Detailed view for Tb927.9.10830

Basic information

TDR Targets ID: 18358
Trypanosoma brucei, metal dependent 5???-nucleotidase
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.1656 | Length (AA): 182 | MW (Da): 20983 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13023   HD domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0003674   molecular_function  
GO:0003824   catalytic activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 180 4l1j (A) 16 194 46.00 0 1 1.67152 -1.56

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127807)

Species Accession Gene Product
Arabidopsis thaliana AT2G23820   Metal-dependent phosphohydrolase
Arabidopsis thaliana AT1G26160   Metal-dependent phosphohydrolase
Brugia malayi Bm1_27985   HD domain containing protein
Candida albicans CaO19.10382   similar to S. cerevisiae YGL101W
Candida albicans CaO19.2426   similar to S. cerevisiae YGL101W
Candida albicans CaO19.9964   similar to S. cerevisiae YGL101W
Candida albicans CaO19.2864   similar to S. cerevisiae YGL101W
Caenorhabditis elegans CELE_F45F2.9   Protein F45F2.9, isoform A
Dictyostelium discoideum DDB_G0290795   HD domain-containing protein 2
Drosophila melanogaster Dmel_CG11050   CG11050 gene product from transcript CG11050-RA
Echinococcus granulosus EgrG_000690400   Metal dependent phosphohydrolase HD region
Echinococcus granulosus EgrG_000690600   Metal dependent phosphohydrolase HD region
Entamoeba histolytica EHI_175070   metal dependent phosphohydrolase, putative
Echinococcus multilocularis EmuJ_000690600   Metal dependent phosphohydrolase, HD region
Echinococcus multilocularis EmuJ_000690400   Metal dependent phosphohydrolase, HD region
Giardia lamblia GL50803_21592   hypothetical protein
Homo sapiens 51020   HD domain containing 2
Leishmania braziliensis LbrM.34.4100   hypothetical protein, conserved
Leishmania donovani LdBPK_354170.1   HD domain containing protein, putative
Leishmania infantum LinJ.35.4170   hypothetical protein, conserved
Leishmania major LmjF.35.4110   hypothetical protein, conserved
Leishmania mexicana LmxM.34.4110   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_10607   HD domain-containing protein
Mus musculus ENSMUSG00000000295   HD domain containing 2
Neospora caninum NCLIV_053700   HD domain-containing protein, related
Oryza sativa 4326555   Os01g0641100
Oryza sativa 4328498   Os02g0179100
Onchocerca volvulus OVOC3318  
Saccharomyces cerevisiae YBR242W   hypothetical protein
Saccharomyces cerevisiae YGL101W   hypothetical protein
Schistosoma japonicum Sjp_0014520   ko:K07023 putative hydrolases of HD superfamily, putative
Schmidtea mediterranea mk4.003763.02  
Schmidtea mediterranea mk4.003763.03  
Trypanosoma brucei gambiense Tbg972.9.6360   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.10830   metal dependent 5???-nucleotidase
Trypanosoma congolense TcIL3000_9_4390   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.508461.150   metal dependent 5???-nucleotidase
Toxoplasma gondii TGME49_309290   hypothetical protein
Trichomonas vaginalis TVAG_171610   conserved hypothetical protein

Essentiality

Tb927.9.10830 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.2190 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.2190 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.2190 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb09.211.2190 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F45F2.9 Caenorhabditis elegans sterile wormbase
TGME49_309290 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Tbru018982AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Tbru018982; Recombinant protein: full-length; Source: T brucei; hypothetical protein, conserved ;

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.9.10830 (Trypanosoma brucei), metal dependent 5???-nucleotidase
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