Detailed view for PF3D7_1136800

Basic information

TDR Targets ID: 1848
Plasmodium falciparum, DnaJ protein, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 9.416 | Length (AA): 369 | MW (Da): 44528 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00226   DnaJ domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0051082   unfolded protein binding  
GO:0031072   heat shock protein binding  
GO:0006457   protein folding  

Structural information

Modbase 3D models:

There are 13 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
90 189 2ctw (A) 3 109 28.00 0 1 0.66 -0.41
98 201 1gh6 (A) 7 113 20.00 0 0.68 0.54 -0.17
101 164 2ctp (A) 6 69 48.00 0.0000000000047 1 1.02 -2.07
4 41 4gco (A) 139 176 26.00 0.48 0.93 0.529681 -0.87
6 76 4gcn (A) 3 73 17.00 0.97 0.74 0.531112 -1.08
9 158 2y4t (A) 269 453 22.00 0.011 1 0.483604 0.01
102 171 3ucs (C) 4 73 36.00 0.034 1 0.869802 -2.32
102 201 4j80 (A) 5 104 35.00 0.000031 1 0.845103 -1.36
103 198 4j7z (A) 6 97 47.00 0.00000000012 1 0.736163 -1.24
4 41 4gco (A) 139 176 26.00 0.14 0.92 0.522081 -0.82
8 68 3lca (A) 127 187 16.00 0.7 0.27 0.488412 -1.51
101 165 2ctq (A) 19 84 40.00 0.00058 1 0.856752 -1.99
102 233 3apq (A) 34 162 26.00 0.095 1 0.673024 -0.44

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Sporozoite. Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, sporozoite, early ring, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile early schizont, early trophozoite, late trophozoite, Oocyst, Male Gametocyte. PlasmoDB Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Female Gametocyte. Lasonder E
Show/Hide expression data references
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127638)

Species Accession Gene Product
Arabidopsis thaliana AT5G49060   chaperone protein dnaJ 49
Arabidopsis thaliana AT5G05750   DNAJ heat shock N-terminal domain-containing protein
Arabidopsis thaliana AT3G57340   DnaJ/Hsp40 domain-containing protein
Babesia bovis BBOV_IV011520   DnaJ domain containing protein
Brugia malayi Bm1_26280   DnaJ domain containing protein
Candida albicans CaO19.7175   DnaJ-like protein similar to S. cerevisiae HLJ1 (YMR161W) tail-anchored ER membrane protein
Candida albicans CaO19_7175   hypothetical protein
Caenorhabditis elegans CELE_B0035.14   Protein DNJ-1, isoform B
Cryptosporidium hominis Chro.50028   CG3061-PA
Cryptosporidium parvum cgd5_3430   DNAj protein with possible transmembrane domain within C-terminal region
Dictyostelium discoideum DDB_G0281775   hypothetical protein
Drosophila melanogaster Dmel_CG3061   CG3061 gene product from transcript CG3061-RA
Echinococcus granulosus EgrG_000673700   DnaJ protein subfamily B 2
Echinococcus multilocularis EmuJ_000673700   DnaJ protein subfamily B 2
Homo sapiens ENSG00000164031   DnaJ (Hsp40) homolog, subfamily B, member 14
Homo sapiens ENSG00000148719   DnaJ (Hsp40) homolog, subfamily B, member 12
Leishmania braziliensis LbrM.35.2340   hypothetical protein, conserved
Leishmania donovani LdBPK_362230.1   chaperone protein DnaJ, putative
Leishmania infantum LinJ.36.2230   hypothetical protein, conserved
Leishmania major LmjF.36.2110   hypothetical protein, conserved
Leishmania mexicana LmxM.36.2110   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_13030   DnaJ domain-containing protein
Loa Loa (eye worm) LOAG_14276   DnaJ domain-containing protein
Mus musculus ENSMUSG00000020109   DnaJ (Hsp40) homolog, subfamily B, member 12
Mus musculus 70604   DnaJ (Hsp40) homolog, subfamily B, member 14
Neospora caninum NCLIV_026920   hypothetical protein
Oryza sativa 4324566   Os01g0556400
Oryza sativa 4338551   Os05g0364500
Onchocerca volvulus OVOC5639  
Plasmodium berghei PBANKA_0911800   DnaJ protein, putative
Plasmodium falciparum PF3D7_1136800   DnaJ protein, putative
Plasmodium knowlesi PKNH_0935000   DnaJ protein, putative
Plasmodium vivax PVX_092455   DnaJ domain containing protein
Plasmodium yoelii PY05753   DNAJ-like protein, putative
Saccharomyces cerevisiae YMR161W   Hlj1p
Schistosoma japonicum Sjp_0217370   ko:K09518 DnaJ homolog, subfamily B, member 12, putative
Schistosoma mansoni Smp_132870   DNAj homolog subfamily B member
Schmidtea mediterranea mk4.007709.01  
Schmidtea mediterranea mk4.005228.00  
Trypanosoma brucei gambiense Tbg972.10.8080   chaperone protein DNAj, putative
Trypanosoma brucei Tb927.10.6610   chaperone protein DnaJ, putative
Trypanosoma congolense TcIL3000_10_5680   chaperone protein DnaJ, putative
Trypanosoma cruzi TcCLB.510187.330   hypothetical protein, conserved
Toxoplasma gondii TGME49_259980   DnaJ domain-containing protein
Theileria parva TP01_1152   hypothetical protein

Essentiality

PF3D7_1136800 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.6610 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.6610 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.6610 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.6610 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_0911800 Plasmodium berghei Essential plasmo
TGME49_259980 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier PF3D7_1136800 (Plasmodium falciparum), DnaJ protein, putative
Title for this comment
Comment