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Gene identifier Tb927.11.14070 (Trypanosoma brucei), Repressor of differentiation kinase 1
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